Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1167/iovs.09-3515 |
Resumo: | The retina contains two distinct populations of mono-cyte-derived cells: perivascular macrophages, and microglia. The present study was undertaken to evaluate the presence and function in mouse and human retinas of a subtype of resident perivascular macrophages with scavenger function, different from microglia, in physiological conditions and during retinopathy. Perivascular macrophages were characterized by means of confocal microscopy, electron microscopy, and flow cytometry analyses. Two murine models of blood-retinal barrier breakdown and photoreceptor degeneration were used to analyze the role of these macrophages during retinopathy. Results. The macrophages analyzed constituted a small population of resident perivascular cells different from microglia, since they were Iba-1 negative. Although these cells expressed F4/80 and CD11b antigens in common with microglia, they also expressed BM8 and MOMA-2 epitopes, which are mac-rophagic markers not expressed by microglia. Perivascular macrophages emitted autofluorescence due to cytoplasmic inclusions containing protein-bound oxidized lipids. They con-stitutively expressed the scavenger receptor class A and moved along blood vessels, providing an additional coating to thinner areas of the basement membrane. Moreover, they accumulated blood-borne horseradish peroxidase and acetylated low-density. lipoprotein in healthy retinas. In addition, during blood-retinal barrier breakdown and photoreceptor degeneration, these cells migrated to the lesion site. Conclusions. All these morphologic and functional features are consistent with those described for brain Mato cells. Thus, this study showed the presence of autofluorescent perivascular macrophages, different from microglia, with a scavenger function that may contribute to the maintenance of the blood- retinal barrier in healthy conditions and that are also involved in retinopathy. |
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Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrierOphthalmologySensory SystemsCellular and Molecular NeuroscienceMedicine(all)SDG 3 - Good Health and Well-beingThe retina contains two distinct populations of mono-cyte-derived cells: perivascular macrophages, and microglia. The present study was undertaken to evaluate the presence and function in mouse and human retinas of a subtype of resident perivascular macrophages with scavenger function, different from microglia, in physiological conditions and during retinopathy. Perivascular macrophages were characterized by means of confocal microscopy, electron microscopy, and flow cytometry analyses. Two murine models of blood-retinal barrier breakdown and photoreceptor degeneration were used to analyze the role of these macrophages during retinopathy. Results. The macrophages analyzed constituted a small population of resident perivascular cells different from microglia, since they were Iba-1 negative. Although these cells expressed F4/80 and CD11b antigens in common with microglia, they also expressed BM8 and MOMA-2 epitopes, which are mac-rophagic markers not expressed by microglia. Perivascular macrophages emitted autofluorescence due to cytoplasmic inclusions containing protein-bound oxidized lipids. They con-stitutively expressed the scavenger receptor class A and moved along blood vessels, providing an additional coating to thinner areas of the basement membrane. Moreover, they accumulated blood-borne horseradish peroxidase and acetylated low-density. lipoprotein in healthy retinas. In addition, during blood-retinal barrier breakdown and photoreceptor degeneration, these cells migrated to the lesion site. Conclusions. All these morphologic and functional features are consistent with those described for brain Mato cells. Thus, this study showed the presence of autofluorescent perivascular macrophages, different from microglia, with a scavenger function that may contribute to the maintenance of the blood- retinal barrier in healthy conditions and that are also involved in retinopathy.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNMendes-Jorge, LuísaRamosspi-Sup, DavidLuppo, MarianaLlombart, CristinaAlexandre-Pires, GracaNacher, VictorMelgarejo, VeronicaCorreia, MiguelCarretero, AnaTafurospi-Sup, SabrinaRodriguez-Baeza, AlfonsoPina, José EsperançaBosch, FatimaRuberte, Jesus2017-07-17T22:02:00Z2009-122009-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttps://doi.org/10.1167/iovs.09-3515eng0146-0404PURE: 2936350http://www.scopus.com/inward/record.url?scp=73349101416&partnerID=8YFLogxKhttps://doi.org/10.1167/iovs.09-3515info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:26:42Zoai:run.unl.pt:10362/22029Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:26:42Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier |
