Surface modification of bacterial cellulose by nitrogen-containing plasma for improved interaction with cells

Detalhes bibliográficos
Autor(a) principal: Pértile, Renata Aparecida Nedel
Data de Publicação: 2010
Outros Autores: Andrade, Fábia K., Alves Junior, Clodomiro, Gama, F. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/11436
Resumo: Bacterial cellulose (BC) membranes were modified with nitrogen plasma in order to enhance cell affinity. The surface properties of the untreated and plasma modified BC (BCP) were analyzed through contact angle measurements, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). The effect of the plasma treatment on the adhesion of microvascular (HMEC-1), neuroblast (N1E-115) and fibroblast (3T3) cell lines was analyzed. The nitrogen plasma treatment did not increase the wettability of the material, but increased the porosity and surface chemistry, as noticed by the presence of nitrogen. XPS analysis revealed the stability of the modified material along time and autoclave sterilization. The cell adhesion and proliferation of HMEC-1 and N1E-115 cells was significantly improved in the BCP, in contrast with the 3T3 cells, revealing a cell-specific effect. This work highlights the potential of plasma treatment for the modification of the BC surface properties, enhancing its potential for biomedical applications.
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spelling Surface modification of bacterial cellulose by nitrogen-containing plasma for improved interaction with cellsBacterial celluloseNitrogen plasma treatmentX-ray photoelectron spectroscopyCell adhesionScience & TechnologyBacterial cellulose (BC) membranes were modified with nitrogen plasma in order to enhance cell affinity. The surface properties of the untreated and plasma modified BC (BCP) were analyzed through contact angle measurements, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). The effect of the plasma treatment on the adhesion of microvascular (HMEC-1), neuroblast (N1E-115) and fibroblast (3T3) cell lines was analyzed. The nitrogen plasma treatment did not increase the wettability of the material, but increased the porosity and surface chemistry, as noticed by the presence of nitrogen. XPS analysis revealed the stability of the modified material along time and autoclave sterilization. The cell adhesion and proliferation of HMEC-1 and N1E-115 cells was significantly improved in the BCP, in contrast with the 3T3 cells, revealing a cell-specific effect. This work highlights the potential of plasma treatment for the modification of the BC surface properties, enhancing its potential for biomedical applications.Programme Alβan, the European Union Programme of High Level Scholarships for Latin America.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brasil.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brasil.Fundação para a Ciência e a Tecnologia (FCT) – POCTI, Portugal.ElsevierUniversidade do MinhoPértile, Renata Aparecida NedelAndrade, Fábia K.Alves Junior, ClodomiroGama, F. M.20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/11436eng"Carbohydrate Polymers". ISSN 0144-8617. 82:3 (2010) 692–698.0144-861710.1016/j.carbpol.2010.05.037info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:06:51Zoai:repositorium.sdum.uminho.pt:1822/11436Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:57:39.653591Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Surface modification of bacterial cellulose by nitrogen-containing plasma for improved interaction with cells
title Surface modification of bacterial cellulose by nitrogen-containing plasma for improved interaction with cells
spellingShingle Surface modification of bacterial cellulose by nitrogen-containing plasma for improved interaction with cells
Pértile, Renata Aparecida Nedel
Bacterial cellulose
Nitrogen plasma treatment
X-ray photoelectron spectroscopy
Cell adhesion
Science & Technology
title_short Surface modification of bacterial cellulose by nitrogen-containing plasma for improved interaction with cells
title_full Surface modification of bacterial cellulose by nitrogen-containing plasma for improved interaction with cells
title_fullStr Surface modification of bacterial cellulose by nitrogen-containing plasma for improved interaction with cells
title_full_unstemmed Surface modification of bacterial cellulose by nitrogen-containing plasma for improved interaction with cells
title_sort Surface modification of bacterial cellulose by nitrogen-containing plasma for improved interaction with cells
author Pértile, Renata Aparecida Nedel
author_facet Pértile, Renata Aparecida Nedel
Andrade, Fábia K.
Alves Junior, Clodomiro
Gama, F. M.
author_role author
author2 Andrade, Fábia K.
Alves Junior, Clodomiro
Gama, F. M.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pértile, Renata Aparecida Nedel
Andrade, Fábia K.
Alves Junior, Clodomiro
Gama, F. M.
dc.subject.por.fl_str_mv Bacterial cellulose
Nitrogen plasma treatment
X-ray photoelectron spectroscopy
Cell adhesion
Science & Technology
topic Bacterial cellulose
Nitrogen plasma treatment
X-ray photoelectron spectroscopy
Cell adhesion
Science & Technology
description Bacterial cellulose (BC) membranes were modified with nitrogen plasma in order to enhance cell affinity. The surface properties of the untreated and plasma modified BC (BCP) were analyzed through contact angle measurements, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). The effect of the plasma treatment on the adhesion of microvascular (HMEC-1), neuroblast (N1E-115) and fibroblast (3T3) cell lines was analyzed. The nitrogen plasma treatment did not increase the wettability of the material, but increased the porosity and surface chemistry, as noticed by the presence of nitrogen. XPS analysis revealed the stability of the modified material along time and autoclave sterilization. The cell adhesion and proliferation of HMEC-1 and N1E-115 cells was significantly improved in the BCP, in contrast with the 3T3 cells, revealing a cell-specific effect. This work highlights the potential of plasma treatment for the modification of the BC surface properties, enhancing its potential for biomedical applications.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/11436
url http://hdl.handle.net/1822/11436
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Carbohydrate Polymers". ISSN 0144-8617. 82:3 (2010) 692–698.
0144-8617
10.1016/j.carbpol.2010.05.037
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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