Gene silencing by siRNA nanoparticles synthesized via sonochemical method

Detalhes bibliográficos
Autor(a) principal: Shimanovich, Ulyana
Data de Publicação: 2014
Outros Autores: Munder, A., Loureiro, Ana, Azóia, Nuno G., Gomes, Andreia, Paulo, Artur Cavaco, Gedanken, Aharon, Gruzman, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/31685
Resumo: The knowledge that small RNAs can affect gene expression has had a tremendous impact on basic and applied research, and gene silencing is currently one of the most promising new approaches for disease therapy. However, RNAs cannot easily penetrate cell membranes, therefore RNA delivery become one of the major challenges for gene silencing technology. In the current paper we discuss a general approach for converting siRNA molecules into a dense siRNA nanoparticles using environmentally friendly sonochemical method. The RNA nanoparticulation enhance its gene-silencing activity in vascular bovine endothelial as well as in cancer 293T/GFP-Puro cell lines without causing any toxic effect. We show that ultrasonic waves do not lead to RNA degradation or any changes in its chemical structure. Moreover, sonochemically produced siRNA nanoparticles have been shown to be resistant to a variety of environmental stresses including pH levels, enzymes and temperatures, hence solving problem of the short half-life of the RNA molecules. As the siRNA nanoparticles are biocompatibile and biodegradabile, and their RNA release properties may be controlled within limits, sonochemical formation of siRNA nanoparticles represent a new promising approach for generation of functional bionano materials.
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spelling Gene silencing by siRNA nanoparticles synthesized via sonochemical methodNanoparticlesBionano materialssiRNA nanoparticlesGene silencingThe knowledge that small RNAs can affect gene expression has had a tremendous impact on basic and applied research, and gene silencing is currently one of the most promising new approaches for disease therapy. However, RNAs cannot easily penetrate cell membranes, therefore RNA delivery become one of the major challenges for gene silencing technology. In the current paper we discuss a general approach for converting siRNA molecules into a dense siRNA nanoparticles using environmentally friendly sonochemical method. The RNA nanoparticulation enhance its gene-silencing activity in vascular bovine endothelial as well as in cancer 293T/GFP-Puro cell lines without causing any toxic effect. We show that ultrasonic waves do not lead to RNA degradation or any changes in its chemical structure. Moreover, sonochemically produced siRNA nanoparticles have been shown to be resistant to a variety of environmental stresses including pH levels, enzymes and temperatures, hence solving problem of the short half-life of the RNA molecules. As the siRNA nanoparticles are biocompatibile and biodegradabile, and their RNA release properties may be controlled within limits, sonochemical formation of siRNA nanoparticles represent a new promising approach for generation of functional bionano materials.(undefined)OMICS Publishing GroupUniversidade do MinhoShimanovich, UlyanaMunder, A.Loureiro, AnaAzóia, Nuno G.Gomes, AndreiaPaulo, Artur CavacoGedanken, AharonGruzman, A.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31685eng2157-74392157-743910.4172/2157-7439.1000204http://omicsonline.org/nanomedicine-nanotechnology.phpinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:34:09Zoai:repositorium.sdum.uminho.pt:1822/31685Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:29:46.410481Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gene silencing by siRNA nanoparticles synthesized via sonochemical method
title Gene silencing by siRNA nanoparticles synthesized via sonochemical method
spellingShingle Gene silencing by siRNA nanoparticles synthesized via sonochemical method
Shimanovich, Ulyana
Nanoparticles
Bionano materials
siRNA nanoparticles
Gene silencing
title_short Gene silencing by siRNA nanoparticles synthesized via sonochemical method
title_full Gene silencing by siRNA nanoparticles synthesized via sonochemical method
title_fullStr Gene silencing by siRNA nanoparticles synthesized via sonochemical method
title_full_unstemmed Gene silencing by siRNA nanoparticles synthesized via sonochemical method
title_sort Gene silencing by siRNA nanoparticles synthesized via sonochemical method
author Shimanovich, Ulyana
author_facet Shimanovich, Ulyana
Munder, A.
Loureiro, Ana
Azóia, Nuno G.
Gomes, Andreia
Paulo, Artur Cavaco
Gedanken, Aharon
Gruzman, A.
author_role author
author2 Munder, A.
Loureiro, Ana
Azóia, Nuno G.
Gomes, Andreia
Paulo, Artur Cavaco
Gedanken, Aharon
Gruzman, A.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Shimanovich, Ulyana
Munder, A.
Loureiro, Ana
Azóia, Nuno G.
Gomes, Andreia
Paulo, Artur Cavaco
Gedanken, Aharon
Gruzman, A.
dc.subject.por.fl_str_mv Nanoparticles
Bionano materials
siRNA nanoparticles
Gene silencing
topic Nanoparticles
Bionano materials
siRNA nanoparticles
Gene silencing
description The knowledge that small RNAs can affect gene expression has had a tremendous impact on basic and applied research, and gene silencing is currently one of the most promising new approaches for disease therapy. However, RNAs cannot easily penetrate cell membranes, therefore RNA delivery become one of the major challenges for gene silencing technology. In the current paper we discuss a general approach for converting siRNA molecules into a dense siRNA nanoparticles using environmentally friendly sonochemical method. The RNA nanoparticulation enhance its gene-silencing activity in vascular bovine endothelial as well as in cancer 293T/GFP-Puro cell lines without causing any toxic effect. We show that ultrasonic waves do not lead to RNA degradation or any changes in its chemical structure. Moreover, sonochemically produced siRNA nanoparticles have been shown to be resistant to a variety of environmental stresses including pH levels, enzymes and temperatures, hence solving problem of the short half-life of the RNA molecules. As the siRNA nanoparticles are biocompatibile and biodegradabile, and their RNA release properties may be controlled within limits, sonochemical formation of siRNA nanoparticles represent a new promising approach for generation of functional bionano materials.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/31685
url http://hdl.handle.net/1822/31685
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2157-7439
2157-7439
10.4172/2157-7439.1000204
http://omicsonline.org/nanomedicine-nanotechnology.php
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv OMICS Publishing Group
publisher.none.fl_str_mv OMICS Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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