Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV

Detalhes bibliográficos
Autor(a) principal: Ferin, Rita
Data de Publicação: 2013
Outros Autores: Pavão, Maria Leonor, Baptista, José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.3/2747
Resumo: Copyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
id RCAP_c07e6458b4e58b3f45acebf8c6faf32a
oai_identifier_str oai:repositorio.uac.pt:10400.3/2747
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UVAscorbic AcidUric AcidPlasma AntioxidantsIon-exclusionAtherosclerosisHPLC-UVCopyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.OBJECTIVES: Ascorbic (AA) and uric (UA) acids act as antioxidants and are capable to react with biologically relevant oxidants. We aimed to developed a simple, rapid, sensitive, and accurate ion-exclusion HPLC-UV methodology for the simultaneously determination of AA and UA in human plasma. METHODS: Analytical pre-requisites, such as the use of heparin as an anticoagulant and meta-phosphoric acid as a stabilizer were added for accurate and reliable measurements. Chromatographic separation was achieved by an isocratic elution on a HEMA-BIO 1000 SB analytical column using a phosphate buffer, pH 2.4, as a mobile phase. RESULTS: Results indicated an excellent linearity with correlation coefficients (r²) ≥ 0.999. The LOD of AA and UA was 1.02 and 1.42 nmol/mL, respectively, while LOQ ranged from 0.306 to 0.426 nmol/mL. A great repeatability for both antioxidants was found, where the CV (%) values for intra-day were lower than 1.8% and under 6.5% for the inter-day assay. The recovery of AA ranged from 92% to 96% and from 99% to 100% for UA. CONCLUSION: This validated method allows the determination of both antioxidants within 10 min, and is well suited to routine measurements and/or high-throughput clinical analysis. The methodology was applied to assess the antioxidant status of a group of Azorean subjects.Governo Regional dos Açores.ElsevierRepositório da Universidade dos AçoresFerin, RitaPavão, Maria LeonorBaptista, José2014-02-11T15:34:28Z2013-052013-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.3/2747engFERIN, R., PAVÃO, M.L. e BAPTISTA, J. (2013). "Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV", Clinical Biochemistry, 46(7-8): 665-669. http://dx.doi.org/10.1016/j.clinbiochem.2013.01.006.0009-9120 (Print)info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-12-20T14:30:36Zoai:repositorio.uac.pt:10400.3/2747Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:25:24.549514Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV
title Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV
spellingShingle Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV
Ferin, Rita
Ascorbic Acid
Uric Acid
Plasma Antioxidants
Ion-exclusion
Atherosclerosis
HPLC-UV
title_short Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV
title_full Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV
title_fullStr Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV
title_full_unstemmed Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV
title_sort Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV
author Ferin, Rita
author_facet Ferin, Rita
Pavão, Maria Leonor
Baptista, José
author_role author
author2 Pavão, Maria Leonor
Baptista, José
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade dos Açores
dc.contributor.author.fl_str_mv Ferin, Rita
Pavão, Maria Leonor
Baptista, José
dc.subject.por.fl_str_mv Ascorbic Acid
Uric Acid
Plasma Antioxidants
Ion-exclusion
Atherosclerosis
HPLC-UV
topic Ascorbic Acid
Uric Acid
Plasma Antioxidants
Ion-exclusion
Atherosclerosis
HPLC-UV
description Copyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
2013-05-01T00:00:00Z
2014-02-11T15:34:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.3/2747
url http://hdl.handle.net/10400.3/2747
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv FERIN, R., PAVÃO, M.L. e BAPTISTA, J. (2013). "Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV", Clinical Biochemistry, 46(7-8): 665-669. http://dx.doi.org/10.1016/j.clinbiochem.2013.01.006.
0009-9120 (Print)
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799130703682600960

Registros relacionados