Establishment and elicitation of liquid adventitious root cultures of Inula crithmoides L. for increased caffeoylquinic acids production and hepatoprotective properties
Autor(a) principal: | |
---|---|
Data de Publicação: | 2024 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/20363 |
Resumo: | This study established a liquid adventitious root culture for Inula crithmoides L. focusing on producing extracts rich in hepatoprotective caffeoylquinic acids through elicitation. Adventitious roots were induced from in vitro-grown plant leaves cultured on MS medium supplemented with 1 mg/L IBA. Growth and secondary metabolites accumulation (phenolics, flavonoids, hydroxycinnamic acids) were monitored weekly to determine the optimal harvesting time. Then, the adventitious roots were elicited for 4 weeks with varying concentrations of yeast extract-YE (50, 100 and 200 mg/L) and extracted with ethanol. The extracts were evaluated for their hepatoprotective effects against ethanol-induced hepatotoxicity and analyzed through high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). Roots of greenhouse-cultivated plants were used to compare the results obtained from in vitro -grown adventitious roots. IBA supplementation induced 100% adventitious root formation, with maximum biomass and phenolic accumulation after 4 weeks. Hydroxycinnamic acid levels increased significantly with YE treatment at all the concentrations, particularly in case of chlorogenic acid and di-O-caffeoylquinic acid isomers. The root extracts displayed significant in vitro hepatoprotective effects, particularly at 50 mg/L YE elicitation. Overall, the elicitation of I. crithmoides liquid root cultures with yeast extract offers a promising, efficient, and cost-effective approach for optimizing the production of valuable caffeoylquinic acids having potential pharmaceutical use. |
id |
RCAP_c1852e711ccb3c1f7db91900f79c433e |
---|---|
oai_identifier_str |
oai:sapientia.ualg.pt:10400.1/20363 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Establishment and elicitation of liquid adventitious root cultures of Inula crithmoides L. for increased caffeoylquinic acids production and hepatoprotective propertiesGolden samphireAsteraceaePlant tissue cultureYeast extractThis study established a liquid adventitious root culture for Inula crithmoides L. focusing on producing extracts rich in hepatoprotective caffeoylquinic acids through elicitation. Adventitious roots were induced from in vitro-grown plant leaves cultured on MS medium supplemented with 1 mg/L IBA. Growth and secondary metabolites accumulation (phenolics, flavonoids, hydroxycinnamic acids) were monitored weekly to determine the optimal harvesting time. Then, the adventitious roots were elicited for 4 weeks with varying concentrations of yeast extract-YE (50, 100 and 200 mg/L) and extracted with ethanol. The extracts were evaluated for their hepatoprotective effects against ethanol-induced hepatotoxicity and analyzed through high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). Roots of greenhouse-cultivated plants were used to compare the results obtained from in vitro -grown adventitious roots. IBA supplementation induced 100% adventitious root formation, with maximum biomass and phenolic accumulation after 4 weeks. Hydroxycinnamic acid levels increased significantly with YE treatment at all the concentrations, particularly in case of chlorogenic acid and di-O-caffeoylquinic acid isomers. The root extracts displayed significant in vitro hepatoprotective effects, particularly at 50 mg/L YE elicitation. Overall, the elicitation of I. crithmoides liquid root cultures with yeast extract offers a promising, efficient, and cost-effective approach for optimizing the production of valuable caffeoylquinic acids having potential pharmaceutical use.SpringerSapientiaCustódio, LuísaCziáky, ZoltánCastañeda-Loaiza, VianaRodrigues, Maria Joao2024-02-03T12:47:58Z20242024-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/20363eng0167-685710.1007/s11240-024-02690-zinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-07T02:01:21Zoai:sapientia.ualg.pt:10400.1/20363Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:36:39.278152Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Establishment and elicitation of liquid adventitious root cultures of Inula crithmoides L. for increased caffeoylquinic acids production and hepatoprotective properties |
title |
Establishment and elicitation of liquid adventitious root cultures of Inula crithmoides L. for increased caffeoylquinic acids production and hepatoprotective properties |
spellingShingle |
Establishment and elicitation of liquid adventitious root cultures of Inula crithmoides L. for increased caffeoylquinic acids production and hepatoprotective properties Custódio, Luísa Golden samphire Asteraceae Plant tissue culture Yeast extract |
title_short |
Establishment and elicitation of liquid adventitious root cultures of Inula crithmoides L. for increased caffeoylquinic acids production and hepatoprotective properties |
title_full |
Establishment and elicitation of liquid adventitious root cultures of Inula crithmoides L. for increased caffeoylquinic acids production and hepatoprotective properties |
title_fullStr |
Establishment and elicitation of liquid adventitious root cultures of Inula crithmoides L. for increased caffeoylquinic acids production and hepatoprotective properties |
title_full_unstemmed |
Establishment and elicitation of liquid adventitious root cultures of Inula crithmoides L. for increased caffeoylquinic acids production and hepatoprotective properties |
title_sort |
Establishment and elicitation of liquid adventitious root cultures of Inula crithmoides L. for increased caffeoylquinic acids production and hepatoprotective properties |
author |
Custódio, Luísa |
author_facet |
Custódio, Luísa Cziáky, Zoltán Castañeda-Loaiza, Viana Rodrigues, Maria Joao |
author_role |
author |
author2 |
Cziáky, Zoltán Castañeda-Loaiza, Viana Rodrigues, Maria Joao |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Custódio, Luísa Cziáky, Zoltán Castañeda-Loaiza, Viana Rodrigues, Maria Joao |
dc.subject.por.fl_str_mv |
Golden samphire Asteraceae Plant tissue culture Yeast extract |
topic |
Golden samphire Asteraceae Plant tissue culture Yeast extract |
description |
This study established a liquid adventitious root culture for Inula crithmoides L. focusing on producing extracts rich in hepatoprotective caffeoylquinic acids through elicitation. Adventitious roots were induced from in vitro-grown plant leaves cultured on MS medium supplemented with 1 mg/L IBA. Growth and secondary metabolites accumulation (phenolics, flavonoids, hydroxycinnamic acids) were monitored weekly to determine the optimal harvesting time. Then, the adventitious roots were elicited for 4 weeks with varying concentrations of yeast extract-YE (50, 100 and 200 mg/L) and extracted with ethanol. The extracts were evaluated for their hepatoprotective effects against ethanol-induced hepatotoxicity and analyzed through high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). Roots of greenhouse-cultivated plants were used to compare the results obtained from in vitro -grown adventitious roots. IBA supplementation induced 100% adventitious root formation, with maximum biomass and phenolic accumulation after 4 weeks. Hydroxycinnamic acid levels increased significantly with YE treatment at all the concentrations, particularly in case of chlorogenic acid and di-O-caffeoylquinic acid isomers. The root extracts displayed significant in vitro hepatoprotective effects, particularly at 50 mg/L YE elicitation. Overall, the elicitation of I. crithmoides liquid root cultures with yeast extract offers a promising, efficient, and cost-effective approach for optimizing the production of valuable caffeoylquinic acids having potential pharmaceutical use. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-02-03T12:47:58Z 2024 2024-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/20363 |
url |
http://hdl.handle.net/10400.1/20363 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0167-6857 10.1007/s11240-024-02690-z |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799137417671737344 |