The role of bacteria in pine wilt disease: insights from microbiome analysis.
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/25331 https://doi.org/Alves, M., A. Pereira, C. Vicente, P. Matos, J. Henriques, H. Lopes, F. Nascimento, M. Mota, A. Correia, and I. Henriques. 2018. The role of bacteria in pine wilt disease: insights from microbiome analysis. FEMS Microbiology Ecology, 94 (7): 1-13, 1 July 2018, fiy077, https://doi.org/10.1093/femsec/fiy077 https://doi.org/doi.org/10.1093/femsec/fiy077 |
Resumo: | Pine Wilt Disease (PWD) has a significant impact on Eurasia pine forests. The microbiome of the nematode (the primary cause of the disease), its insect vector, and the host tree may be relevant for the disease mechanism. The aim of this study was to characterize these microbiomes, from three PWD-affected areas in Portugal, using Denaturing Gradient Gel Electrophoresis, 16S rRNA gene pyrosequencing, and a functional inference-based approach (PICRUSt). The bacterial community structure of the nematode was significantly different from the infected trees but closely related to the insect vector, supporting the hypothesis that the nematode microbiome might be in part inherited from the insect. Sampling location influenced mostly the tree microbiome (P < 0.05). Genes related both with plant growth promotion and phytopathogenicity were predicted for the tree microbiome. Xenobiotic degradation functions were predicted in the nematode and insect microbiomes. Phytotoxin biosynthesis was also predicted for the nematode microbiome, supporting the theory of a direct contribution of the microbiome to tree-wilting. This is the first study that simultaneously characterized the nematode, tree and insect-vector microbiomes from the same affected areas, and overall the results support the hypothesis that the PWD microbiome plays an important role in the disease's development. |
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The role of bacteria in pine wilt disease: insights from microbiome analysis.microbiomenext-generation sequencingpine wilt diseaseBursaphelenchus xylophilusPinus pinasterMonochamus galloprovincialisPine Wilt Disease (PWD) has a significant impact on Eurasia pine forests. The microbiome of the nematode (the primary cause of the disease), its insect vector, and the host tree may be relevant for the disease mechanism. The aim of this study was to characterize these microbiomes, from three PWD-affected areas in Portugal, using Denaturing Gradient Gel Electrophoresis, 16S rRNA gene pyrosequencing, and a functional inference-based approach (PICRUSt). The bacterial community structure of the nematode was significantly different from the infected trees but closely related to the insect vector, supporting the hypothesis that the nematode microbiome might be in part inherited from the insect. Sampling location influenced mostly the tree microbiome (P < 0.05). Genes related both with plant growth promotion and phytopathogenicity were predicted for the tree microbiome. Xenobiotic degradation functions were predicted in the nematode and insect microbiomes. Phytotoxin biosynthesis was also predicted for the nematode microbiome, supporting the theory of a direct contribution of the microbiome to tree-wilting. This is the first study that simultaneously characterized the nematode, tree and insect-vector microbiomes from the same affected areas, and overall the results support the hypothesis that the PWD microbiome plays an important role in the disease's development.Oxford Academic2019-03-01T11:41:20Z2019-03-012018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/25331https://doi.org/Alves, M., A. Pereira, C. Vicente, P. Matos, J. Henriques, H. Lopes, F. Nascimento, M. Mota, A. Correia, and I. Henriques. 2018. The role of bacteria in pine wilt disease: insights from microbiome analysis. FEMS Microbiology Ecology, 94 (7): 1-13, 1 July 2018, fiy077, https://doi.org/10.1093/femsec/fiy077http://hdl.handle.net/10174/25331https://doi.org/doi.org/10.1093/femsec/fiy077engmartasalgueiroalves@gmail.comihenriques@ua.ptndcvicente@uevora.ptmmota@uevora.ptndndndndnd581Alves, MartaHenriques, IsabelPereira, AVicente, CláudiaMota, ManuelMatos, PHenriques, JLopes, HNascimento, FCorreia, Ainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:19:00Zoai:dspace.uevora.pt:10174/25331Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:15:47.120408Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The role of bacteria in pine wilt disease: insights from microbiome analysis. |
