Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents

Detalhes bibliográficos
Autor(a) principal: Neto, Salomé
Data de Publicação: 2023
Outros Autores: Mendes, João P., Santos, Susana B.Dos, Solbrand, Anita, Carrondo, Manuel J.T., Peixoto, Cristina, Silva, Ricardo J.S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158522
Resumo: Funding Information: The authors acknowledge the funding of Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), projects PTDC/EQU-EQU/0142/2020 and EXPL/EQU-EQU/1567/2021; SN is the recipient of an FCT fellowship from the project PTDC/EQU-EQU/0142/2020. Publisher Copyright: Copyright © 2023 Neto, Mendes, Santos, Solbrand, Carrondo, Peixoto and Silva.
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spelling Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbentsAAV5affinity chromatographycellulose-based nanofibersgene therapynon-woven materialsBiotechnologyBioengineeringHistologyBiomedical EngineeringFunding Information: The authors acknowledge the funding of Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), projects PTDC/EQU-EQU/0142/2020 and EXPL/EQU-EQU/1567/2021; SN is the recipient of an FCT fellowship from the project PTDC/EQU-EQU/0142/2020. Publisher Copyright: Copyright © 2023 Neto, Mendes, Santos, Solbrand, Carrondo, Peixoto and Silva.Adeno-associated viruses (AAVs) are one of the most promising tools for gene therapy applications. These vectors are purified using affinity and ion exchange chromatography, typically using packed beds of resin adsorbents. This leads to diffusion and pressure drop limitations that affect process productivity. Due to their high surface area and porosity, electrospun nanofiber adsorbents offer mass transfer and flow rate advantages over conventional chromatographic media. The present work investigated the use of affinity cellulose-based nanofiber adsorbents for adeno-associated virus serotype 5 (AAV5) capture, evaluating dynamic binding capacity, pressure drop, and AAV5 recovery at residence times (RT) less than 5 s. The dynamic binding capacity was found to be residence time-dependent, but nevertheless higher than 1.0 × 1014 TP mL−1 (RT = 1.6 s), with a pressure drop variation of 0.14 MPa obtained after loading more than 2,000 column volumes of clarified AAV5 feedstock. The single affinity chromatography purification step using these new affinity adsorbents resulted in 80% virus recovery, with the removal of impurities comparable to that of bead-based affinity adsorbents. The high binding capacity, virus recovery and reduced pressure drop observed at residence times in the sub-minute range can potentially eliminate the need for prior concentration steps, thereby reducing the overall number of unit operations, process time and costs.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNNeto, SaloméMendes, João P.Santos, Susana B.DosSolbrand, AnitaCarrondo, Manuel J.T.Peixoto, CristinaSilva, Ricardo J.S.2023-09-30T22:18:45Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158522eng2296-4185PURE: 72364677https://doi.org/10.3389/fbioe.2023.1183974info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:40:59Zoai:run.unl.pt:10362/158522Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:11.143582Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents
title Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents
spellingShingle Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents
Neto, Salomé
AAV5
affinity chromatography
cellulose-based nanofibers
gene therapy
non-woven materials
Biotechnology
Bioengineering
Histology
Biomedical Engineering
title_short Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents
title_full Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents
title_fullStr Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents
title_full_unstemmed Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents
title_sort Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents
author Neto, Salomé
author_facet Neto, Salomé
Mendes, João P.
Santos, Susana B.Dos
Solbrand, Anita
Carrondo, Manuel J.T.
Peixoto, Cristina
Silva, Ricardo J.S.
author_role author
author2 Mendes, João P.
Santos, Susana B.Dos
Solbrand, Anita
Carrondo, Manuel J.T.
Peixoto, Cristina
Silva, Ricardo J.S.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Neto, Salomé
Mendes, João P.
Santos, Susana B.Dos
Solbrand, Anita
Carrondo, Manuel J.T.
Peixoto, Cristina
Silva, Ricardo J.S.
dc.subject.por.fl_str_mv AAV5
affinity chromatography
cellulose-based nanofibers
gene therapy
non-woven materials
Biotechnology
Bioengineering
Histology
Biomedical Engineering
topic AAV5
affinity chromatography
cellulose-based nanofibers
gene therapy
non-woven materials
Biotechnology
Bioengineering
Histology
Biomedical Engineering
description Funding Information: The authors acknowledge the funding of Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), projects PTDC/EQU-EQU/0142/2020 and EXPL/EQU-EQU/1567/2021; SN is the recipient of an FCT fellowship from the project PTDC/EQU-EQU/0142/2020. Publisher Copyright: Copyright © 2023 Neto, Mendes, Santos, Solbrand, Carrondo, Peixoto and Silva.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-30T22:18:45Z
2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158522
url http://hdl.handle.net/10362/158522
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2296-4185
PURE: 72364677
https://doi.org/10.3389/fbioe.2023.1183974
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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