title |
Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier |
spellingShingle |
Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier Mendes-Jorge, Luísa Ophthalmology Sensory Systems Cellular and Molecular Neuroscience Medicine(all) SDG 3 - Good Health and Well-being |
title_short |
Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier |
title_full |
Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier |
title_fullStr |
Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier |
title_full_unstemmed |
Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier |
title_sort |
Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier |
author |
Mendes-Jorge, Luísa |
author_facet |
Mendes-Jorge, Luísa Ramosspi-Sup, David Luppo, Mariana Llombart, Cristina Alexandre-Pires, Graca Nacher, Victor Melgarejo, Veronica Correia, Miguel Carretero, Ana Tafurospi-Sup, Sabrina Rodriguez-Baeza, Alfonso Pina, José Esperança Bosch, Fatima Ruberte, Jesus |
author_role |
author |
author2 |
Ramosspi-Sup, David Luppo, Mariana Llombart, Cristina Alexandre-Pires, Graca Nacher, Victor Melgarejo, Veronica Correia, Miguel Carretero, Ana Tafurospi-Sup, Sabrina Rodriguez-Baeza, Alfonso Pina, José Esperança Bosch, Fatima Ruberte, Jesus |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
dc.contributor.author.fl_str_mv |
Mendes-Jorge, Luísa Ramosspi-Sup, David Luppo, Mariana Llombart, Cristina Alexandre-Pires, Graca Nacher, Victor Melgarejo, Veronica Correia, Miguel Carretero, Ana Tafurospi-Sup, Sabrina Rodriguez-Baeza, Alfonso Pina, José Esperança Bosch, Fatima Ruberte, Jesus |
dc.subject.por.fl_str_mv |
Ophthalmology Sensory Systems Cellular and Molecular Neuroscience Medicine(all) SDG 3 - Good Health and Well-being |
topic |
Ophthalmology Sensory Systems Cellular and Molecular Neuroscience Medicine(all) SDG 3 - Good Health and Well-being |
description |
The retina contains two distinct populations of mono-cyte-derived cells: perivascular macrophages, and microglia. The present study was undertaken to evaluate the presence and function in mouse and human retinas of a subtype of resident perivascular macrophages with scavenger function, different from microglia, in physiological conditions and during retinopathy. Perivascular macrophages were characterized by means of confocal microscopy, electron microscopy, and flow cytometry analyses. Two murine models of blood-retinal barrier breakdown and photoreceptor degeneration were used to analyze the role of these macrophages during retinopathy. Results. The macrophages analyzed constituted a small population of resident perivascular cells different from microglia, since they were Iba-1 negative. Although these cells expressed F4/80 and CD11b antigens in common with microglia, they also expressed BM8 and MOMA-2 epitopes, which are mac-rophagic markers not expressed by microglia. Perivascular macrophages emitted autofluorescence due to cytoplasmic inclusions containing protein-bound oxidized lipids. They con-stitutively expressed the scavenger receptor class A and moved along blood vessels, providing an additional coating to thinner areas of the basement membrane. Moreover, they accumulated blood-borne horseradish peroxidase and acetylated low-density. lipoprotein in healthy retinas. In addition, during blood-retinal barrier breakdown and photoreceptor degeneration, these cells migrated to the lesion site. Conclusions. All these morphologic and functional features are consistent with those described for brain Mato cells. Thus, this study showed the presence of autofluorescent perivascular macrophages, different from microglia, with a scavenger function that may contribute to the maintenance of the blood- retinal barrier in healthy conditions and that are also involved in retinopathy. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-12 2009-12-01T00:00:00Z 2017-07-17T22:02:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1167/iovs.09-3515 |
url |
https://doi.org/10.1167/iovs.09-3515 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0146-0404 PURE: 2936350 http://www.scopus.com/inward/record.url?scp=73349101416&partnerID=8YFLogxK https://doi.org/10.1167/iovs.09-3515 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817545594692960256 |