Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier

Detalhes bibliográficos
Autor(a) principal: Mendes-Jorge, Luísa
Data de Publicação: 2009
Outros Autores: Ramosspi-Sup, David, Luppo, Mariana, Llombart, Cristina, Alexandre-Pires, Graca, Nacher, Victor, Melgarejo, Veronica, Correia, Miguel, Carretero, Ana, Tafurospi-Sup, Sabrina, Rodriguez-Baeza, Alfonso, Pina, José Esperança, Bosch, Fatima, Ruberte, Jesus
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1167/iovs.09-3515
Resumo: The retina contains two distinct populations of mono-cyte-derived cells: perivascular macrophages, and microglia. The present study was undertaken to evaluate the presence and function in mouse and human retinas of a subtype of resident perivascular macrophages with scavenger function, different from microglia, in physiological conditions and during retinopathy. Perivascular macrophages were characterized by means of confocal microscopy, electron microscopy, and flow cytometry analyses. Two murine models of blood-retinal barrier breakdown and photoreceptor degeneration were used to analyze the role of these macrophages during retinopathy. Results. The macrophages analyzed constituted a small population of resident perivascular cells different from microglia, since they were Iba-1 negative. Although these cells expressed F4/80 and CD11b antigens in common with microglia, they also expressed BM8 and MOMA-2 epitopes, which are mac-rophagic markers not expressed by microglia. Perivascular macrophages emitted autofluorescence due to cytoplasmic inclusions containing protein-bound oxidized lipids. They con-stitutively expressed the scavenger receptor class A and moved along blood vessels, providing an additional coating to thinner areas of the basement membrane. Moreover, they accumulated blood-borne horseradish peroxidase and acetylated low-density. lipoprotein in healthy retinas. In addition, during blood-retinal barrier breakdown and photoreceptor degeneration, these cells migrated to the lesion site. Conclusions. All these morphologic and functional features are consistent with those described for brain Mato cells. Thus, this study showed the presence of autofluorescent perivascular macrophages, different from microglia, with a scavenger function that may contribute to the maintenance of the blood- retinal barrier in healthy conditions and that are also involved in retinopathy.
id RCAP_c3b101ae975322271f7ad838a608d8d9
oai_identifier_str oai:run.unl.pt:10362/22029
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrierOphthalmologySensory SystemsCellular and Molecular NeuroscienceMedicine(all)SDG 3 - Good Health and Well-beingThe retina contains two distinct populations of mono-cyte-derived cells: perivascular macrophages, and microglia. The present study was undertaken to evaluate the presence and function in mouse and human retinas of a subtype of resident perivascular macrophages with scavenger function, different from microglia, in physiological conditions and during retinopathy. Perivascular macrophages were characterized by means of confocal microscopy, electron microscopy, and flow cytometry analyses. Two murine models of blood-retinal barrier breakdown and photoreceptor degeneration were used to analyze the role of these macrophages during retinopathy. Results. The macrophages analyzed constituted a small population of resident perivascular cells different from microglia, since they were Iba-1 negative. Although these cells expressed F4/80 and CD11b antigens in common with microglia, they also expressed BM8 and MOMA-2 epitopes, which are mac-rophagic markers not expressed by microglia. Perivascular macrophages emitted autofluorescence due to cytoplasmic inclusions containing protein-bound oxidized lipids. They con-stitutively expressed the scavenger receptor class A and moved along blood vessels, providing an additional coating to thinner areas of the basement membrane. Moreover, they accumulated blood-borne horseradish peroxidase and acetylated low-density. lipoprotein in healthy retinas. In addition, during blood-retinal barrier breakdown and photoreceptor degeneration, these cells migrated to the lesion site. Conclusions. All these morphologic and functional features are consistent with those described for brain Mato cells. Thus, this study showed the presence of autofluorescent perivascular macrophages, different from microglia, with a scavenger function that may contribute to the maintenance of the blood- retinal barrier in healthy conditions and that are also involved in retinopathy.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNMendes-Jorge, LuísaRamosspi-Sup, DavidLuppo, MarianaLlombart, CristinaAlexandre-Pires, GracaNacher, VictorMelgarejo, VeronicaCorreia, MiguelCarretero, AnaTafurospi-Sup, SabrinaRodriguez-Baeza, AlfonsoPina, José EsperançaBosch, FatimaRuberte, Jesus2017-07-17T22:02:00Z2009-122009-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttps://doi.org/10.1167/iovs.09-3515eng0146-0404PURE: 2936350http://www.scopus.com/inward/record.url?scp=73349101416&partnerID=8YFLogxKhttps://doi.org/10.1167/iovs.09-3515info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:26:42Zoai:run.unl.pt:10362/22029Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:26:42Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier
title Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier
spellingShingle Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier
Mendes-Jorge, Luísa
Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience
Medicine(all)
SDG 3 - Good Health and Well-being
title_short Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier
title_full Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier
title_fullStr Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier
title_full_unstemmed Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier
title_sort Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier
author Mendes-Jorge, Luísa
author_facet Mendes-Jorge, Luísa
Ramosspi-Sup, David
Luppo, Mariana
Llombart, Cristina
Alexandre-Pires, Graca
Nacher, Victor
Melgarejo, Veronica
Correia, Miguel
Carretero, Ana
Tafurospi-Sup, Sabrina
Rodriguez-Baeza, Alfonso
Pina, José Esperança
Bosch, Fatima
Ruberte, Jesus
author_role author
author2 Ramosspi-Sup, David
Luppo, Mariana
Llombart, Cristina
Alexandre-Pires, Graca
Nacher, Victor
Melgarejo, Veronica
Correia, Miguel
Carretero, Ana
Tafurospi-Sup, Sabrina
Rodriguez-Baeza, Alfonso
Pina, José Esperança
Bosch, Fatima
Ruberte, Jesus
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Mendes-Jorge, Luísa
Ramosspi-Sup, David
Luppo, Mariana
Llombart, Cristina
Alexandre-Pires, Graca
Nacher, Victor
Melgarejo, Veronica
Correia, Miguel
Carretero, Ana
Tafurospi-Sup, Sabrina
Rodriguez-Baeza, Alfonso
Pina, José Esperança
Bosch, Fatima
Ruberte, Jesus
dc.subject.por.fl_str_mv Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience
Medicine(all)
SDG 3 - Good Health and Well-being
topic Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience
Medicine(all)
SDG 3 - Good Health and Well-being
description The retina contains two distinct populations of mono-cyte-derived cells: perivascular macrophages, and microglia. The present study was undertaken to evaluate the presence and function in mouse and human retinas of a subtype of resident perivascular macrophages with scavenger function, different from microglia, in physiological conditions and during retinopathy. Perivascular macrophages were characterized by means of confocal microscopy, electron microscopy, and flow cytometry analyses. Two murine models of blood-retinal barrier breakdown and photoreceptor degeneration were used to analyze the role of these macrophages during retinopathy. Results. The macrophages analyzed constituted a small population of resident perivascular cells different from microglia, since they were Iba-1 negative. Although these cells expressed F4/80 and CD11b antigens in common with microglia, they also expressed BM8 and MOMA-2 epitopes, which are mac-rophagic markers not expressed by microglia. Perivascular macrophages emitted autofluorescence due to cytoplasmic inclusions containing protein-bound oxidized lipids. They con-stitutively expressed the scavenger receptor class A and moved along blood vessels, providing an additional coating to thinner areas of the basement membrane. Moreover, they accumulated blood-borne horseradish peroxidase and acetylated low-density. lipoprotein in healthy retinas. In addition, during blood-retinal barrier breakdown and photoreceptor degeneration, these cells migrated to the lesion site. Conclusions. All these morphologic and functional features are consistent with those described for brain Mato cells. Thus, this study showed the presence of autofluorescent perivascular macrophages, different from microglia, with a scavenger function that may contribute to the maintenance of the blood- retinal barrier in healthy conditions and that are also involved in retinopathy.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
2009-12-01T00:00:00Z
2017-07-17T22:02:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1167/iovs.09-3515
url https://doi.org/10.1167/iovs.09-3515
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0146-0404
PURE: 2936350
http://www.scopus.com/inward/record.url?scp=73349101416&partnerID=8YFLogxK
https://doi.org/10.1167/iovs.09-3515
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817545594692960256