Evaluation of wheat events transformed with the p5cs gene under conditions of water stress
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.19084/rca.17268 |
Resumo: | The enzyme ∆1-pyrroline-5-carboxylate synthetase (P5CS), encoded by the p5cs gene, is the rate-limiting step for proline biosynthesis, which acts as an osmoprotectant. The launch of transgenic commercial varieties depends on physiological and phenotypic characterization of events. The objective of this study was to evaluate five p5cs gene events (591, 14, 326, 164, 312), through physiological analyses and production rates during droughts. Plants of events and irrigated (IC) and stressed controls (SC) in flowering stage were submitted to 16 days of complete water restriction. The following elements were evaluated: relative water content (RWC); malondialdehyde concentration (MDA%); membrane stability index (MSI%); proline content; and production rates. As a result, the effect of water stress was only perceived after 12 days of water restriction with a decrease in the RWC values. Transgenic plants showed higher proline levels on the 16th day, compared to the SC (33 to 62%). The levels of MDA were lower on the 12th and 16th days for all the plants evaluated, but the MSI were similar to the IC for the events 591, 326, and 164, demonstrating the osmoprotectant effect. However, higher levels of proline did not result in higher production rates in the transgenic plants. |
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Evaluation of wheat events transformed with the p5cs gene under conditions of water stressAvaliação de eventos de trigo transformados com o gene p5cs e submetidos à secaGeralThe enzyme ∆1-pyrroline-5-carboxylate synthetase (P5CS), encoded by the p5cs gene, is the rate-limiting step for proline biosynthesis, which acts as an osmoprotectant. The launch of transgenic commercial varieties depends on physiological and phenotypic characterization of events. The objective of this study was to evaluate five p5cs gene events (591, 14, 326, 164, 312), through physiological analyses and production rates during droughts. Plants of events and irrigated (IC) and stressed controls (SC) in flowering stage were submitted to 16 days of complete water restriction. The following elements were evaluated: relative water content (RWC); malondialdehyde concentration (MDA%); membrane stability index (MSI%); proline content; and production rates. As a result, the effect of water stress was only perceived after 12 days of water restriction with a decrease in the RWC values. Transgenic plants showed higher proline levels on the 16th day, compared to the SC (33 to 62%). The levels of MDA were lower on the 12th and 16th days for all the plants evaluated, but the MSI were similar to the IC for the events 591, 326, and 164, demonstrating the osmoprotectant effect. However, higher levels of proline did not result in higher production rates in the transgenic plants.A enzima ∆1-pirrolina-5-carboxilato sintetase (P5CS), codificada pelo gene p5cs, é o passo limitante da biossíntese de prolina, que age como um osmoprotetor. O lançamento de variedades comerciais transgênicas depende da caracterização fisiológica e fenotípica de eventos. O objetivo deste estudo foi avaliar cinco eventos (591, 14, 326, 164, 312) contendo o gene p5cs, através de análises fisiológicas e taxas de produção sob estresse hídrico. Plantas de eventos e controles irrigados e estressados (CI e CS) em estádio de florescimento foram submetidos a 16 dias de restrição hídrica. Foram avaliados: teor relativo de água (RWC); concentração de malondialdeído (MDA%); índice de estabilidade da membrana (MSI%); conteúdo de prolina; e taxas de produção. Como resultado, o efeito do estresse hídrico só foi percebido após 12 dias de restrição hídrica com uma diminuição nos valores de RWC. Plantas transgênicas apresentaram maiores níveis de prolina no 16º dia, comparado ao SC (33 a 62%). Os teores de MDA foram menores no 12º e 16º dias para todas as plantas avaliadas, mas os MSI foram semelhantes ao IC para os eventos 591, 326 e 164, demonstrando o efeito osmoprotetor. Porém, níveis altos de prolina não resultaram em maiores taxas de produção de grãos.Sociedade de Ciências Agrárias de Portugal2019-05-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://doi.org/10.19084/rca.17268eng2183-041X0871-018XLima, Lettice Aparecida Dias Canette deSchuster, IvanCosta, António Carlos Torres daVendruscolo, Eliane C.G.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-06T09:25:14Zoai:ojs.revistas.rcaap.pt:article/17268Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:31:18.416726Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluation of wheat events transformed with the p5cs gene under conditions of water stress Avaliação de eventos de trigo transformados com o gene p5cs e submetidos à seca |
title |
Evaluation of wheat events transformed with the p5cs gene under conditions of water stress |
spellingShingle |
Evaluation of wheat events transformed with the p5cs gene under conditions of water stress Lima, Lettice Aparecida Dias Canette de Geral |
title_short |
Evaluation of wheat events transformed with the p5cs gene under conditions of water stress |
title_full |
Evaluation of wheat events transformed with the p5cs gene under conditions of water stress |
title_fullStr |
Evaluation of wheat events transformed with the p5cs gene under conditions of water stress |
title_full_unstemmed |
Evaluation of wheat events transformed with the p5cs gene under conditions of water stress |
title_sort |
Evaluation of wheat events transformed with the p5cs gene under conditions of water stress |
author |
Lima, Lettice Aparecida Dias Canette de |
author_facet |
Lima, Lettice Aparecida Dias Canette de Schuster, Ivan Costa, António Carlos Torres da Vendruscolo, Eliane C.G. |
author_role |
author |
author2 |
Schuster, Ivan Costa, António Carlos Torres da Vendruscolo, Eliane C.G. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Lima, Lettice Aparecida Dias Canette de Schuster, Ivan Costa, António Carlos Torres da Vendruscolo, Eliane C.G. |
dc.subject.por.fl_str_mv |
Geral |
topic |
Geral |
description |
The enzyme ∆1-pyrroline-5-carboxylate synthetase (P5CS), encoded by the p5cs gene, is the rate-limiting step for proline biosynthesis, which acts as an osmoprotectant. The launch of transgenic commercial varieties depends on physiological and phenotypic characterization of events. The objective of this study was to evaluate five p5cs gene events (591, 14, 326, 164, 312), through physiological analyses and production rates during droughts. Plants of events and irrigated (IC) and stressed controls (SC) in flowering stage were submitted to 16 days of complete water restriction. The following elements were evaluated: relative water content (RWC); malondialdehyde concentration (MDA%); membrane stability index (MSI%); proline content; and production rates. As a result, the effect of water stress was only perceived after 12 days of water restriction with a decrease in the RWC values. Transgenic plants showed higher proline levels on the 16th day, compared to the SC (33 to 62%). The levels of MDA were lower on the 12th and 16th days for all the plants evaluated, but the MSI were similar to the IC for the events 591, 326, and 164, demonstrating the osmoprotectant effect. However, higher levels of proline did not result in higher production rates in the transgenic plants. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-05-10T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.19084/rca.17268 |
url |
https://doi.org/10.19084/rca.17268 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2183-041X 0871-018X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Sociedade de Ciências Agrárias de Portugal |
publisher.none.fl_str_mv |
Sociedade de Ciências Agrárias de Portugal |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130178480242688 |