Vapor-phase of essential oils as a promising solution to prevent Candida vaginal biofilms caused by antifungal resistant strains

Detalhes bibliográficos
Autor(a) principal: Fernandes, Liliana Sousa
Data de Publicação: 2022
Outros Autores: Gonçalves, Bruna Fernandes, Costa, Raquel, Fernandes, Ângela, Gomes, Ana, Nogueira-Silva, Cristina, Silva, Sónia Carina, Rodrigues, Maria Elisa Costa, Henriques, Mariana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/79412
Resumo: Background: Vulvovaginal candidiasis (VVC) is a disease with high incidence, a huge impact on the quality of life and health of women, and which represents a great challenge to treat. The growing need to apply antifungal intensive therapies have contributed to an emergence of drug-resistant Candida strains. Thus, effective therapeutic options, to meet the antifungal-resistance challenge and to control high resilient biofilms, are urgently needed. This study aimed to investigate the antifungal activity of essentials oils (EOs) on drug-resistant Candida vaginal isolates. Method: Therefore, the antimicrobial effect of tea tree, niaouli, white thyme, and cajeput EOs on the planktonic growth of Candida isolates was initially evaluated by an agar disc diffusion method. Then, the vapor-phase effect of tea tree EO (VP-TTEO) on biofilm formation and on pre-formed biofilms was evaluated by crystal violet staining, XTT reduction assay, colony forming units’ enumeration, and scanning electron microscopy. Results: The results revealed high antifungal activity of EOs against drug-resistant Candida isolates. Additionally, the VP-TTEO showed a significant inhibitory effect on the biofilm formation of all tested isolates and was able to provoke an expressive reduction in mature Candida albicans biofilms. Conclusions: Overall, this study suggests that the VP-EO may be a promising solution that is able to prevent biofilm-related VVC caused by antifungal-resistant strains.
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spelling Vapor-phase of essential oils as a promising solution to prevent Candida vaginal biofilms caused by antifungal resistant strainsvulvovaginal candidiasisbiofilmantifungal resistancealternative therapiesessential oilvapor-phasephytotherapeutic applicationScience & TechnologyBackground: Vulvovaginal candidiasis (VVC) is a disease with high incidence, a huge impact on the quality of life and health of women, and which represents a great challenge to treat. The growing need to apply antifungal intensive therapies have contributed to an emergence of drug-resistant Candida strains. Thus, effective therapeutic options, to meet the antifungal-resistance challenge and to control high resilient biofilms, are urgently needed. This study aimed to investigate the antifungal activity of essentials oils (EOs) on drug-resistant Candida vaginal isolates. Method: Therefore, the antimicrobial effect of tea tree, niaouli, white thyme, and cajeput EOs on the planktonic growth of Candida isolates was initially evaluated by an agar disc diffusion method. Then, the vapor-phase effect of tea tree EO (VP-TTEO) on biofilm formation and on pre-formed biofilms was evaluated by crystal violet staining, XTT reduction assay, colony forming units’ enumeration, and scanning electron microscopy. Results: The results revealed high antifungal activity of EOs against drug-resistant Candida isolates. Additionally, the VP-TTEO showed a significant inhibitory effect on the biofilm formation of all tested isolates and was able to provoke an expressive reduction in mature Candida albicans biofilms. Conclusions: Overall, this study suggests that the VP-EO may be a promising solution that is able to prevent biofilm-related VVC caused by antifungal-resistant strains.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and grant ref 2020.05720.BD for Liliana Fernandes. Also, this study was supported by LABBELS—Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020 and Maria Elisa Rodrigues thanks FCT for funding through program DL 57/2016—Norma transitória.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoFernandes, Liliana SousaGonçalves, Bruna FernandesCosta, RaquelFernandes, ÂngelaGomes, AnaNogueira-Silva, CristinaSilva, Sónia CarinaRodrigues, Maria Elisa CostaHenriques, Mariana2022-08-292022-08-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/79412engFernandes, L.; Gonçalves, B.; Costa, R.; Fernandes, Â.; Gomes, A.; Nogueira-Silva, C.; Silva, S.; Rodrigues, M.E.; Henriques, M. Vapor-Phase of Essential Oils as a Promising Solution to Prevent Candida Vaginal Biofilms Caused by Antifungal Resistant Strains. Healthcare 2022, 10, 1649. https://doi.org/10.3390/healthcare100916492227-903210.3390/healthcare10091649https://www.mdpi.com/2227-9032/10/9/1649info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:12:01Zoai:repositorium.sdum.uminho.pt:1822/79412Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:03:54.586791Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Vapor-phase of essential oils as a promising solution to prevent Candida vaginal biofilms caused by antifungal resistant strains
