Role of the extracellular matrix in variations of invasive pathways in lung cancers

Detalhes bibliográficos
Autor(a) principal: de Sá, V. K.
Data de Publicação: 2013
Outros Autores: Carvalho, L., Gomes, A., Alarcão, A., Silva, M. R., Couceiro, P., Sousa, V., Soares, F. A., Capelozzi, V. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/109719
https://doi.org/10.1590/1414-431x20122263
Resumo: Among the most common features of highly invasive tumors, such as lung adenocarcinomas (AD) and squamous cell carcinomas (SqCC), is the massive degradation of the extracellular matrix. The remarkable qualitative and quantitative modifications of hyaluronidases (HAases), hyaluronan synthases (HAS), E-cadherin adhesion molecules, and the transforming growth factor β (TGF-β) may favor invasion, cellular motility, and proliferation. We examined HAase proteins (Hyal), HAS, E-cadherin, and TGF-β profiles in lung AD subtypes and SqCC obtained from smokers and non-smokers. Fifty-six patients, median age 64 years, who underwent lobectomy for AD (N = 31) and SqCC (N = 25) were included in the study. HAS-1, -2 and -3, and Hyal-1 and -3 were significantly more expressed by tumor cells than normal and stroma cells (P < 0.01). When stratified according to histologic types, HAS-3 and Hyal-1 immunoreactivity was significantly increased in tumor cells of AD (P = 0.01) and stroma of SqCC (P = 0.002), respectively. Tobacco history in patients with AD was significantly associated with increased HAS-3 immunoreactivity in tumor cells (P < 0.01). Stroma cells of SqCC from non-smokers presented a significant association with HAS-3 (P < 0.01). Hyal, HAS, E-cadherin, and TGF-β modulate a different tumor-induced invasive pathway in lung AD subgroups and SqCC. HAases in resected AD and SqCC were strongly related to the prognosis. Therefore, our findings suggest that strategies aimed at preventing high HAS-3 and Hyal-1 synthesis, or local responses to low TGF-β and E-cadherin, may have a greater impact in lung cancer prognosis.
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spelling Role of the extracellular matrix in variations of invasive pathways in lung cancersLung cancerE-cadherinTGF-bHAS-1, HAS-2 and HAS-3Hyal-1 and Hyal-3ImmunohistochemistryPrognosis and morphometryAdenocarcinomaAdenocarcinoma of LungAdultAgedAged, 80 and overCadherinsCarcinoma, Squamous CellCell Adhesion MoleculesExtracellular MatrixFemaleGlucuronosyltransferaseHumansHyaluronan SynthasesLung NeoplasmsMaleMiddle AgedNeoplasm InvasivenessNeoplasm StagingAmong the most common features of highly invasive tumors, such as lung adenocarcinomas (AD) and squamous cell carcinomas (SqCC), is the massive degradation of the extracellular matrix. The remarkable qualitative and quantitative modifications of hyaluronidases (HAases), hyaluronan synthases (HAS), E-cadherin adhesion molecules, and the transforming growth factor β (TGF-β) may favor invasion, cellular motility, and proliferation. We examined HAase proteins (Hyal), HAS, E-cadherin, and TGF-β profiles in lung AD subtypes and SqCC obtained from smokers and non-smokers. Fifty-six patients, median age 64 years, who underwent lobectomy for AD (N = 31) and SqCC (N = 25) were included in the study. HAS-1, -2 and -3, and Hyal-1 and -3 were significantly more expressed by tumor cells than normal and stroma cells (P < 0.01). When stratified according to histologic types, HAS-3 and Hyal-1 immunoreactivity was significantly increased in tumor cells of AD (P = 0.01) and stroma of SqCC (P = 0.002), respectively. Tobacco history in patients with AD was significantly associated with increased HAS-3 immunoreactivity in tumor cells (P < 0.01). Stroma cells of SqCC from non-smokers presented a significant association with HAS-3 (P < 0.01). Hyal, HAS, E-cadherin, and TGF-β modulate a different tumor-induced invasive pathway in lung AD subgroups and SqCC. HAases in resected AD and SqCC were strongly related to the prognosis. Therefore, our findings suggest that strategies aimed at preventing high HAS-3 and Hyal-1 synthesis, or local responses to low TGF-β and E-cadherin, may have a greater impact in lung cancer prognosis.Associacao Brasileira de Divulgacao Cientifica2013-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/109719http://hdl.handle.net/10316/109719https://doi.org/10.1590/1414-431x20122263engde Sá, V. K.Carvalho, L.Gomes, A.Alarcão, A.Silva, M. R.Couceiro, P.Sousa, V.Soares, F. A.Capelozzi, V. L.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-24T09:49:38Zoai:estudogeral.uc.pt:10316/109719Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:25:52.358955Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Role of the extracellular matrix in variations of invasive pathways in lung cancers
title Role of the extracellular matrix in variations of invasive pathways in lung cancers
spellingShingle Role of the extracellular matrix in variations of invasive pathways in lung cancers
de Sá, V. K.
