Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation

Detalhes bibliográficos
Autor(a) principal: Roberto, Vania Palma
Data de Publicação: 2009
Outros Autores: Cavaco, S., S B Viegas, Carla, Simes, D, Ortiz-Delgado, J. B., Sarasquete, C., Gavaia, Paulo J., Cancela, Leonor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/4184
Resumo: Matrix Gla protein (Mgp) is a secreted vitamin K-dependent extracellular matrix protein and a physiological inhibitor of calcification whose gene structure, amino acid sequence and tissue distribution have been conserved throughout evolution. In the present work, the turbot (Scophthalmus maximus) mgp cDNA was cloned and the sequence of the deduced protein compared to that of other vertebrates. As expected, it was closer to teleosts than to other vertebrate groups but there was a strict conservation of amino-acids thought to be important for protein function. Analysis of mgp gene expression indicated branchial arches as the site with higher levels of expression, followed by heart, vertebra and kidney. These results were confirmed by in situ hybridization with a strong mgp expression in branchial arch chondrocytes. Mgp was found to accumulate in gills where it appeared to be restricted to chondrocytes from branchial filaments, while in vertebrae it was localized in vertebral end plates, in growth zones, in vertebral arches and spines and in notochord cells. In the soft tissues analysed, Mgp was mainly detected in kidney and heart, consistent with previous data and providing further evidence for a role of Mgp as a calcification inhibitor and a modulator of the mineralization process. Our studies provide evidence that turbot, an important new species for aquaculture, is also a useful model to study function and expression of Mgp.
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spelling Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulationTurbot MgpGene expressionSoft tissue accumulationHeartVertebraeBranchial archesMatrix Gla protein (Mgp) is a secreted vitamin K-dependent extracellular matrix protein and a physiological inhibitor of calcification whose gene structure, amino acid sequence and tissue distribution have been conserved throughout evolution. In the present work, the turbot (Scophthalmus maximus) mgp cDNA was cloned and the sequence of the deduced protein compared to that of other vertebrates. As expected, it was closer to teleosts than to other vertebrate groups but there was a strict conservation of amino-acids thought to be important for protein function. Analysis of mgp gene expression indicated branchial arches as the site with higher levels of expression, followed by heart, vertebra and kidney. These results were confirmed by in situ hybridization with a strong mgp expression in branchial arch chondrocytes. Mgp was found to accumulate in gills where it appeared to be restricted to chondrocytes from branchial filaments, while in vertebrae it was localized in vertebral end plates, in growth zones, in vertebral arches and spines and in notochord cells. In the soft tissues analysed, Mgp was mainly detected in kidney and heart, consistent with previous data and providing further evidence for a role of Mgp as a calcification inhibitor and a modulator of the mineralization process. Our studies provide evidence that turbot, an important new species for aquaculture, is also a useful model to study function and expression of Mgp.ElsevierSapientiaRoberto, Vania PalmaCavaco, S.S B Viegas, CarlaSimes, DOrtiz-Delgado, J. B.Sarasquete, C.Gavaia, Paulo J.Cancela, Leonor2014-06-04T13:28:35Z20092014-05-30T10:04:41Z2009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/4184eng0044-8486AUT: DSI00813; LCA00739;http://dx.doi.org/10.1016/j.aquaculture.2009.06.020info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-29T10:53:35Zoai:sapientia.ualg.pt:10400.1/4184Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-29T10:53:35Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation
title Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation
spellingShingle Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation
Roberto, Vania Palma
Turbot Mgp
Gene expression
Soft tissue accumulation
Heart
Vertebrae
Branchial arches
title_short Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation
title_full Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation
title_fullStr Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation
title_full_unstemmed Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation
title_sort Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation
author Roberto, Vania Palma
author_facet Roberto, Vania Palma
Cavaco, S.
S B Viegas, Carla
Simes, D
Ortiz-Delgado, J. B.
Sarasquete, C.
Gavaia, Paulo J.
Cancela, Leonor
author_role author
author2 Cavaco, S.
S B Viegas, Carla
Simes, D
Ortiz-Delgado, J. B.
Sarasquete, C.
Gavaia, Paulo J.
Cancela, Leonor
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Roberto, Vania Palma
Cavaco, S.
S B Viegas, Carla
Simes, D
Ortiz-Delgado, J. B.
Sarasquete, C.
Gavaia, Paulo J.
Cancela, Leonor
dc.subject.por.fl_str_mv Turbot Mgp
Gene expression
Soft tissue accumulation
Heart
Vertebrae
Branchial arches
topic Turbot Mgp
Gene expression
Soft tissue accumulation
Heart
Vertebrae
Branchial arches
description Matrix Gla protein (Mgp) is a secreted vitamin K-dependent extracellular matrix protein and a physiological inhibitor of calcification whose gene structure, amino acid sequence and tissue distribution have been conserved throughout evolution. In the present work, the turbot (Scophthalmus maximus) mgp cDNA was cloned and the sequence of the deduced protein compared to that of other vertebrates. As expected, it was closer to teleosts than to other vertebrate groups but there was a strict conservation of amino-acids thought to be important for protein function. Analysis of mgp gene expression indicated branchial arches as the site with higher levels of expression, followed by heart, vertebra and kidney. These results were confirmed by in situ hybridization with a strong mgp expression in branchial arch chondrocytes. Mgp was found to accumulate in gills where it appeared to be restricted to chondrocytes from branchial filaments, while in vertebrae it was localized in vertebral end plates, in growth zones, in vertebral arches and spines and in notochord cells. In the soft tissues analysed, Mgp was mainly detected in kidney and heart, consistent with previous data and providing further evidence for a role of Mgp as a calcification inhibitor and a modulator of the mineralization process. Our studies provide evidence that turbot, an important new species for aquaculture, is also a useful model to study function and expression of Mgp.
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
2014-06-04T13:28:35Z
2014-05-30T10:04:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/4184
url http://hdl.handle.net/10400.1/4184
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0044-8486
AUT: DSI00813; LCA00739;
http://dx.doi.org/10.1016/j.aquaculture.2009.06.020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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