Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization

Detalhes bibliográficos
Autor(a) principal: Bonet, Aina
Data de Publicação: 2021
Outros Autores: Valença, Andreia, Mendes-Jorge, Luísa, Casellas, Alba, Rodríguez-Baeza, Alfonso, Nacher, Víctor, Ramos, David, Pampalona, Judit, Simo, Rafael, Ruberte, Jesús
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/23248
Resumo: Research Areas: Ophthalmology
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spelling Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularizationEndostatinIntravitreal vesselsDiabetic retinopathydb/db miceResearch Areas: OphthalmologyABSTRACT - Endostatin, a naturally cleaved fragment of type XVIII collagen with antiangiogenic activity, has been involved in the regulation of neovascularization during diabetic retinopathy. Here, the intracellular distribution of endostatin in healthy mouse and human neuroretinas has been analyzed. In addition, to study the effect of experimental hyperglycemia on retinal endostatin, the db/db mouse model has been used. Endostatin protein expression in mouse and human retinas was studied by immunofluorescence and Western blot, and compared with db/db mice. Eye fundus angiography, histology, and immunofluorescence were used to visualize mouse retinal and intravitreal vessels. For the first time, our results revealed the presence of endostatin in neurons of mouse and human retinas. Endostatin was mainly expressed in bipolar cells and photoreceptors, in contrast to the optic disc, where endostatin expression was undetectable. Diabetic mice showed a reduction of endostatin in their retinas associated with the appearance of intravitreal vessels at the optic disc in 50% of db/db mice. Intravitreal vessels showed GFAP positive neuroglia sheath, basement membrane thickening by collagen IV deposition, and presence of MMP-2 and MMP-9 in the vascular wall. All together, these results point that decreased retinal endostatin during experimental diabetes is associated with optic disc intravitreal vascularization. Based on their phenotype, these intravitreal vessels could be neovessels. However, it cannot be ruled out the possibility that they may also represent persistent hyaloid vessels.WileyRepositório da Universidade de LisboaBonet, AinaValença, AndreiaMendes-Jorge, LuísaCasellas, AlbaRodríguez-Baeza, AlfonsoNacher, VíctorRamos, DavidPampalona, JuditSimo, RafaelRuberte, Jesús2022-01-24T23:58:36Z2021-10-212021-10-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23248engBonet A, Valenta A, Mendes-Jorge L, Casellas A, Rodriguez-Baeza A, Nacher V, Ramos D, Pampalona J, Simo R, Ruberte J. 2021. Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization. Experimental Eye Research. 212:108801 . Doi: 10.1016/j.exer.2021.1088010014-483510.1016/j.exer.2021.1088011096-0007info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:45Zoai:www.repository.utl.pt:10400.5/23248Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:31.341775Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization
title Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization
spellingShingle Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization
Bonet, Aina
Endostatin
Intravitreal vessels
Diabetic retinopathy
db/db mice
title_short Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization
title_full Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization
title_fullStr Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization
title_full_unstemmed Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization
title_sort Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization
author Bonet, Aina
author_facet Bonet, Aina
Valença, Andreia
Mendes-Jorge, Luísa
Casellas, Alba
Rodríguez-Baeza, Alfonso
Nacher, Víctor
Ramos, David
Pampalona, Judit
Simo, Rafael
Ruberte, Jesús
author_role author
author2 Valença, Andreia
Mendes-Jorge, Luísa
Casellas, Alba
Rodríguez-Baeza, Alfonso
Nacher, Víctor
Ramos, David
Pampalona, Judit
Simo, Rafael
Ruberte, Jesús
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Bonet, Aina
Valença, Andreia
Mendes-Jorge, Luísa
Casellas, Alba
Rodríguez-Baeza, Alfonso
Nacher, Víctor
Ramos, David
Pampalona, Judit
Simo, Rafael
Ruberte, Jesús
dc.subject.por.fl_str_mv Endostatin
Intravitreal vessels
Diabetic retinopathy
db/db mice
topic Endostatin
Intravitreal vessels
Diabetic retinopathy
db/db mice
description Research Areas: Ophthalmology
publishDate 2021
dc.date.none.fl_str_mv 2021-10-21
2021-10-21T00:00:00Z
2022-01-24T23:58:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/23248
url http://hdl.handle.net/10400.5/23248
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bonet A, Valenta A, Mendes-Jorge L, Casellas A, Rodriguez-Baeza A, Nacher V, Ramos D, Pampalona J, Simo R, Ruberte J. 2021. Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization. Experimental Eye Research. 212:108801 . Doi: 10.1016/j.exer.2021.108801
0014-4835
10.1016/j.exer.2021.108801
1096-0007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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