Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/4184 |
Resumo: | Matrix Gla protein (Mgp) is a secreted vitamin K-dependent extracellular matrix protein and a physiological inhibitor of calcification whose gene structure, amino acid sequence and tissue distribution have been conserved throughout evolution. In the present work, the turbot (Scophthalmus maximus) mgp cDNA was cloned and the sequence of the deduced protein compared to that of other vertebrates. As expected, it was closer to teleosts than to other vertebrate groups but there was a strict conservation of amino-acids thought to be important for protein function. Analysis of mgp gene expression indicated branchial arches as the site with higher levels of expression, followed by heart, vertebra and kidney. These results were confirmed by in situ hybridization with a strong mgp expression in branchial arch chondrocytes. Mgp was found to accumulate in gills where it appeared to be restricted to chondrocytes from branchial filaments, while in vertebrae it was localized in vertebral end plates, in growth zones, in vertebral arches and spines and in notochord cells. In the soft tissues analysed, Mgp was mainly detected in kidney and heart, consistent with previous data and providing further evidence for a role of Mgp as a calcification inhibitor and a modulator of the mineralization process. Our studies provide evidence that turbot, an important new species for aquaculture, is also a useful model to study function and expression of Mgp. |
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Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulationTurbot MgpGene expressionSoft tissue accumulationHeartVertebraeBranchial archesMatrix Gla protein (Mgp) is a secreted vitamin K-dependent extracellular matrix protein and a physiological inhibitor of calcification whose gene structure, amino acid sequence and tissue distribution have been conserved throughout evolution. In the present work, the turbot (Scophthalmus maximus) mgp cDNA was cloned and the sequence of the deduced protein compared to that of other vertebrates. As expected, it was closer to teleosts than to other vertebrate groups but there was a strict conservation of amino-acids thought to be important for protein function. Analysis of mgp gene expression indicated branchial arches as the site with higher levels of expression, followed by heart, vertebra and kidney. These results were confirmed by in situ hybridization with a strong mgp expression in branchial arch chondrocytes. Mgp was found to accumulate in gills where it appeared to be restricted to chondrocytes from branchial filaments, while in vertebrae it was localized in vertebral end plates, in growth zones, in vertebral arches and spines and in notochord cells. In the soft tissues analysed, Mgp was mainly detected in kidney and heart, consistent with previous data and providing further evidence for a role of Mgp as a calcification inhibitor and a modulator of the mineralization process. Our studies provide evidence that turbot, an important new species for aquaculture, is also a useful model to study function and expression of Mgp.ElsevierSapientiaRoberto, Vania PalmaCavaco, S.S B Viegas, CarlaSimes, DOrtiz-Delgado, J. B.Sarasquete, C.Gavaia, Paulo J.Cancela, Leonor2014-06-04T13:28:35Z20092014-05-30T10:04:41Z2009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/4184eng0044-8486AUT: DSI00813; LCA00739;http://dx.doi.org/10.1016/j.aquaculture.2009.06.020info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-29T10:53:35Zoai:sapientia.ualg.pt:10400.1/4184Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-29T10:53:35Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
title |
Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
spellingShingle |
Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation Roberto, Vania Palma Turbot Mgp Gene expression Soft tissue accumulation Heart Vertebrae Branchial arches |
title_short |
Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
title_full |
Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
title_fullStr |
Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
title_full_unstemmed |
Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
title_sort |
Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
author |
Roberto, Vania Palma |
author_facet |
Roberto, Vania Palma Cavaco, S. S B Viegas, Carla Simes, D Ortiz-Delgado, J. B. Sarasquete, C. Gavaia, Paulo J. Cancela, Leonor |
author_role |
author |
author2 |
Cavaco, S. S B Viegas, Carla Simes, D Ortiz-Delgado, J. B. Sarasquete, C. Gavaia, Paulo J. Cancela, Leonor |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Roberto, Vania Palma Cavaco, S. S B Viegas, Carla Simes, D Ortiz-Delgado, J. B. Sarasquete, C. Gavaia, Paulo J. Cancela, Leonor |
dc.subject.por.fl_str_mv |
Turbot Mgp Gene expression Soft tissue accumulation Heart Vertebrae Branchial arches |
topic |
Turbot Mgp Gene expression Soft tissue accumulation Heart Vertebrae Branchial arches |
description |
Matrix Gla protein (Mgp) is a secreted vitamin K-dependent extracellular matrix protein and a physiological inhibitor of calcification whose gene structure, amino acid sequence and tissue distribution have been conserved throughout evolution. In the present work, the turbot (Scophthalmus maximus) mgp cDNA was cloned and the sequence of the deduced protein compared to that of other vertebrates. As expected, it was closer to teleosts than to other vertebrate groups but there was a strict conservation of amino-acids thought to be important for protein function. Analysis of mgp gene expression indicated branchial arches as the site with higher levels of expression, followed by heart, vertebra and kidney. These results were confirmed by in situ hybridization with a strong mgp expression in branchial arch chondrocytes. Mgp was found to accumulate in gills where it appeared to be restricted to chondrocytes from branchial filaments, while in vertebrae it was localized in vertebral end plates, in growth zones, in vertebral arches and spines and in notochord cells. In the soft tissues analysed, Mgp was mainly detected in kidney and heart, consistent with previous data and providing further evidence for a role of Mgp as a calcification inhibitor and a modulator of the mineralization process. Our studies provide evidence that turbot, an important new species for aquaculture, is also a useful model to study function and expression of Mgp. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2009-01-01T00:00:00Z 2014-06-04T13:28:35Z 2014-05-30T10:04:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/4184 |
url |
http://hdl.handle.net/10400.1/4184 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0044-8486 AUT: DSI00813; LCA00739; http://dx.doi.org/10.1016/j.aquaculture.2009.06.020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817549851725922305 |