Nasal colonization by Staphylococcus aureusin Health Sciences students and analysis of risk fac-tors under a One Health perspective

• Autor(a) principal: Gomes-Sampaio, L. M.
• Data de Publicação: 2023
• Outros Autores: Cláudia-Ferreira, A., Prata, J. C., Azevedo, R. M. S., Pacheco, P., Campos, Carla, Novais, C., Peixe, L., Dinis-Oliveira, R. J., Coelho, C., Miranda, C., Quinteira, S., Freitas, A. R.
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.22/25779
• topic: Staphylococcus aureus; MRSA; University students; One Health

Staphylococcus aureusis the leading bacterial cause of death globally. Nasal carriage of S. aureusincreases the risk of invasive infections, including by methicillin-resistant S. aureus(MRSA) strains, but studies including Portuguese university students (PUS) are scarce. To analyse the prevalence of methicillin-susceptible S. aureus(MSSA) and MRSA among PUS enrolled in different courses/years (1st-4th) at IUCS-CESPU, characterize their antibiotic resistance profiles, and assess the potential risk factors. Swabs collected during March-December 2022 from anterior nares of 156 volunteers (median 22-years) were processed in mannitol-salt agar and, in parallel, enriched in brain-heart broth with NaCl 6.5% further plated onto ChromID® MRSA SMART. Typical colonies were stored for species identification (MALDITOF-MS) and antibiotic susceptibility testing (disk diffusion; EU-CAST/CLSI guidelines). Each student completed a questionnaire comprising demographic/clinical/social parameters. Statistical analysis was conducted in IBM-SPSS Statistics 26 using binary logistic regression applying a backward stepwise (likelihood ratio) method, with α=0.05, selecting variables using Chi-square tests and Mann-Whitney U tests for which p≤0.20, >10 occurrences, not biologicallycorrelated. Prevalence of MSSA and MRSA (cefoxitin screening) were 28.8% and 1.9%, respectively. From the 45 positive samples, 9% were multidrug-resistant, 38% were resistant to penicillin, 40% to erythromycin, 40% to clindamycin (inducible),7% to cefoxitin, 2% to tetracycline, and 2% to rifampicin. Self-reported frequent contact with animals (OR=3.44, CI 95%: 1.10–10.66) were positively associated with S. aureus, while regular sports participation presented a negative association (OR=0.36, CI 95%: 0.17–0.77). Sports participation was not correlated with self-reported excellent health (χ2=0.680, p=0.409). This is one of the first studies assessing MSSA/MRSA rates in PUS after the COVID-19 pandemics imposing higher self-protection/hygienization. While PUS-MSSA rates are similar to that previously observed, PUS-MRSA rates are slightly higher. Additional samples are being processed to explore future trends and other potential One Health factors influencing MSSA/MRSA colonization.