The chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extract
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/21847 |
Resumo: | Withania chevalieri, endogenous from Cape Verde, is a medicinal plant used in ethnomedicine with a large spectrum of applications, such as treating skin fungal infections caused by dermatophytes. The aim of this work was to chemically characterize the W. chevalieri crude ethanolic extract (WcCEE), and evaluate its bioactivities as antidermatophytic, antioxidant, anti-inflammatory and anticancer, as well as its cytotoxicity. WcCEE was chemically characterized via HPLC-MS. The minimal inhibitory concentration, minimal fungicidal concentration, time-kill and checkerboard assays were used to study the antidermatophytic activity of WcCEE. As an approach to the mechanism of action, the cell wall components, & beta;-1,3-glucan and chitin, and cell membrane ergosterol were quantified. Transmission electron microscopy (TEM) allowed for the study of the fungal ultrastructure. WcCEE contained phenolic acids, flavonoids and terpenes. It had a concentration-dependent fungicidal activity, not inducing relevant resistance, and was endowed with synergistic effects, especially terbinafine. TEM showed severely damaged fungi; the cell membrane and cell wall components levels had slight modifications. The extract had antioxidant, anti-inflammatory and anti-cancer activities, with low toxicity to non-tumoral cell lines. The results demonstrated the potential of WcCEE as an antidermatophytic agent, with antioxidant, anti-inflammatory and anticancer activity, to be safely used in pharmaceutical and dermocosmetic applications. |
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The chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extractWithania chevalieriCape VerdeAntidermatophytic activityAntioxidant activityAnti-inflammatory activityAnticancer activityCytotoxicityWithania chevalieri, endogenous from Cape Verde, is a medicinal plant used in ethnomedicine with a large spectrum of applications, such as treating skin fungal infections caused by dermatophytes. The aim of this work was to chemically characterize the W. chevalieri crude ethanolic extract (WcCEE), and evaluate its bioactivities as antidermatophytic, antioxidant, anti-inflammatory and anticancer, as well as its cytotoxicity. WcCEE was chemically characterized via HPLC-MS. The minimal inhibitory concentration, minimal fungicidal concentration, time-kill and checkerboard assays were used to study the antidermatophytic activity of WcCEE. As an approach to the mechanism of action, the cell wall components, & beta;-1,3-glucan and chitin, and cell membrane ergosterol were quantified. Transmission electron microscopy (TEM) allowed for the study of the fungal ultrastructure. WcCEE contained phenolic acids, flavonoids and terpenes. It had a concentration-dependent fungicidal activity, not inducing relevant resistance, and was endowed with synergistic effects, especially terbinafine. TEM showed severely damaged fungi; the cell membrane and cell wall components levels had slight modifications. The extract had antioxidant, anti-inflammatory and anti-cancer activities, with low toxicity to non-tumoral cell lines. The results demonstrated the potential of WcCEE as an antidermatophytic agent, with antioxidant, anti-inflammatory and anticancer activity, to be safely used in pharmaceutical and dermocosmetic applications.This work was funded by the European Regional Development Fund (ERDF), through the Centro 2020 Regional Operational Programme: project CENTRO-01-0145-FEDER-000012- HealthyAging2020 and CENTRO-01-0145-FEDER-022095: ViraVector; the COMPETE 2020–Operational Programme for Competitiveness and Internationalisation, and the Portuguese national funds via FCT– Fundação para a Ciência e a Tecnologia, I.P.:UIDB/04539/2020 and UIDP/04539/2020 & Fundação para a Ciência e Tecnologia (FCT)—in the scope of the projects UIDB/04539/2020, UIDP/04539/2020. The authors are also thankful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020); national funding by FCT, P.I., through the institutional scientific employment program contract for R.C.C. and L.B. contracts, and through the individual research grant 2020.06297.MDPIBiblioteca Digital do IPBCorreia, Edmilson Emanuel MonteiroFigueirinha, ArturRodrigues, LisaPinela, JoséCalhelha, Ricardo C.Barros, LillianFernandes, ChantalSalgueiro, LígiaGonçalves, Teresa2020-04-28T09:50:48Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/21847engCorreia, Edmilson Emanuel Monteiro; Figueirinha, Artur; Rodrigues, Lisa; Pinela, José; Calhelha, Ricardo C.; Barros, Lillian; Fernandes, Chantal; Salgueiro, Lígia; Gonçalves, Teresa (2023). The chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extract. Plants. eISSN 2223-7747. 12:13, p. 1-2410.3390/plants121325022223-7747info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-06T01:17:35Zoai:bibliotecadigital.ipb.pt:10198/21847Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:41:19.114947Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extract |
