Proteomic analysis of chromophobe renal cell carcinoma and benign renal oncocytoma biopsies reveals shared metabolic dysregulation

Detalhes bibliográficos
Autor(a) principal: Carvalho, Luís B.
Data de Publicação: 2023
Outros Autores: Jorge, Susana, López-Fernández, Hugo, Lodeiro, Carlos, Dhir, Rajiv, Campos Pinheiro, Luís, Medeiros, Mariana, Santos, Hugo M., Capelo, José L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/162410
Resumo: PM003/2016. Publisher Copyright: © 2023, The Author(s).
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spelling Proteomic analysis of chromophobe renal cell carcinoma and benign renal oncocytoma biopsies reveals shared metabolic dysregulationChromophobe Renal Cell CarcinomaLabel-free QuantificationMass SpectrometryOCT-embedded tissuesRenal OncocytomaTotal Protein ApproachMolecular MedicineMolecular BiologyClinical BiochemistrySDG 3 - Good Health and Well-beingPM003/2016. Publisher Copyright: © 2023, The Author(s).Background: This study investigates the proteomic landscapes of chromophobe renal cell carcinoma (chRCC) and renal oncocytomas (RO), two subtypes of renal cell carcinoma that together account for approximately 10% of all renal tumors. Despite their histological similarities and shared origins, chRCC is a malignant tumor necessitating aggressive intervention, while RO, a benign growth, is often subject to overtreatment due to difficulties in accurate differentiation. Methods: We conducted a label-free quantitative proteomic analysis on solid biopsies of chRCC (n = 5), RO (n = 5), and normal adjacent tissue (NAT, n = 5). The quantitative analysis was carried out by comparing protein abundances between tumor and NAT specimens. Our analysis identified a total of 1610 proteins across all samples, with 1379 (85.7%) of these proteins quantified in at least seven out of ten LC‒MS/MS runs for one renal tissue type (chRCC, RO, or NAT). Results: Our findings revealed significant similarities in the dysregulation of key metabolic pathways, including carbohydrate, lipid, and amino acid metabolism, in both chRCC and RO. Compared to NAT, both chRCC and RO showed a marked downregulation in gluconeogenesis proteins, but a significant upregulation of proteins integral to the citrate cycle. Interestingly, we observed a distinct divergence in the oxidative phosphorylation pathway, with RO showing a significant increase in the number and degree of alterations in proteins, surpassing that observed in chRCC. Conclusions: This study underscores the value of integrating high-resolution mass spectrometry protein quantification to effectively characterize and differentiate the proteomic landscapes of solid tumor biopsies diagnosed as chRCC and RO. The insights gained from this research offer valuable information for enhancing our understanding of these conditions and may aid in the development of improved diagnostic and therapeutic strategies.DQ - Departamento de QuímicaLAQV@REQUIMTENOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNCarvalho, Luís B.Jorge, SusanaLópez-Fernández, HugoLodeiro, CarlosDhir, RajivCampos Pinheiro, LuísMedeiros, MarianaSantos, Hugo M.Capelo, José L.2024-01-17T22:31:19Z2023-11-282023-11-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/10362/162410eng1542-6416PURE: 81064342https://doi.org/10.1186/s12014-023-09443-8info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-18T01:43:48Zoai:run.unl.pt:10362/162410Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:58:53.495611Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Proteomic analysis of chromophobe renal cell carcinoma and benign renal oncocytoma biopsies reveals shared metabolic dysregulation
title Proteomic analysis of chromophobe renal cell carcinoma and benign renal oncocytoma biopsies reveals shared metabolic dysregulation
spellingShingle Proteomic analysis of chromophobe renal cell carcinoma and benign renal oncocytoma biopsies reveals shared metabolic dysregulation
Carvalho, Luís B.
Chromophobe Renal Cell Carcinoma
Label-free Quantification
Mass Spectrometry
OCT-embedded tissues
Renal Oncocytoma
Total Protein Approach
Molecular Medicine
Molecular Biology
Clinical Biochemistry
SDG 3 - Good Health and Well-being
title_short Proteomic analysis of chromophobe renal cell carcinoma and benign renal oncocytoma biopsies reveals shared metabolic dysregulation
title_full Proteomic analysis of chromophobe renal cell carcinoma and benign renal oncocytoma biopsies reveals shared metabolic dysregulation
title_fullStr Proteomic analysis of chromophobe renal cell carcinoma and benign renal oncocytoma biopsies reveals shared metabolic dysregulation
title_full_unstemmed Proteomic analysis of chromophobe renal cell carcinoma and benign renal oncocytoma biopsies reveals shared metabolic dysregulation
title_sort Proteomic analysis of chromophobe renal cell carcinoma and benign renal oncocytoma biopsies reveals shared metabolic dysregulation
author Carvalho, Luís B.
author_facet Carvalho, Luís B.
Jorge, Susana
López-Fernández, Hugo
Lodeiro, Carlos
Dhir, Rajiv
Campos Pinheiro, Luís
Medeiros, Mariana
Santos, Hugo M.
Capelo, José L.
author_role author
author2 Jorge, Susana
López-Fernández, Hugo
Lodeiro, Carlos
Dhir, Rajiv
Campos Pinheiro, Luís
Medeiros, Mariana
Santos, Hugo M.
Capelo, José L.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
LAQV@REQUIMTE
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Carvalho, Luís B.
Jorge, Susana
López-Fernández, Hugo
Lodeiro, Carlos
Dhir, Rajiv
Campos Pinheiro, Luís
Medeiros, Mariana
Santos, Hugo M.
Capelo, José L.
dc.subject.por.fl_str_mv Chromophobe Renal Cell Carcinoma
Label-free Quantification
Mass Spectrometry
OCT-embedded tissues
Renal Oncocytoma
Total Protein Approach
Molecular Medicine
Molecular Biology
Clinical Biochemistry
SDG 3 - Good Health and Well-being
topic Chromophobe Renal Cell Carcinoma
Label-free Quantification
Mass Spectrometry
OCT-embedded tissues
Renal Oncocytoma
Total Protein Approach
Molecular Medicine
Molecular Biology
Clinical Biochemistry
SDG 3 - Good Health and Well-being
description PM003/2016. Publisher Copyright: © 2023, The Author(s).
publishDate 2023
dc.date.none.fl_str_mv 2023-11-28
2023-11-28T00:00:00Z
2024-01-17T22:31:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/162410
url http://hdl.handle.net/10362/162410
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1542-6416
PURE: 81064342
https://doi.org/10.1186/s12014-023-09443-8
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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