Evaluation of in vitro solar protection factor (SPF), antioxidant activity, and cell viability of mixed vegetable extracts from Dirmophandra mollis benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/61853 |
Resumo: | The aim of this study was to validate a HPLC method for the assay of flavonoids in extracts obtained from natural sources, i.e., from Dirmophandra mollis Benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L. The potential sun protecting effect, antioxidant activity, and cell viability of the extracts were also determined. Individual extracts (obtained from each individual species) and a mixed extract (containing the four extracts) were analyzed by the validated HPLC method for the identification of flavonoids and quantification of rutin and quercetin. An in vitro cell viability study was carried out using the neutral red method. The in vitro sun protection factor was determined by spectral transmittance and in vitro antioxidant efficacy was evaluated against DPPH, ABTS, and AAPH radicals. The HPLC method used for the identification and quantification of flavonoids in extracts exhibited linearity, precision, accuracy, and robustness. Detection and quantification limits were, respectively, 2.881 ± 0.9 g·mL1 and 0.864 ± 0.9 g·mL1 for quercetin, and 30.09 ± 1 g·mL1 and 9.027 ± 1.1 g·mL1 for rutin. All extracts did not affect cell viability at the evaluated concentration range and exhibited a sun protection effect and antioxidant activity. Among the evaluated extracts, Ginkgo biloba L. and the mixed extract depicted the most expressive antioxidant activity. The mixed extract exhibited sunscreen protection against ultraviolet A (UVA) and ultraviolet B (UVB) and a critical wavelength of 372.7 ± 0.1. Our results translate the enhanced flavonoids composition of the mixed extract, which may be a potential alternative over sunscreens and antioxidants in pharmaceutic/cosmetic formulations. |
id |
RCAP_c3d8ce13a81cd9c67c6d663bebbbff8a |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/61853 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Evaluation of in vitro solar protection factor (SPF), antioxidant activity, and cell viability of mixed vegetable extracts from Dirmophandra mollis benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L.antioxidantcell viabilityflavonoidsquercetinrutinsun protection factorScience & TechnologyThe aim of this study was to validate a HPLC method for the assay of flavonoids in extracts obtained from natural sources, i.e., from Dirmophandra mollis Benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L. The potential sun protecting effect, antioxidant activity, and cell viability of the extracts were also determined. Individual extracts (obtained from each individual species) and a mixed extract (containing the four extracts) were analyzed by the validated HPLC method for the identification of flavonoids and quantification of rutin and quercetin. An in vitro cell viability study was carried out using the neutral red method. The in vitro sun protection factor was determined by spectral transmittance and in vitro antioxidant efficacy was evaluated against DPPH, ABTS, and AAPH radicals. The HPLC method used for the identification and quantification of flavonoids in extracts exhibited linearity, precision, accuracy, and robustness. Detection and quantification limits were, respectively, 2.881 ± 0.9 g·mL1 and 0.864 ± 0.9 g·mL1 for quercetin, and 30.09 ± 1 g·mL1 and 9.027 ± 1.1 g·mL1 for rutin. All extracts did not affect cell viability at the evaluated concentration range and exhibited a sun protection effect and antioxidant activity. Among the evaluated extracts, Ginkgo biloba L. and the mixed extract depicted the most expressive antioxidant activity. The mixed extract exhibited sunscreen protection against ultraviolet A (UVA) and ultraviolet B (UVB) and a critical wavelength of 372.7 ± 0.1. Our results translate the enhanced flavonoids composition of the mixed extract, which may be a potential alternative over sunscreens and antioxidants in pharmaceutic/cosmetic formulations.This research was funded by FAPESP (grant numbers 2015/25533-7 and 2017/14757-7), CAPES and CNPq. The authors also received support from the Portuguese Science and Technology Foundation (FCT/MCT) and from European Funds (PRODER/COMPETE) under the project reference M-ERA-NET/0004/2015-PAIRED, co-financed by FEDER, under the Partnership Agreement PT2020, and PhD scholarship (SFRH/BD/130555/2017).info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoCefali, Letícia CaramoriAtaide, Janaina ArtemFernandes, Ana RitaSanchez-Lopez, ElenaSousa, Ilza Maria de OliveiraFigueiredo, Mariana CecchettoRuiz, Ana Lucia Tasca GoisFoglio, Mary AnnMazzola, Priscila GavaSouto, Eliana B.2019-10-262019-10-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/61853engCefali, Letícia Caramori; Ataide, Janaina Artem; Fernandes, Ana Rita; Sanchez-Lopez, Elena; Sousa, Ilza Maria de