Regucalcin is expressed in rat mammary gland and prostate and down-regulated by 17beta-estradiol

Detalhes bibliográficos
Autor(a) principal: Maia, C J
Data de Publicação: 2008
Outros Autores: Santos, Cecilia, Schmitt, Fernando, Socorro, Sílvia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.6/7218
Resumo: Regucalcin is involved in maintenance of calcium homeostasis due to the activation of Ca2+ pumping enzymes in the plasma membrane. It has a suppressive effect in cell proliferation, DNA and RNA synthesis, and may be associated with the abnormal cell division on tumor tissues. On the other hand both estrogens and Ca2+ are implicated in breast and prostate cancer but there are no studies focused on the expression of regucalcin in rat mammary gland or prostate. Furthermore, it is known that the expression of regucalcin in rat liver and kidney is regulated by 17beta-estradiol (E2). The aim of this study is to analyze if regucalcin is expressed in rat mammary gland and prostate and if it is regulated by E2 in these tissues. We demonstrated for the first time that regucalcin mRNA and protein are present in rat mammary gland and prostate by in situ hybridization and immunohistochemistry, respectively. Furthermore, we show by Real-time PCR that E2 down-regulates regucalcin expression in rat mammary gland and prostate.
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spelling Regucalcin is expressed in rat mammary gland and prostate and down-regulated by 17beta-estradiolRegucalcinSMP30CalciumMammary glandProstate17beta-estradiolRegucalcin is involved in maintenance of calcium homeostasis due to the activation of Ca2+ pumping enzymes in the plasma membrane. It has a suppressive effect in cell proliferation, DNA and RNA synthesis, and may be associated with the abnormal cell division on tumor tissues. On the other hand both estrogens and Ca2+ are implicated in breast and prostate cancer but there are no studies focused on the expression of regucalcin in rat mammary gland or prostate. Furthermore, it is known that the expression of regucalcin in rat liver and kidney is regulated by 17beta-estradiol (E2). The aim of this study is to analyze if regucalcin is expressed in rat mammary gland and prostate and if it is regulated by E2 in these tissues. We demonstrated for the first time that regucalcin mRNA and protein are present in rat mammary gland and prostate by in situ hybridization and immunohistochemistry, respectively. Furthermore, we show by Real-time PCR that E2 down-regulates regucalcin expression in rat mammary gland and prostate.uBibliorumMaia, C JSantos, CeciliaSchmitt, FernandoSocorro, Sílvia2019-10-03T15:30:37Z2008-042008-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/7218eng10.1007/s11010-007-9697-xmetadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-15T09:46:24Zoai:ubibliorum.ubi.pt:10400.6/7218Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:47:46.654463Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Regucalcin is expressed in rat mammary gland and prostate and down-regulated by 17beta-estradiol
title Regucalcin is expressed in rat mammary gland and prostate and down-regulated by 17beta-estradiol
spellingShingle Regucalcin is expressed in rat mammary gland and prostate and down-regulated by 17beta-estradiol
Maia, C J
Regucalcin
SMP30
Calcium
Mammary gland
Prostate
17beta-estradiol
title_short Regucalcin is expressed in rat mammary gland and prostate and down-regulated by 17beta-estradiol
title_full Regucalcin is expressed in rat mammary gland and prostate and down-regulated by 17beta-estradiol
title_fullStr Regucalcin is expressed in rat mammary gland and prostate and down-regulated by 17beta-estradiol
title_full_unstemmed Regucalcin is expressed in rat mammary gland and prostate and down-regulated by 17beta-estradiol
title_sort Regucalcin is expressed in rat mammary gland and prostate and down-regulated by 17beta-estradiol
author Maia, C J
author_facet Maia, C J
Santos, Cecilia
Schmitt, Fernando
Socorro, Sílvia
author_role author
author2 Santos, Cecilia
Schmitt, Fernando
Socorro, Sílvia
author2_role author
author
author
dc.contributor.none.fl_str_mv uBibliorum
dc.contributor.author.fl_str_mv Maia, C J
Santos, Cecilia
Schmitt, Fernando
Socorro, Sílvia
dc.subject.por.fl_str_mv Regucalcin
SMP30
Calcium
Mammary gland
Prostate
17beta-estradiol
topic Regucalcin
SMP30
Calcium
Mammary gland
Prostate
17beta-estradiol
description Regucalcin is involved in maintenance of calcium homeostasis due to the activation of Ca2+ pumping enzymes in the plasma membrane. It has a suppressive effect in cell proliferation, DNA and RNA synthesis, and may be associated with the abnormal cell division on tumor tissues. On the other hand both estrogens and Ca2+ are implicated in breast and prostate cancer but there are no studies focused on the expression of regucalcin in rat mammary gland or prostate. Furthermore, it is known that the expression of regucalcin in rat liver and kidney is regulated by 17beta-estradiol (E2). The aim of this study is to analyze if regucalcin is expressed in rat mammary gland and prostate and if it is regulated by E2 in these tissues. We demonstrated for the first time that regucalcin mRNA and protein are present in rat mammary gland and prostate by in situ hybridization and immunohistochemistry, respectively. Furthermore, we show by Real-time PCR that E2 down-regulates regucalcin expression in rat mammary gland and prostate.
publishDate 2008
dc.date.none.fl_str_mv 2008-04
2008-04-01T00:00:00Z
2019-10-03T15:30:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.6/7218
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dc.language.iso.fl_str_mv eng
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