title |
The chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extract |
spellingShingle |
The chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extract Correia, Edmilson Emanuel Monteiro Withania chevalieri Cape Verde Antidermatophytic activity Antioxidant activity Anti-inflammatory activity Anticancer activity Cytotoxicity |
title_short |
The chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extract |
title_full |
The chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extract |
title_fullStr |
The chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extract |
title_full_unstemmed |
The chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extract |
title_sort |
The chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extract |
author |
Correia, Edmilson Emanuel Monteiro |
author_facet |
Correia, Edmilson Emanuel Monteiro Figueirinha, Artur Rodrigues, Lisa Pinela, José Calhelha, Ricardo C. Barros, Lillian Fernandes, Chantal Salgueiro, Lígia Gonçalves, Teresa |
author_role |
author |
author2 |
Figueirinha, Artur Rodrigues, Lisa Pinela, José Calhelha, Ricardo C. Barros, Lillian Fernandes, Chantal Salgueiro, Lígia Gonçalves, Teresa |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Correia, Edmilson Emanuel Monteiro Figueirinha, Artur Rodrigues, Lisa Pinela, José Calhelha, Ricardo C. Barros, Lillian Fernandes, Chantal Salgueiro, Lígia Gonçalves, Teresa |
dc.subject.por.fl_str_mv |
Withania chevalieri Cape Verde Antidermatophytic activity Antioxidant activity Anti-inflammatory activity Anticancer activity Cytotoxicity |
topic |
Withania chevalieri Cape Verde Antidermatophytic activity Antioxidant activity Anti-inflammatory activity Anticancer activity Cytotoxicity |
description |
Withania chevalieri, endogenous from Cape Verde, is a medicinal plant used in ethnomedicine with a large spectrum of applications, such as treating skin fungal infections caused by dermatophytes. The aim of this work was to chemically characterize the W. chevalieri crude ethanolic extract (WcCEE), and evaluate its bioactivities as antidermatophytic, antioxidant, anti-inflammatory and anticancer, as well as its cytotoxicity. WcCEE was chemically characterized via HPLC-MS. The minimal inhibitory concentration, minimal fungicidal concentration, time-kill and checkerboard assays were used to study the antidermatophytic activity of WcCEE. As an approach to the mechanism of action, the cell wall components, & beta;-1,3-glucan and chitin, and cell membrane ergosterol were quantified. Transmission electron microscopy (TEM) allowed for the study of the fungal ultrastructure. WcCEE contained phenolic acids, flavonoids and terpenes. It had a concentration-dependent fungicidal activity, not inducing relevant resistance, and was endowed with synergistic effects, especially terbinafine. TEM showed severely damaged fungi; the cell membrane and cell wall components levels had slight modifications. The extract had antioxidant, anti-inflammatory and anti-cancer activities, with low toxicity to non-tumoral cell lines. The results demonstrated the potential of WcCEE as an antidermatophytic agent, with antioxidant, anti-inflammatory and anticancer activity, to be safely used in pharmaceutical and dermocosmetic applications. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-28T09:50:48Z 2023 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/21847 |
url |
http://hdl.handle.net/10198/21847 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Correia, Edmilson Emanuel Monteiro; Figueirinha, Artur; Rodrigues, Lisa; Pinela, José; Calhelha, Ricardo C.; Barros, Lillian; Fernandes, Chantal; Salgueiro, Lígia; Gonçalves, Teresa (2023). The chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extract. Plants. eISSN 2223-7747. 12:13, p. 1-24 10.3390/plants12132502 2223-7747 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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