mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence potential

Detalhes bibliográficos
Autor(a) principal: Gaio, Vânia Silva
Data de Publicação: 2022
Outros Autores: Lima, Tânia, Vilanova, Manuel, Cerca, Nuno, França, Ângela Maria Oliveira Sousa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/75652
Resumo: The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021.803134/ full#supplementary-material
id RCAP_bd884a9f90f036b36488fc49517f364a
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/75652
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence potentialSERP1681SERP1682antimicrobial tolerancebiofilmshuman bloodhuman plasmamacrophagesdendritic cellsScience & TechnologyThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021.803134/ full#supplementary-materialStaphylococcus epidermidis biofilm cells are characterized by increased antimicrobial tolerance and improved ability to evade host immune system defenses. These features are, in part, due to the presence of viable but non-culturable (VBNC) cells. A previous study identified genes potentially involved in VBNC cells formation in S. epidermidis biofilms, among which SERP1682/1681 raised special interest due to their putative role as a toxinantitoxin system of the mazEF family. Herein, we constructed an S. epidermidis mutant lacking the mazEF genes homologues and determined their role in (i) VBNC state induction during biofilm formation, (ii) antimicrobial susceptibility, (iii) survival in human blood and plasma, and (iv) activation of immune cells. Our results revealed that mazEF homologue did not affect the proportion of VBNC cells in S. epidermidis 1457, refuting the previous hypothesis that mazEF homologue could be linked with the emergence of VBNC cells in S. epidermidis biofilms. Additionally, mazEF homologue did not seem to influence key virulence factors on this strain, since its deletion did not significantly affect the mutant biofilm formation capacity, antimicrobial tolerance or the response by immune cells. Surprisingly, our data suggest that mazEF does not behave as a toxinantitoxin system in S. epidermidis strain 1457, since no decrease in the viability and culturability of bacteria was found when only the mazF toxin homologue was being expressed.This work was supported by the Portuguese Foundation for Science and Technology (FCT) by the funder project PTDC/BIA-MOL/29553/2017, under the scope of COMPETE2020 (POCI-01-0145-FEDER-029553) and by the strategic funding unit UIDB/04469/2020. VG acknowledges the support of FCT individual fellowship [SFRH/BD/131452/2017].info:eu-repo/semantics/publishedVersionFrontiers Media S. A.Universidade do MinhoGaio, Vânia SilvaLima, TâniaVilanova, ManuelCerca, NunoFrança, Ângela Maria Oliveira Sousa2022-01-132022-01-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/75652engGaio, Vania; Lima, Tânia; Vilanova, Manuel; Cerca, Nuno; França, Angela, mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence potential. Frontiers in Cellular and Infection Microbiology, 11(803134), 20222235-298810.3389/fcimb.2021.80313435096651803134https://www.frontiersin.org/journals/cellular-and-infection-microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:55Zoai:repositorium.sdum.uminho.pt:1822/75652Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:53.444894Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence potential
title mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence potential
spellingShingle mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence potential
Gaio, Vânia Silva
SERP1681
SERP1682
antimicrobial tolerance
biofilms
human blood
human plasma
macrophages
dendritic cells
Science & Technology
title_short mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence potential
title_full mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence potential
title_fullStr mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence potential
title_full_unstemmed mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence potential
title_sort mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence potential
author Gaio, Vânia Silva
author_facet Gaio, Vânia Silva
Lima, Tânia
Vilanova, Manuel
Cerca, Nuno
França, Ângela Maria Oliveira Sousa
author_role author
author2 Lima, Tânia
Vilanova, Manuel
Cerca, Nuno
França, Ângela Maria Oliveira Sousa
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gaio, Vânia Silva
Lima, Tânia
Vilanova, Manuel
Cerca, Nuno
França, Ângela Maria Oliveira Sousa
dc.subject.por.fl_str_mv SERP1681
SERP1682
antimicrobial tolerance
biofilms
human blood
human plasma
macrophages
dendritic cells
Science & Technology
topic SERP1681
SERP1682
antimicrobial tolerance
biofilms
human blood
human plasma
macrophages
dendritic cells
Science & Technology
description The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021.803134/ full#supplementary-material
publishDate 2022
dc.date.none.fl_str_mv 2022-01-13
2022-01-13T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/75652
url http://hdl.handle.net/1822/75652
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gaio, Vania; Lima, Tânia; Vilanova, Manuel; Cerca, Nuno; França, Angela, mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence potential. Frontiers in Cellular and Infection Microbiology, 11(803134), 2022
2235-2988
10.3389/fcimb.2021.803134
35096651
803134
https://www.frontiersin.org/journals/cellular-and-infection-microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S. A.
publisher.none.fl_str_mv Frontiers Media S. A.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132292731371520

Registros relacionados