Evaluation of bioactive potential of a secondary metabolite produced by Penicillium nordicum

Detalhes bibliográficos
Autor(a) principal: Wierzbínska, Agata Karolina
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/14396
Resumo: Fungi of the genus Penicillium are promising sources of bioactive substances. Fungal strains isolated from poorly characterized habitats are currently under active investigation as potential producers of biologically active compounds. Penicillium nordicum is mostly known by its ability to produce the mycotoxin ochratoxin A in several protein- and salt-rich food matrices like processed meat and cheese, but it can also produce an array of other secondary metabolites. In a previous study, strains of this species were found to produce, under specific growth conditions, one unidentified compound in apparently big amounts, without producing ochratoxin A. The aim of the present study was to evaluate the bioactive properties of this compound in terms of antioxidant, antimicrobial, cytotoxic and phytotoxic properties. For that purpose, the fungus was grown on chorizo-based medium and the aqueous extract of the compound was used for further tests. Pestalotin was the predominant compound in the fungal extract, with questiomycin and 7-hydroxypestalotin also being found at lower concentrations, as detected by Liquid Chromatography-Mass Spectrometry (HPLC-MS/MS) based multimetabolite method. The extract did not show any cytotoxic activity in non-tumor porcine liver primary culture cells and human tumor cell lines. The antibacterial effect on human clinical isolates and antifungal effect on foodborne and environme ntal isolates was also not significant. However, it showed significant effects on seed germination as well as on root and shoot growth of tomato, lettuce, wheat and oat. The root growth inhibition ranged from 65.9% to 100%, and the shoot growth was reduced in 22.7% to 92%, being tomato the most sensitive plant. The extract can be considered as a potential bioherbicide by its phytotoxic effect, without noticeable negative effects on human and animal cells and also on the environment. Further tests on herbicidal potential are now under development.
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spelling Evaluation of bioactive potential of a secondary metabolite produced by Penicillium nordicumPenicillium nordicumCytotoxicityAntioxidantAntimicrobialBioherbicideDomínio/Área Científica::Ciências Agrárias::Biotecnologia Agrária e AlimentarFungi of the genus Penicillium are promising sources of bioactive substances. Fungal strains isolated from poorly characterized habitats are currently under active investigation as potential producers of biologically active compounds. Penicillium nordicum is mostly known by its ability to produce the mycotoxin ochratoxin A in several protein- and salt-rich food matrices like processed meat and cheese, but it can also produce an array of other secondary metabolites. In a previous study, strains of this species were found to produce, under specific growth conditions, one unidentified compound in apparently big amounts, without producing ochratoxin A. The aim of the present study was to evaluate the bioactive properties of this compound in terms of antioxidant, antimicrobial, cytotoxic and phytotoxic properties. For that purpose, the fungus was grown on chorizo-based medium and the aqueous extract of the compound was used for further tests. Pestalotin was the predominant compound in the fungal extract, with questiomycin and 7-hydroxypestalotin also being found at lower concentrations, as detected by Liquid Chromatography-Mass Spectrometry (HPLC-MS/MS) based multimetabolite method. The extract did not show any cytotoxic activity in non-tumor porcine liver primary culture cells and human tumor cell lines. The antibacterial effect on human clinical isolates and antifungal effect on foodborne and environme ntal isolates was also not significant. However, it showed significant effects on seed germination as well as on root and shoot growth of tomato, lettuce, wheat and oat. The root growth inhibition ranged from 65.9% to 100%, and the shoot growth was reduced in 22.7% to 92%, being tomato the most sensitive plant. The extract can be considered as a potential bioherbicide by its phytotoxic effect, without noticeable negative effects on human and animal cells and also on the environment. Further tests on herbicidal potential are now under development.Espécies fúngicas do género Penicillium são fontes promissoras de substâncias bioativas. Estirpes isoladas de habitats pouco caracterizados são atualmente sujeitos a forte investigação como potenciais fontes destas substâncias. Penicillium nordicum é uma espécie reconhecida pela sua capacidade para produzir a micotoxina ocratoxina A em matrizes alimentares ricas em proteína e sal. No entanto, é também produtora de uma alargada gama de outros metabolitos secundários. Num estudo prévio, foi identificada em estirpes desta espécie a capacidade de, sob determinadas condições de incubação, produzir um composto desconhecido em quantidades aparentemente elevadas, sem produzir ocratoxina A. O objetivo do presente estudo foi avaliar as propriedades bioativas deste composto, nomeadamente propriedades antioxidantes, antimicrobianas, citotóxicas e fitotóxicas. Para o efeito, o fungo foi produzido em meio de cultura à base de chouriço e o extrato aquoso foi usado para os testes