Multiomicssubstrates of resistance to emerging pathogens? transcriptome and proteome profile of a vancomycin-resistant Enterococcus faecalis clinical strain
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/6540 |
Resumo: | Antibiotic resistance and hospital acquired infections are on the rise worldwide. Vancomycin-resistant enterococci have been reported in clinical settings in recent decades. In this multiomics study, we provide comprehensive proteomic and transcriptomic analyses of a vancomycin-resistant Enterococcus faecalis clinical isolate from a patient with a urinary tract infection. The previous genotypic profile of the strain C2620 indicated the presence of antibiotic resistance genes characteristic of the vanB cluster. To further investigate the transcriptome of this pathogenic strain, we used whole genome sequencing and RNA-sequencing to detect and quantify the genes expressed. In parallel, we used two-dimensional gel electrophoresis followed by MALDI-TOF/MS (Matrix-assisted laser desorption/ionization-Time-of-flight/Mass spectrometry) to identify the proteins in the proteome. We studied the membrane and cytoplasm subproteomes separately. From a total of 207 analysis spots, we identified 118 proteins. The protein list was compared to the results obtained from the full transcriptome assay. Several genes and proteins related to stress and cellular response were identified, as well as some linked to antibiotic and drug responses, which is consistent with the known state of multiresistance. Even though the correlation between transcriptome and proteome data is not yet fully understood, the use of multiomics approaches has proven to be increasingly relevant to achieve deeper insights into the survival ability of pathogenic bacteria found in health care facilities. |
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Multiomicssubstrates of resistance to emerging pathogens? transcriptome and proteome profile of a vancomycin-resistant Enterococcus faecalis clinical strainEnterococcusAntibiotic ResistanceBiomarkersMultiomicsProteomicsTranscriptomicsTranslational ResearchResistência aos AntimicrobianosGenómica Funcional e EstruturalAntibiotic resistance and hospital acquired infections are on the rise worldwide. Vancomycin-resistant enterococci have been reported in clinical settings in recent decades. In this multiomics study, we provide comprehensive proteomic and transcriptomic analyses of a vancomycin-resistant Enterococcus faecalis clinical isolate from a patient with a urinary tract infection. The previous genotypic profile of the strain C2620 indicated the presence of antibiotic resistance genes characteristic of the vanB cluster. To further investigate the transcriptome of this pathogenic strain, we used whole genome sequencing and RNA-sequencing to detect and quantify the genes expressed. In parallel, we used two-dimensional gel electrophoresis followed by MALDI-TOF/MS (Matrix-assisted laser desorption/ionization-Time-of-flight/Mass spectrometry) to identify the proteins in the proteome. We studied the membrane and cytoplasm subproteomes separately. From a total of 207 analysis spots, we identified 118 proteins. The protein list was compared to the results obtained from the full transcriptome assay. Several genes and proteins related to stress and cellular response were identified, as well as some linked to antibiotic and drug responses, which is consistent with the known state of multiresistance. Even though the correlation between transcriptome and proteome data is not yet fully understood, the use of multiomics approaches has proven to be increasingly relevant to achieve deeper insights into the survival ability of pathogenic bacteria found in health care facilities.Mary Ann LiebertRepositório Científico do Instituto Nacional de SaúdePinto, LuísTorres, CarmenGil, ConchaSantos, Hugo M.Capelo, José LuísBorges, VítorGomes, João PauloSilva, CatarinaVieira, LuísPoeta, PatríciaIgrejas, Gilberto2020-04-28T19:59:12Z2020-02-142020-02-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6540engOMICS. 2020 Feb 14;24(2):81-95. doi: 10.1089/omi.2019.01641536-231010.1089/omi.2019.0164info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:43Zoai:repositorio.insa.pt:10400.18/6540Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:41:34.630187Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Multiomicssubstrates of resistance to emerging pathogens? transcriptome and proteome profile of a vancomycin-resistant Enterococcus faecalis clinical strain |
