In vivo MRI assessment of a novel GdIII-based contrast agent designed for high magnetic field applications
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/8439 https://doi.org/10.1002/cmmi.233 |
Resumo: | Gd3L is a trinuclear Gd3+ complex of intermediate size, designed for contrast agent applications in high field magnetic resonance imaging (H12L is based on a trimethylbenzene core bearing three methylene-diethylenetriamine- N,N,NPrimeNPrime-tetraacetate moieties). Thanks to its appropriate size, the presence of two inner sphere water molecules and a fast water exchange, Gd3L has remarkable proton relaxivities at high magnetic field (r1 = 10.2 vs 3.0 mM-1 s-1 for GdDOTA at 9.4 T, 37°C, in H2O). Here we report an in vivo MRI feasibility study, complemented with dynamic gamma scintigraphic imaging and biodistribution experiments using the 153Sm-enriched analog. MRI experiments were performed at 9.4 T in mice with Gd3L and the commercial contrast agent gadolinium(III)-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (GdDOTA). Gd3L was well tolerated by the animals at the dose of 8 µmol Gd kg-1 body weight. Dynamic contrast enhanced (DCE) images showed considerably higher signal enhancement in the kidney medulla and cortex after Gd3L injection than after GdDOTA injection at an identical dose. The relaxation rates, DeltaR1, were calculated from the IR TrueFISP data. During the excretory phase, the DeltaR1 for various tissues was similar for Gd3L and GdDOTA, when the latter was injected at a three-fold higher dose (24 vs 8 µmol Gd kg-1 body weight). These results point to an approximately three times higher in vivo relaxivity (per Gd) for Gd3L relative to GdDOTA, thus the ratio of the relaxivities of the two compounds determined in vitro is retained under in vivo conditions. They also indicate that the two inner sphere water molecules per Gd in Gd3L are not substantially replaced by endogenous anions or other donor groups under physiological conditions. Gd3L has a pharmacokinetics typical of small, hydrophilic complexes, involving fast renal clearance and no retention in the blood pool. The dynamic gamma scintigraphic studies and the biodistribution experiments performed in Wistar rats with 153Sm-enriched *Sm3L are also indicative of a fast elimination via the kidneys. Copyright © 2008 John Wiley & Sons, Ltd. |
id |
RCAP_be3501fb544551d826092f76d6e3af34 |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/8439 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
In vivo MRI assessment of a novel GdIII-based contrast agent designed for high magnetic field applicationsGd3L is a trinuclear Gd3+ complex of intermediate size, designed for contrast agent applications in high field magnetic resonance imaging (H12L is based on a trimethylbenzene core bearing three methylene-diethylenetriamine- N,N,NPrimeNPrime-tetraacetate moieties). Thanks to its appropriate size, the presence of two inner sphere water molecules and a fast water exchange, Gd3L has remarkable proton relaxivities at high magnetic field (r1 = 10.2 vs 3.0 mM-1 s-1 for GdDOTA at 9.4 T, 37°C, in H2O). Here we report an in vivo MRI feasibility study, complemented with dynamic gamma scintigraphic imaging and biodistribution experiments using the 153Sm-enriched analog. MRI experiments were performed at 9.4 T in mice with Gd3L and the commercial contrast agent gadolinium(III)-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (GdDOTA). Gd3L was well tolerated by the animals at the dose of 8 µmol Gd kg-1 body weight. Dynamic contrast enhanced (DCE) images showed considerably higher signal enhancement in the kidney medulla and cortex after Gd3L injection than after GdDOTA injection at an identical dose. The relaxation rates, DeltaR1, were calculated from the IR TrueFISP data. During the excretory phase, the DeltaR1 for various tissues was similar for Gd3L and GdDOTA, when the latter was injected at a three-fold higher dose (24 vs 8 µmol Gd kg-1 body weight). These results point to an approximately three times higher in vivo relaxivity (per Gd) for Gd3L relative to GdDOTA, thus the ratio of the relaxivities of the two compounds determined in vitro is retained under in vivo conditions. They also indicate that the two inner sphere water molecules per Gd in Gd3L are not substantially replaced by endogenous anions or other donor groups under physiological conditions. Gd3L has a pharmacokinetics typical of small, hydrophilic complexes, involving fast renal clearance and no retention in the blood pool. The dynamic gamma scintigraphic studies and the biodistribution experiments performed in Wistar rats with 153Sm-enriched *Sm3L are also indicative of a fast elimination via the kidneys. Copyright © 2008 John Wiley & Sons, Ltd.2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/8439http://hdl.handle.net/10316/8439https://doi.org/10.1002/cmmi.233engContrast Media & Molecular Imaging. 3:2 (2008) 78-85Sousa, Paulo Loureiro deLivramento, João BrunoHelm, LotharMerbach, André E.Même, WilliamDoan, Bich-ThuyBeloeil, Jean-ClaudePrata, Maria I. M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-05-25T02:33:52Zoai:estudogeral.uc.pt:10316/8439Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:43:30.278453Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