title |
The role of bacteria in pine wilt disease: insights from microbiome analysis. |
spellingShingle |
The role of bacteria in pine wilt disease: insights from microbiome analysis. Alves, Marta microbiome next-generation sequencing pine wilt disease Bursaphelenchus xylophilus Pinus pinaster Monochamus galloprovincialis |
title_short |
The role of bacteria in pine wilt disease: insights from microbiome analysis. |
title_full |
The role of bacteria in pine wilt disease: insights from microbiome analysis. |
title_fullStr |
The role of bacteria in pine wilt disease: insights from microbiome analysis. |
title_full_unstemmed |
The role of bacteria in pine wilt disease: insights from microbiome analysis. |
title_sort |
The role of bacteria in pine wilt disease: insights from microbiome analysis. |
author |
Alves, Marta |
author_facet |
Alves, Marta Henriques, Isabel Pereira, A Vicente, Cláudia Mota, Manuel Matos, P Henriques, J Lopes, H Nascimento, F Correia, A |
author_role |
author |
author2 |
Henriques, Isabel Pereira, A Vicente, Cláudia Mota, Manuel Matos, P Henriques, J Lopes, H Nascimento, F Correia, A |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Alves, Marta Henriques, Isabel Pereira, A Vicente, Cláudia Mota, Manuel Matos, P Henriques, J Lopes, H Nascimento, F Correia, A |
dc.subject.por.fl_str_mv |
microbiome next-generation sequencing pine wilt disease Bursaphelenchus xylophilus Pinus pinaster Monochamus galloprovincialis |
topic |
microbiome next-generation sequencing pine wilt disease Bursaphelenchus xylophilus Pinus pinaster Monochamus galloprovincialis |
description |
Pine Wilt Disease (PWD) has a significant impact on Eurasia pine forests. The microbiome of the nematode (the primary cause of the disease), its insect vector, and the host tree may be relevant for the disease mechanism. The aim of this study was to characterize these microbiomes, from three PWD-affected areas in Portugal, using Denaturing Gradient Gel Electrophoresis, 16S rRNA gene pyrosequencing, and a functional inference-based approach (PICRUSt). The bacterial community structure of the nematode was significantly different from the infected trees but closely related to the insect vector, supporting the hypothesis that the nematode microbiome might be in part inherited from the insect. Sampling location influenced mostly the tree microbiome (P < 0.05). Genes related both with plant growth promotion and phytopathogenicity were predicted for the tree microbiome. Xenobiotic degradation functions were predicted in the nematode and insect microbiomes. Phytotoxin biosynthesis was also predicted for the nematode microbiome, supporting the theory of a direct contribution of the microbiome to tree-wilting. This is the first study that simultaneously characterized the nematode, tree and insect-vector microbiomes from the same affected areas, and overall the results support the hypothesis that the PWD microbiome plays an important role in the disease's development. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01T00:00:00Z 2019-03-01T11:41:20Z 2019-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/25331 https://doi.org/Alves, M., A. Pereira, C. Vicente, P. Matos, J. Henriques, H. Lopes, F. Nascimento, M. Mota, A. Correia, and I. Henriques. 2018. The role of bacteria in pine wilt disease: insights from microbiome analysis. FEMS Microbiology Ecology, 94 (7): 1-13, 1 July 2018, fiy077, https://doi.org/10.1093/femsec/fiy077 http://hdl.handle.net/10174/25331 https://doi.org/doi.org/10.1093/femsec/fiy077 |
url |
http://hdl.handle.net/10174/25331 https://doi.org/Alves, M., A. Pereira, C. Vicente, P. Matos, J. Henriques, H. Lopes, F. Nascimento, M. Mota, A. Correia, and I. Henriques. 2018. The role of bacteria in pine wilt disease: insights from microbiome analysis. FEMS Microbiology Ecology, 94 (7): 1-13, 1 July 2018, fiy077, https://doi.org/10.1093/femsec/fiy077 https://doi.org/doi.org/10.1093/femsec/fiy077 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
martasalgueiroalves@gmail.com ihenriques@ua.pt nd cvicente@uevora.pt mmota@uevora.pt nd nd nd nd nd 581 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Oxford Academic |
publisher.none.fl_str_mv |
Oxford Academic |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136639955501056 |