Potential prebiotic activity of Tenebrio molitor insect flour using an optimized in vitro gut microbiota model
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/27650 |
Resumo: | The Tenebrio molitor insect flour (TMIF) is considered a nutritive food ingredient, but its impact at the gut microbiota level and its potential prebiotic activity still need be assessed. For such studies, an in vitro simulation model of gut microbiota was optimized. Pure cultures of Lactobacillus and Bifidobacterium strains in monocultures and co-cultures (pairs and consortium) were used in this model to evaluate the effect of TMIF on the viability and metabolic activity of those bacteria. The optimization of the in vitro model of the gut microbiota was successful, and growth of the most important groups of bacteria in the gut microbiota was observed. So this model can be used to study the effects of other ingredients at that level. It also enabled pinpointing the prebiotic effects of the studied TMIF suggesting possible symbiotic interactions. In addition, the direct effect of the TMIF on bacterial cells, when in nutritive stress was also evaluated. In terms of TMIF effects on probiotic bacteria viability and growth, no negative effects were observed, and even an enhancement of growth and an increase of production of short chain fatty acids (SCFA) and lactate, in most of the cases, was observed. Also, this study showed that TMIF helps in maintaining the viability of bacteria during incubation time when these are under nutritional stress conditions. A potential prebiotic effect of TMIF is then predictable with this study, opening a path to new research on this subject. |
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Potential prebiotic activity of Tenebrio molitor insect flour using an optimized in vitro gut microbiota modelThe Tenebrio molitor insect flour (TMIF) is considered a nutritive food ingredient, but its impact at the gut microbiota level and its potential prebiotic activity still need be assessed. For such studies, an in vitro simulation model of gut microbiota was optimized. Pure cultures of Lactobacillus and Bifidobacterium strains in monocultures and co-cultures (pairs and consortium) were used in this model to evaluate the effect of TMIF on the viability and metabolic activity of those bacteria. The optimization of the in vitro model of the gut microbiota was successful, and growth of the most important groups of bacteria in the gut microbiota was observed. So this model can be used to study the effects of other ingredients at that level. It also enabled pinpointing the prebiotic effects of the studied TMIF suggesting possible symbiotic interactions. In addition, the direct effect of the TMIF on bacterial cells, when in nutritive stress was also evaluated. In terms of TMIF effects on probiotic bacteria viability and growth, no negative effects were observed, and even an enhancement of growth and an increase of production of short chain fatty acids (SCFA) and lactate, in most of the cases, was observed. Also, this study showed that TMIF helps in maintaining the viability of bacteria during incubation time when these are under nutritional stress conditions. A potential prebiotic effect of TMIF is then predictable with this study, opening a path to new research on this subject.Royal Society of ChemistryVeritati - Repositório Institucional da Universidade Católica PortuguesaCarvalho, Nelson Mota deTeixeira, FranciscoSilva, SaraMadureira, Ana RaquelPintado, Manuela Estevez2019-05-14T17:20:33Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/27650engCarvalho, N. M. de, Teixeira, F., Silva, S., Madureira, A. R., & Pintado, M. E. (2019). Potential prebiotic activity of Tenebrio molitor insect flour using an optimized in vitro gut microbiota model. Food & Function, 10(7), 3909–3922. https://doi.org/10.1039/c8fo01536h2042-649610.1039/c8fo01536h2042-650X8506946924631192321000476683600007info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-10T01:39:36Zoai:repositorio.ucp.pt:10400.14/27650Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:22:07.281544Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Potential prebiotic activity of Tenebrio molitor insect flour using an optimized in vitro gut microbiota model |
title |
Potential prebiotic activity of Tenebrio molitor insect flour using an optimized in vitro gut microbiota model |
spellingShingle |
Potential prebiotic activity of Tenebrio molitor insect flour using an optimized in vitro gut microbiota model Carvalho, Nelson Mota de |
title_short |
Potential prebiotic activity of Tenebrio molitor insect flour using an optimized in vitro gut microbiota model |
title_full |
Potential prebiotic activity of Tenebrio molitor insect flour using an optimized in vitro gut microbiota model |
title_fullStr |
Potential prebiotic activity of Tenebrio molitor insect flour using an optimized in vitro gut microbiota model |
title_full_unstemmed |
Potential prebiotic activity of Tenebrio molitor insect flour using an optimized in vitro gut microbiota model |
title_sort |
Potential prebiotic activity of Tenebrio molitor insect flour using an optimized in vitro gut microbiota model |
author |
Carvalho, Nelson Mota de |
author_facet |
Carvalho, Nelson Mota de Teixeira, Francisco Silva, Sara Madureira, Ana Raquel Pintado, Manuela Estevez |
author_role |
author |
author2 |
Teixeira, Francisco Silva, Sara Madureira, Ana Raquel Pintado, Manuela Estevez |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Carvalho, Nelson Mota de Teixeira, Francisco Silva, Sara Madureira, Ana Raquel Pintado, Manuela Estevez |
description |
The Tenebrio molitor insect flour (TMIF) is considered a nutritive food ingredient, but its impact at the gut microbiota level and its potential prebiotic activity still need be assessed. For such studies, an in vitro simulation model of gut microbiota was optimized. Pure cultures of Lactobacillus and Bifidobacterium strains in monocultures and co-cultures (pairs and consortium) were used in this model to evaluate the effect of TMIF on the viability and metabolic activity of those bacteria. The optimization of the in vitro model of the gut microbiota was successful, and growth of the most important groups of bacteria in the gut microbiota was observed. So this model can be used to study the effects of other ingredients at that level. It also enabled pinpointing the prebiotic effects of the studied TMIF suggesting possible symbiotic interactions. In addition, the direct effect of the TMIF on bacterial cells, when in nutritive stress was also evaluated. In terms of TMIF effects on probiotic bacteria viability and growth, no negative effects were observed, and even an enhancement of growth and an increase of production of short chain fatty acids (SCFA) and lactate, in most of the cases, was observed. Also, this study showed that TMIF helps in maintaining the viability of bacteria during incubation time when these are under nutritional stress conditions. A potential prebiotic effect of TMIF is then predictable with this study, opening a path to new research on this subject. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-05-14T17:20:33Z 2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/27650 |
url |
http://hdl.handle.net/10400.14/27650 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Carvalho, N. M. de, Teixeira, F., Silva, S., Madureira, A. R., & Pintado, M. E. (2019). Potential prebiotic activity of Tenebrio molitor insect flour using an optimized in vitro gut microbiota model. Food & Function, 10(7), 3909–3922. https://doi.org/10.1039/c8fo01536h 2042-6496 10.1039/c8fo01536h 2042-650X 85069469246 31192321 000476683600007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131925063925760 |