title |
Multiomicssubstrates of resistance to emerging pathogens? transcriptome and proteome profile of a vancomycin-resistant Enterococcus faecalis clinical strain |
spellingShingle |
Multiomicssubstrates of resistance to emerging pathogens? transcriptome and proteome profile of a vancomycin-resistant Enterococcus faecalis clinical strain Pinto, Luís Enterococcus Antibiotic Resistance Biomarkers Multiomics Proteomics Transcriptomics Translational Research Resistência aos Antimicrobianos Genómica Funcional e Estrutural |
title_short |
Multiomicssubstrates of resistance to emerging pathogens? transcriptome and proteome profile of a vancomycin-resistant Enterococcus faecalis clinical strain |
title_full |
Multiomicssubstrates of resistance to emerging pathogens? transcriptome and proteome profile of a vancomycin-resistant Enterococcus faecalis clinical strain |
title_fullStr |
Multiomicssubstrates of resistance to emerging pathogens? transcriptome and proteome profile of a vancomycin-resistant Enterococcus faecalis clinical strain |
title_full_unstemmed |
Multiomicssubstrates of resistance to emerging pathogens? transcriptome and proteome profile of a vancomycin-resistant Enterococcus faecalis clinical strain |
title_sort |
Multiomicssubstrates of resistance to emerging pathogens? transcriptome and proteome profile of a vancomycin-resistant Enterococcus faecalis clinical strain |
author |
Pinto, Luís |
author_facet |
Pinto, Luís Torres, Carmen Gil, Concha Santos, Hugo M. Capelo, José Luís Borges, Vítor Gomes, João Paulo Silva, Catarina Vieira, Luís Poeta, Patrícia Igrejas, Gilberto |
author_role |
author |
author2 |
Torres, Carmen Gil, Concha Santos, Hugo M. Capelo, José Luís Borges, Vítor Gomes, João Paulo Silva, Catarina Vieira, Luís Poeta, Patrícia Igrejas, Gilberto |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Pinto, Luís Torres, Carmen Gil, Concha Santos, Hugo M. Capelo, José Luís Borges, Vítor Gomes, João Paulo Silva, Catarina Vieira, Luís Poeta, Patrícia Igrejas, Gilberto |
dc.subject.por.fl_str_mv |
Enterococcus Antibiotic Resistance Biomarkers Multiomics Proteomics Transcriptomics Translational Research Resistência aos Antimicrobianos Genómica Funcional e Estrutural |
topic |
Enterococcus Antibiotic Resistance Biomarkers Multiomics Proteomics Transcriptomics Translational Research Resistência aos Antimicrobianos Genómica Funcional e Estrutural |
description |
Antibiotic resistance and hospital acquired infections are on the rise worldwide. Vancomycin-resistant enterococci have been reported in clinical settings in recent decades. In this multiomics study, we provide comprehensive proteomic and transcriptomic analyses of a vancomycin-resistant Enterococcus faecalis clinical isolate from a patient with a urinary tract infection. The previous genotypic profile of the strain C2620 indicated the presence of antibiotic resistance genes characteristic of the vanB cluster. To further investigate the transcriptome of this pathogenic strain, we used whole genome sequencing and RNA-sequencing to detect and quantify the genes expressed. In parallel, we used two-dimensional gel electrophoresis followed by MALDI-TOF/MS (Matrix-assisted laser desorption/ionization-Time-of-flight/Mass spectrometry) to identify the proteins in the proteome. We studied the membrane and cytoplasm subproteomes separately. From a total of 207 analysis spots, we identified 118 proteins. The protein list was compared to the results obtained from the full transcriptome assay. Several genes and proteins related to stress and cellular response were identified, as well as some linked to antibiotic and drug responses, which is consistent with the known state of multiresistance. Even though the correlation between transcriptome and proteome data is not yet fully understood, the use of multiomics approaches has proven to be increasingly relevant to achieve deeper insights into the survival ability of pathogenic bacteria found in health care facilities. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-28T19:59:12Z 2020-02-14 2020-02-14T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/6540 |
url |
http://hdl.handle.net/10400.18/6540 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
OMICS. 2020 Feb 14;24(2):81-95. doi: 10.1089/omi.2019.0164 1536-2310 10.1089/omi.2019.0164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert |
publisher.none.fl_str_mv |
Mary Ann Liebert |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132160749207552 |