necessários. Após análise multi- metabolito por Cromatografia Líquida – Espetrometria de Massa (HPLC-MS/MS), pestalotina foi o composto encontrado no extrato em maior quantidade, sendo os compostos questiomicina e 7-hidroxipestalotina encontrados em menores concentrações. O extrato não mostrou atividade citotóxica em cultura células primár ias de fígado de porco não-tumorais ou em linhas celulares humanas tumorais. O efeito antibacteriano em isolados clínicos humanos e antifúngico em isolados de origem alimentar e ambiental não foi significativo. No entanto, foi detetado efeito fitotóxico significativo ao nível da germinação de sementes e do crescimento de raízes e rebentos de tomate, alface, trigo e aveia. A inibição do crescimento radicular variou entre 65.9% e 100%, e o crescimento do rebento foi inibido em 22.7% a 92%. O tomate foi a espécie mais sensível ao extrato. Pelo efeito fitotóxico observado neste estudo, o extrato pode ser considerado um potencial bioherbicida, sem efeitos negativos significativos para células animais e para o ambiente. O potencial bioherbicida do extrato continua em estudo.Finally I would like to thank Instituto Politécnico de Bragança for permission for realisation of this project and also to Fundação para a Ciência e a Tecnologia (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013)Rodrigues, PaulaFerreira, Isabel C.F.R.Biblioteca Digital do IPBWierzbínska, Agata Karolina2017-08-02T09:18:01Z201720172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10198/14396TID:201722267enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:33:51Zoai:bibliotecadigital.ipb.pt:10198/14396Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:04:15.689500Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of bioactive potential of a secondary metabolite produced by Penicillium nordicum
title Evaluation of bioactive potential of a secondary metabolite produced by Penicillium nordicum
spellingShingle Evaluation of bioactive potential of a secondary metabolite produced by Penicillium nordicum
Wierzbínska, Agata Karolina
Penicillium nordicum
Cytotoxicity
Antioxidant
Antimicrobial
Bioherbicide
Domínio/Área Científica::Ciências Agrárias::Biotecnologia Agrária e Alimentar
title_short Evaluation of bioactive potential of a secondary metabolite produced by Penicillium nordicum
title_full Evaluation of bioactive potential of a secondary metabolite produced by Penicillium nordicum
title_fullStr Evaluation of bioactive potential of a secondary metabolite produced by Penicillium nordicum
title_full_unstemmed Evaluation of bioactive potential of a secondary metabolite produced by Penicillium nordicum
title_sort Evaluation of bioactive potential of a secondary metabolite produced by Penicillium nordicum
author Wierzbínska, Agata Karolina
author_facet Wierzbínska, Agata Karolina
author_role author
dc.contributor.none.fl_str_mv Rodrigues, Paula
Ferreira, Isabel C.F.R.
Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Wierzbínska, Agata Karolina
dc.subject.por.fl_str_mv Penicillium nordicum
Cytotoxicity
Antioxidant
Antimicrobial
Bioherbicide
Domínio/Área Científica::Ciências Agrárias::Biotecnologia Agrária e Alimentar
topic Penicillium nordicum
Cytotoxicity
Antioxidant
Antimicrobial
Bioherbicide
Domínio/Área Científica::Ciências Agrárias::Biotecnologia Agrária e Alimentar
description Fungi of the genus Penicillium are promising sources of bioactive substances. Fungal strains isolated from poorly characterized habitats are currently under active investigation as potential producers of biologically active compounds. Penicillium nordicum is mostly known by its ability to produce the mycotoxin ochratoxin A in several protein- and salt-rich food matrices like processed meat and cheese, but it can also produce an array of other secondary metabolites. In a previous study, strains of this species were found to produce, under specific growth conditions, one unidentified compound in apparently big amounts, without producing ochratoxin A. The aim of the present study was to evaluate the bioactive properties of this compound in terms of antioxidant, antimicrobial, cytotoxic and phytotoxic properties. For that purpose, the fungus was grown on chorizo-based medium and the aqueous extract of the compound was used for further tests. Pestalotin was the predominant compound in the fungal extract, with questiomycin and 7-hydroxypestalotin also being found at lower concentrations, as detected by Liquid Chromatography-Mass Spectrometry (HPLC-MS/MS) based multimetabolite method. The extract did not show any cytotoxic activity in non-tumor porcine liver primary culture cells and human tumor cell lines. The antibacterial effect on human clinical isolates and antifungal effect on foodborne and environme ntal isolates was also not significant. However, it showed significant effects on seed germination as well as on root and shoot growth of tomato, lettuce, wheat and oat. The root growth inhibition ranged from 65.9% to 100%, and the shoot growth was reduced in 22.7% to 92%, being tomato the most sensitive plant. The extract can be considered as a potential bioherbicide by its phytotoxic effect, without noticeable negative effects on human and animal cells and also on the environment. Further tests on herbicidal potential are now under development.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-02T09:18:01Z
2017
2017
2017-01-01T00:00:00Z
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