Lung cancer
E-cadherin
TGF-b
HAS-1, HAS-2 and HAS-3
Hyal-1 and Hyal-3
Immunohistochemistry
Prognosis and morphometry
Adenocarcinoma
Adenocarcinoma of Lung
Adult
Aged
Aged, 80 and over
Cadherins
Carcinoma, Squamous Cell
Cell Adhesion Molecules
Extracellular Matrix
Female
Glucuronosyltransferase
Humans
Hyaluronan Synthases
Lung Neoplasms
Male
Middle Aged
Neoplasm Invasiveness
Neoplasm Staging
title_short Role of the extracellular matrix in variations of invasive pathways in lung cancers
title_full Role of the extracellular matrix in variations of invasive pathways in lung cancers
title_fullStr Role of the extracellular matrix in variations of invasive pathways in lung cancers
title_full_unstemmed Role of the extracellular matrix in variations of invasive pathways in lung cancers
title_sort Role of the extracellular matrix in variations of invasive pathways in lung cancers
author de Sá, V. K.
author_facet de Sá, V. K.
Carvalho, L.
Gomes, A.
Alarcão, A.
Silva, M. R.
Couceiro, P.
Sousa, V.
Soares, F. A.
Capelozzi, V. L.
author_role author
author2 Carvalho, L.
Gomes, A.
Alarcão, A.
Silva, M. R.
Couceiro, P.
Sousa, V.
Soares, F. A.
Capelozzi, V. L.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv de Sá, V. K.
Carvalho, L.
Gomes, A.
Alarcão, A.
Silva, M. R.
Couceiro, P.
Sousa, V.
Soares, F. A.
Capelozzi, V. L.
dc.subject.por.fl_str_mv Lung cancer
E-cadherin
TGF-b
HAS-1, HAS-2 and HAS-3
Hyal-1 and Hyal-3
Immunohistochemistry
Prognosis and morphometry
Adenocarcinoma
Adenocarcinoma of Lung
Adult
Aged
Aged, 80 and over
Cadherins
Carcinoma, Squamous Cell
Cell Adhesion Molecules
Extracellular Matrix
Female
Glucuronosyltransferase
Humans
Hyaluronan Synthases
Lung Neoplasms
Male
Middle Aged
Neoplasm Invasiveness
Neoplasm Staging
topic Lung cancer
E-cadherin
TGF-b
HAS-1, HAS-2 and HAS-3
Hyal-1 and Hyal-3
Immunohistochemistry
Prognosis and morphometry
Adenocarcinoma
Adenocarcinoma of Lung
Adult
Aged
Aged, 80 and over
Cadherins
Carcinoma, Squamous Cell
Cell Adhesion Molecules
Extracellular Matrix
Female
Glucuronosyltransferase
Humans
Hyaluronan Synthases
Lung Neoplasms
Male
Middle Aged
Neoplasm Invasiveness
Neoplasm Staging
description Among the most common features of highly invasive tumors, such as lung adenocarcinomas (AD) and squamous cell carcinomas (SqCC), is the massive degradation of the extracellular matrix. The remarkable qualitative and quantitative modifications of hyaluronidases (HAases), hyaluronan synthases (HAS), E-cadherin adhesion molecules, and the transforming growth factor β (TGF-β) may favor invasion, cellular motility, and proliferation. We examined HAase proteins (Hyal), HAS, E-cadherin, and TGF-β profiles in lung AD subtypes and SqCC obtained from smokers and non-smokers. Fifty-six patients, median age 64 years, who underwent lobectomy for AD (N = 31) and SqCC (N = 25) were included in the study. HAS-1, -2 and -3, and Hyal-1 and -3 were significantly more expressed by tumor cells than normal and stroma cells (P < 0.01). When stratified according to histologic types, HAS-3 and Hyal-1 immunoreactivity was significantly increased in tumor cells of AD (P = 0.01) and stroma of SqCC (P = 0.002), respectively. Tobacco history in patients with AD was significantly associated with increased HAS-3 immunoreactivity in tumor cells (P < 0.01). Stroma cells of SqCC from non-smokers presented a significant association with HAS-3 (P < 0.01). Hyal, HAS, E-cadherin, and TGF-β modulate a different tumor-induced invasive pathway in lung AD subgroups and SqCC. HAases in resected AD and SqCC were strongly related to the prognosis. Therefore, our findings suggest that strategies aimed at preventing high HAS-3 and Hyal-1 synthesis, or local responses to low TGF-β and E-cadherin, may have a greater impact in lung cancer prognosis.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/109719
http://hdl.handle.net/10316/109719
https://doi.org/10.1590/1414-431x20122263
url http://hdl.handle.net/10316/109719
https://doi.org/10.1590/1414-431x20122263
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Associacao Brasileira de Divulgacao Cientifica
publisher.none.fl_str_mv Associacao Brasileira de Divulgacao Cientifica
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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