Extraction of potato cyst nematodes from soil samples: Cobb’s decanting and sieving method vs. Fenwick’s method
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.19084/rca.17061 |
Resumo: | Potato cyst nematodes are a threat to several agricultural crops around the world with some species considered quarantine pests and subjected to strict regulatory measures in many countries. Usually, cysts nematodes co-exist in the soil with other species of plant-parasitic nematodes, so, a time and cost-efficient extraction technique becomes of primary importance. The ideal extraction method should be able to obtain cysts as well as detecting the presence of other motile plant-parasitic nematodes with a potential impact on potato farming (such as Meloidogyne sp. and Pratylenchus sp.). In recent years, studies have been carried out to test the efficiency of various methods of nematode extraction but few results have been published. Therefore, to test if a method that extracts simultaneously cysts and motile nematodes can be used instead of the reference method that extracts cysts only, the efficiency of Cobb’s decanting and sieving technique was compared to Fenwick’s technique. As a result, in the 74 samples evaluated, a greater number of cysts were extracted from 24 samples using Fenwick’s method and from 11 samples employing Cobb’s decanting and sieving technique. The statistics results showed a significance level of 0,05 using Fenwick’s can allowing to conclude that this method is much more efficient than Cobb’s decanting and sieving technique, and confirming it should not be replaced by alternative methods for cysts extraction. |
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Extraction of potato cyst nematodes from soil samples: Cobb’s decanting and sieving method vs. Fenwick’s methodExtração de nemátodes de quisto de amostras de solo: método de decantação e crivagem de Cobb vs. método de FenwickGeralPotato cyst nematodes are a threat to several agricultural crops around the world with some species considered quarantine pests and subjected to strict regulatory measures in many countries. Usually, cysts nematodes co-exist in the soil with other species of plant-parasitic nematodes, so, a time and cost-efficient extraction technique becomes of primary importance. The ideal extraction method should be able to obtain cysts as well as detecting the presence of other motile plant-parasitic nematodes with a potential impact on potato farming (such as Meloidogyne sp. and Pratylenchus sp.). In recent years, studies have been carried out to test the efficiency of various methods of nematode extraction but few results have been published. Therefore, to test if a method that extracts simultaneously cysts and motile nematodes can be used instead of the reference method that extracts cysts only, the efficiency of Cobb’s decanting and sieving technique was compared to Fenwick’s technique. As a result, in the 74 samples evaluated, a greater number of cysts were extracted from 24 samples using Fenwick’s method and from 11 samples employing Cobb’s decanting and sieving technique. The statistics results showed a significance level of 0,05 using Fenwick’s can allowing to conclude that this method is much more efficient than Cobb’s decanting and sieving technique, and confirming it should not be replaced by alternative methods for cysts extraction.Os nemátodes de quisto constituem uma ameaça para diversas culturas agrícolas em todo o mundo. Algumas espécies são consideradas organismos nocivos de quarentena e encontram-se sujeitas a rigorosas medidas regulamentares em muitos países. Como os nemátodes de quisto coexistem no solo com outras espécies de nemátodes fitoparasitas, é necessário um método de extração que otimize custos e tempo. Idealmente, o método de extração deveria permitir detetar, para além dos quistos, a presença dos estádios juvenis de outros nemátodes fitoparasitas com potencial impacto agrícola (como Meloidogyne sp. e Pratylenchus sp.). Nos últimos anos, foram realizados alguns estudos para testar a eficiência de vários métodos de extração de nemátodos, mas poucos resultados foram publicados. Nesse sentido, foi comparada a eficiência do método de decantação e crivagem de Cobb com o método de Fenwick, visando testar se um método que extrai simultaneamente quistos e formas móveis pode ser usado em vez do método de referência para a extração de quistos. Nas 74 amostras avaliadas foi extraído maior número de quistos em 24 amostras pelo método de Fenwick e em 11 amostras pelo método de decantação e crivagem de Cobb. Estes resultados permitem concluir, para um nível de significância de 0,05, que o método de Fenwick foi mais eficiente que o de decantação e crivagem de Cobb na extração de quistos, não devendo ser substituído pelo outro método para este efeito.Sociedade de Ciências Agrárias de Portugal2019-03-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://doi.org/10.19084/rca.17061eng2183-041X0871-018XCamacho, Maria J.Mota, MarianaLima, ArlindoRusinque, LeidyInácio, Maria L.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-06T09:25:09Zoai:ojs.revistas.rcaap.pt:article/17061Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:31:13.825389Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Extraction of potato cyst nematodes from soil samples: Cobb’s decanting and sieving method vs. Fenwick’s method Extração de nemátodes de quisto de amostras de solo: método de decantação e crivagem de Cobb vs. método de Fenwick |
title |
Extraction of potato cyst nematodes from soil samples: Cobb’s decanting and sieving method vs. Fenwick’s method |
spellingShingle |
Extraction of potato cyst nematodes from soil samples: Cobb’s decanting and sieving method vs. Fenwick’s method Camacho, Maria J. Geral |
title_short |
Extraction of potato cyst nematodes from soil samples: Cobb’s decanting and sieving method vs. Fenwick’s method |
title_full |
Extraction of potato cyst nematodes from soil samples: Cobb’s decanting and sieving method vs. Fenwick’s method |
title_fullStr |
Extraction of potato cyst nematodes from soil samples: Cobb’s decanting and sieving method vs. Fenwick’s method |
title_full_unstemmed |
Extraction of potato cyst nematodes from soil samples: Cobb’s decanting and sieving method vs. Fenwick’s method |
title_sort |
Extraction of potato cyst nematodes from soil samples: Cobb’s decanting and sieving method vs. Fenwick’s method |
author |
Camacho, Maria J. |
author_facet |
Camacho, Maria J. Mota, Mariana Lima, Arlindo Rusinque, Leidy Inácio, Maria L. |
author_role |
author |
author2 |
Mota, Mariana Lima, Arlindo Rusinque, Leidy Inácio, Maria L. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Camacho, Maria J. Mota, Mariana Lima, Arlindo Rusinque, Leidy Inácio, Maria L. |
dc.subject.por.fl_str_mv |
Geral |
topic |
Geral |
description |
Potato cyst nematodes are a threat to several agricultural crops around the world with some species considered quarantine pests and subjected to strict regulatory measures in many countries. Usually, cysts nematodes co-exist in the soil with other species of plant-parasitic nematodes, so, a time and cost-efficient extraction technique becomes of primary importance. The ideal extraction method should be able to obtain cysts as well as detecting the presence of other motile plant-parasitic nematodes with a potential impact on potato farming (such as Meloidogyne sp. and Pratylenchus sp.). In recent years, studies have been carried out to test the efficiency of various methods of nematode extraction but few results have been published. Therefore, to test if a method that extracts simultaneously cysts and motile nematodes can be used instead of the reference method that extracts cysts only, the efficiency of Cobb’s decanting and sieving technique was compared to Fenwick’s technique. As a result, in the 74 samples evaluated, a greater number of cysts were extracted from 24 samples using Fenwick’s method and from 11 samples employing Cobb’s decanting and sieving technique. The statistics results showed a significance level of 0,05 using Fenwick’s can allowing to conclude that this method is much more efficient than Cobb’s decanting and sieving technique, and confirming it should not be replaced by alternative methods for cysts extraction. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-03-10T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.19084/rca.17061 |
url |
https://doi.org/10.19084/rca.17061 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2183-041X 0871-018X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Sociedade de Ciências Agrárias de Portugal |
publisher.none.fl_str_mv |
Sociedade de Ciências Agrárias de Portugal |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130177799716864 |