title Vapor-phase of essential oils as a promising solution to prevent Candida vaginal biofilms caused by antifungal resistant strains
spellingShingle Vapor-phase of essential oils as a promising solution to prevent Candida vaginal biofilms caused by antifungal resistant strains
Fernandes, Liliana Sousa
vulvovaginal candidiasis
biofilm
antifungal resistance
alternative therapies
essential oil
vapor-phase
phytotherapeutic application
Science & Technology
title_short Vapor-phase of essential oils as a promising solution to prevent Candida vaginal biofilms caused by antifungal resistant strains
title_full Vapor-phase of essential oils as a promising solution to prevent Candida vaginal biofilms caused by antifungal resistant strains
title_fullStr Vapor-phase of essential oils as a promising solution to prevent Candida vaginal biofilms caused by antifungal resistant strains
title_full_unstemmed Vapor-phase of essential oils as a promising solution to prevent Candida vaginal biofilms caused by antifungal resistant strains
title_sort Vapor-phase of essential oils as a promising solution to prevent Candida vaginal biofilms caused by antifungal resistant strains
author Fernandes, Liliana Sousa
author_facet Fernandes, Liliana Sousa
Gonçalves, Bruna Fernandes
Costa, Raquel
Fernandes, Ângela
Gomes, Ana
Nogueira-Silva, Cristina
Silva, Sónia Carina
Rodrigues, Maria Elisa Costa
Henriques, Mariana
author_role author
author2 Gonçalves, Bruna Fernandes
Costa, Raquel
Fernandes, Ângela
Gomes, Ana
Nogueira-Silva, Cristina
Silva, Sónia Carina
Rodrigues, Maria Elisa Costa
Henriques, Mariana
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Fernandes, Liliana Sousa
Gonçalves, Bruna Fernandes
Costa, Raquel
Fernandes, Ângela
Gomes, Ana
Nogueira-Silva, Cristina
Silva, Sónia Carina
Rodrigues, Maria Elisa Costa
Henriques, Mariana
dc.subject.por.fl_str_mv vulvovaginal candidiasis
biofilm
antifungal resistance
alternative therapies
essential oil
vapor-phase
phytotherapeutic application
Science & Technology
topic vulvovaginal candidiasis
biofilm
antifungal resistance
alternative therapies
essential oil
vapor-phase
phytotherapeutic application
Science & Technology
description Background: Vulvovaginal candidiasis (VVC) is a disease with high incidence, a huge impact on the quality of life and health of women, and which represents a great challenge to treat. The growing need to apply antifungal intensive therapies have contributed to an emergence of drug-resistant Candida strains. Thus, effective therapeutic options, to meet the antifungal-resistance challenge and to control high resilient biofilms, are urgently needed. This study aimed to investigate the antifungal activity of essentials oils (EOs) on drug-resistant Candida vaginal isolates. Method: Therefore, the antimicrobial effect of tea tree, niaouli, white thyme, and cajeput EOs on the planktonic growth of Candida isolates was initially evaluated by an agar disc diffusion method. Then, the vapor-phase effect of tea tree EO (VP-TTEO) on biofilm formation and on pre-formed biofilms was evaluated by crystal violet staining, XTT reduction assay, colony forming units’ enumeration, and scanning electron microscopy. Results: The results revealed high antifungal activity of EOs against drug-resistant Candida isolates. Additionally, the VP-TTEO showed a significant inhibitory effect on the biofilm formation of all tested isolates and was able to provoke an expressive reduction in mature Candida albicans biofilms. Conclusions: Overall, this study suggests that the VP-EO may be a promising solution that is able to prevent biofilm-related VVC caused by antifungal-resistant strains.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-29
2022-08-29T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/79412
url https://hdl.handle.net/1822/79412
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fernandes, L.; Gonçalves, B.; Costa, R.; Fernandes, Â.; Gomes, A.; Nogueira-Silva, C.; Silva, S.; Rodrigues, M.E.; Henriques, M. Vapor-Phase of Essential Oils as a Promising Solution to Prevent Candida Vaginal Biofilms Caused by Antifungal Resistant Strains. Healthcare 2022, 10, 1649. https://doi.org/10.3390/healthcare10091649
2227-9032
10.3390/healthcare10091649
https://www.mdpi.com/2227-9032/10/9/1649
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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