In vivo MRI assessment of a novel GdIII-based contrast agent designed for high magnetic field applications |
title |
In vivo MRI assessment of a novel GdIII-based contrast agent designed for high magnetic field applications |
spellingShingle |
In vivo MRI assessment of a novel GdIII-based contrast agent designed for high magnetic field applications Sousa, Paulo Loureiro de |
title_short |
In vivo MRI assessment of a novel GdIII-based contrast agent designed for high magnetic field applications |
title_full |
In vivo MRI assessment of a novel GdIII-based contrast agent designed for high magnetic field applications |
title_fullStr |
In vivo MRI assessment of a novel GdIII-based contrast agent designed for high magnetic field applications |
title_full_unstemmed |
In vivo MRI assessment of a novel GdIII-based contrast agent designed for high magnetic field applications |
title_sort |
In vivo MRI assessment of a novel GdIII-based contrast agent designed for high magnetic field applications |
author |
Sousa, Paulo Loureiro de |
author_facet |
Sousa, Paulo Loureiro de Livramento, João Bruno Helm, Lothar Merbach, André E. Même, William Doan, Bich-Thuy Beloeil, Jean-Claude Prata, Maria I. M. |
author_role |
author |
author2 |
Livramento, João Bruno Helm, Lothar Merbach, André E. Même, William Doan, Bich-Thuy Beloeil, Jean-Claude Prata, Maria I. M. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Sousa, Paulo Loureiro de Livramento, João Bruno Helm, Lothar Merbach, André E. Même, William Doan, Bich-Thuy Beloeil, Jean-Claude Prata, Maria I. M. |
description |
Gd3L is a trinuclear Gd3+ complex of intermediate size, designed for contrast agent applications in high field magnetic resonance imaging (H12L is based on a trimethylbenzene core bearing three methylene-diethylenetriamine- N,N,NPrimeNPrime-tetraacetate moieties). Thanks to its appropriate size, the presence of two inner sphere water molecules and a fast water exchange, Gd3L has remarkable proton relaxivities at high magnetic field (r1 = 10.2 vs 3.0 mM-1 s-1 for GdDOTA at 9.4 T, 37°C, in H2O). Here we report an in vivo MRI feasibility study, complemented with dynamic gamma scintigraphic imaging and biodistribution experiments using the 153Sm-enriched analog. MRI experiments were performed at 9.4 T in mice with Gd3L and the commercial contrast agent gadolinium(III)-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (GdDOTA). Gd3L was well tolerated by the animals at the dose of 8 µmol Gd kg-1 body weight. Dynamic contrast enhanced (DCE) images showed considerably higher signal enhancement in the kidney medulla and cortex after Gd3L injection than after GdDOTA injection at an identical dose. The relaxation rates, DeltaR1, were calculated from the IR TrueFISP data. During the excretory phase, the DeltaR1 for various tissues was similar for Gd3L and GdDOTA, when the latter was injected at a three-fold higher dose (24 vs 8 µmol Gd kg-1 body weight). These results point to an approximately three times higher in vivo relaxivity (per Gd) for Gd3L relative to GdDOTA, thus the ratio of the relaxivities of the two compounds determined in vitro is retained under in vivo conditions. They also indicate that the two inner sphere water molecules per Gd in Gd3L are not substantially replaced by endogenous anions or other donor groups under physiological conditions. Gd3L has a pharmacokinetics typical of small, hydrophilic complexes, involving fast renal clearance and no retention in the blood pool. The dynamic gamma scintigraphic studies and the biodistribution experiments performed in Wistar rats with 153Sm-enriched *Sm3L are also indicative of a fast elimination via the kidneys. Copyright © 2008 John Wiley & Sons, Ltd. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/8439 http://hdl.handle.net/10316/8439 https://doi.org/10.1002/cmmi.233 |
url |
http://hdl.handle.net/10316/8439 https://doi.org/10.1002/cmmi.233 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Contrast Media & Molecular Imaging. 3:2 (2008) 78-85 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133707209015296 |