Genomic Analysis of a mcr-9.1-Harbouring IncHI2-ST1 Plasmid from Enterobacter ludwigii Isolated in Fish Farming

Detalhes bibliográficos
Autor(a) principal: Manageiro, Vera
Data de Publicação: 2022
Outros Autores: Salgueiro, Vanessa, Rosado, Tânia, Bandarra, Narcisa M., Ferreira, Eugénia, Smith, Terry, Dias, Elsa, Caniça, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8558
Resumo: This article belongs to the Special Issue Antibiotic Resistance and Virulence Profiles of Gram-Negative Bacteria.
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spelling Genomic Analysis of a mcr-9.1-Harbouring IncHI2-ST1 Plasmid from Enterobacter ludwigii Isolated in Fish FarmingOne HealthAquaculturemcr-9 GeneSeabreamEnterobacter ludwigiiEnterobacter sp.Resistência aos AntimicrobianosGenotoxicidade AmbientalThis article belongs to the Special Issue Antibiotic Resistance and Virulence Profiles of Gram-Negative Bacteria.This study analyzed the resistome, virulome and mobilome of an MCR-9-producing Enterobacter sp. identified in a muscle sample of seabream (Sparus aurata), collected in a land tank from multitrophic fish farming production. Average Nucleotide Identity analysis identified INSAq77 at the species level as an Enterobacter ludwigii INSAq77 strain that was resistant to chloramphenicol, florfenicol and fosfomycin and was susceptible to all other antibiotics tested. In silico antimicrobial resistance analyses revealed genes conferring in silico resistance to β-lactams (blaACT-88), chloramphenicol (catA4-type), fosfomycin (fosA2-type) and colistin (mcr-9.1), as well as several efflux pumps (e.g., oqxAB-type and mar operon). Further bioinformatics analysis revealed five plasmid replicon types, including the IncHI2/HI2A, which are linked to the worldwide dissemination of the mcr-9 gene in different antibiotic resistance reservoirs. The conserved nickel/copper operon rcnR-rcnA-pcoE-ISSgsp1-pcoS-IS903-mcr-9-wbuC was present, which may play a key role in copper tolerance under anaerobic growth and nickel homeostasis. These results highlight that antibiotic resistance in aquaculture are spreading through food, the environment and humans, which places this research in a One Health context. In fact, colistin is used as a last resort for the treatment of serious infections in clinical settings, thus mcr genes may represent a serious threat to human health.V.S. had her Ph.D. fellowship granted by FCT (Fundação para a Ciência e a Tecnologia) [SFRH/BD/133100/2017], co-financed by European Social Fund and the Operational Program for Human Capital (POCH), Portugal. The work was supported with funding from FCT/MCTES [UIDB/00211/2020] through national funds. This work was partially supported by funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 773830: One Health European Joint Programme (WORLDCOM project), and partially from FCT/MCTES [UIDB/00211/2020] through national funds.MDPIRepositório Científico do Instituto Nacional de SaúdeManageiro, VeraSalgueiro, VanessaRosado, TâniaBandarra, Narcisa M.Ferreira, EugéniaSmith, TerryDias, ElsaCaniça, Manuela2023-03-17T15:23:35Z2022-09-102022-09-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8558engAntibiotics (Basel). 2022 Sep 10;11(9):1232. doi: 10.3390/antibiotics110912322079-638210.3390/antibiotics11091232info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:38Zoai:repositorio.insa.pt:10400.18/8558Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:43:11.967228Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genomic Analysis of a mcr-9.1-Harbouring IncHI2-ST1 Plasmid from Enterobacter ludwigii Isolated in Fish Farming
title Genomic Analysis of a mcr-9.1-Harbouring IncHI2-ST1 Plasmid from Enterobacter ludwigii Isolated in Fish Farming
spellingShingle Genomic Analysis of a mcr-9.1-Harbouring IncHI2-ST1 Plasmid from Enterobacter ludwigii Isolated in Fish Farming
Manageiro, Vera
One Health
Aquaculture
mcr-9 Gene
Seabream
Enterobacter ludwigii
Enterobacter sp.
Resistência aos Antimicrobianos
Genotoxicidade Ambiental
title_short Genomic Analysis of a mcr-9.1-Harbouring IncHI2-ST1 Plasmid from Enterobacter ludwigii Isolated in Fish Farming
title_full Genomic Analysis of a mcr-9.1-Harbouring IncHI2-ST1 Plasmid from Enterobacter ludwigii Isolated in Fish Farming
title_fullStr Genomic Analysis of a mcr-9.1-Harbouring IncHI2-ST1 Plasmid from Enterobacter ludwigii Isolated in Fish Farming
title_full_unstemmed Genomic Analysis of a mcr-9.1-Harbouring IncHI2-ST1 Plasmid from Enterobacter ludwigii Isolated in Fish Farming
title_sort Genomic Analysis of a mcr-9.1-Harbouring IncHI2-ST1 Plasmid from Enterobacter ludwigii Isolated in Fish Farming
author Manageiro, Vera
author_facet Manageiro, Vera
Salgueiro, Vanessa
Rosado, Tânia
Bandarra, Narcisa M.
Ferreira, Eugénia
Smith, Terry
Dias, Elsa
Caniça, Manuela
author_role author
author2 Salgueiro, Vanessa
Rosado, Tânia
Bandarra, Narcisa M.
Ferreira, Eugénia
Smith, Terry
Dias, Elsa
Caniça, Manuela
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Manageiro, Vera
Salgueiro, Vanessa
Rosado, Tânia
Bandarra, Narcisa M.
Ferreira, Eugénia
Smith, Terry
Dias, Elsa
Caniça, Manuela
dc.subject.por.fl_str_mv One Health
Aquaculture
mcr-9 Gene
Seabream
Enterobacter ludwigii
Enterobacter sp.
Resistência aos Antimicrobianos
Genotoxicidade Ambiental
topic One Health
Aquaculture
mcr-9 Gene
Seabream
Enterobacter ludwigii
Enterobacter sp.
Resistência aos Antimicrobianos
Genotoxicidade Ambiental
description This article belongs to the Special Issue Antibiotic Resistance and Virulence Profiles of Gram-Negative Bacteria.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-10
2022-09-10T00:00:00Z
2023-03-17T15:23:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8558
url http://hdl.handle.net/10400.18/8558
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Antibiotics (Basel). 2022 Sep 10;11(9):1232. doi: 10.3390/antibiotics11091232
2079-6382
10.3390/antibiotics11091232
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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