Oliveira; Figueiredo, Mariana Cecchetto; Ruiz, Ana Lucia Tasca Gois; Foglio, Mary Ann; Mazzola, Priscila Gava; Souto, Eliana, Evaluation of in vitro solar protection factor (SPF), antioxidant activity, and cell viability of mixed vegetable extracts from Dirmophandra mollis benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L.. Plants, 8(11), 20192223-774710.3390/plants8110453https://www.mdpi.com/journal/plantsinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:37:00Zoai:repositorium.sdum.uminho.pt:1822/61853Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:33:13.269848Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluation of in vitro solar protection factor (SPF), antioxidant activity, and cell viability of mixed vegetable extracts from Dirmophandra mollis benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L. |
title |
Evaluation of in vitro solar protection factor (SPF), antioxidant activity, and cell viability of mixed vegetable extracts from Dirmophandra mollis benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L. |
spellingShingle |
Evaluation of in vitro solar protection factor (SPF), antioxidant activity, and cell viability of mixed vegetable extracts from Dirmophandra mollis benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L. Cefali, Letícia Caramori antioxidant cell viability flavonoids quercetin rutin sun protection factor Science & Technology |
title_short |
Evaluation of in vitro solar protection factor (SPF), antioxidant activity, and cell viability of mixed vegetable extracts from Dirmophandra mollis benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L. |
title_full |
Evaluation of in vitro solar protection factor (SPF), antioxidant activity, and cell viability of mixed vegetable extracts from Dirmophandra mollis benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L. |
title_fullStr |
Evaluation of in vitro solar protection factor (SPF), antioxidant activity, and cell viability of mixed vegetable extracts from Dirmophandra mollis benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L. |
title_full_unstemmed |
Evaluation of in vitro solar protection factor (SPF), antioxidant activity, and cell viability of mixed vegetable extracts from Dirmophandra mollis benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L. |
title_sort |
Evaluation of in vitro solar protection factor (SPF), antioxidant activity, and cell viability of mixed vegetable extracts from Dirmophandra mollis benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L. |
author |
Cefali, Letícia Caramori |
author_facet |
Cefali, Letícia Caramori Ataide, Janaina Artem Fernandes, Ana Rita Sanchez-Lopez, Elena Sousa, Ilza Maria de Oliveira Figueiredo, Mariana Cecchetto Ruiz, Ana Lucia Tasca Gois Foglio, Mary Ann Mazzola, Priscila Gava Souto, Eliana B. |
author_role |
author |
author2 |
Ataide, Janaina Artem Fernandes, Ana Rita Sanchez-Lopez, Elena Sousa, Ilza Maria de Oliveira Figueiredo, Mariana Cecchetto Ruiz, Ana Lucia Tasca Gois Foglio, Mary Ann Mazzola, Priscila Gava Souto, Eliana B. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Cefali, Letícia Caramori Ataide, Janaina Artem Fernandes, Ana Rita Sanchez-Lopez, Elena Sousa, Ilza Maria de Oliveira Figueiredo, Mariana Cecchetto Ruiz, Ana Lucia Tasca Gois Foglio, Mary Ann Mazzola, Priscila Gava Souto, Eliana B. |
dc.subject.por.fl_str_mv |
antioxidant cell viability flavonoids quercetin rutin sun protection factor Science & Technology |
topic |
antioxidant cell viability flavonoids quercetin rutin sun protection factor Science & Technology |
description |
The aim of this study was to validate a HPLC method for the assay of flavonoids in extracts obtained from natural sources, i.e., from Dirmophandra mollis Benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L. The potential sun protecting effect, antioxidant activity, and cell viability of the extracts were also determined. Individual extracts (obtained from each individual species) and a mixed extract (containing the four extracts) were analyzed by the validated HPLC method for the identification of flavonoids and quantification of rutin and quercetin. An in vitro cell viability study was carried out using the neutral red method. The in vitro sun protection factor was determined by spectral transmittance and in vitro antioxidant efficacy was evaluated against DPPH, ABTS, and AAPH radicals. The HPLC method used for the identification and quantification of flavonoids in extracts exhibited linearity, precision, accuracy, and robustness. Detection and quantification limits were, respectively, 2.881 ± 0.9 g·mL1 and 0.864 ± 0.9 g·mL1 for quercetin, and 30.09 ± 1 g·mL1 and 9.027 ± 1.1 g·mL1 for rutin. All extracts did not affect cell viability at the evaluated concentration range and exhibited a sun protection effect and antioxidant activity. Among the evaluated extracts, Ginkgo biloba L. and the mixed extract depicted the most expressive antioxidant activity. The mixed extract exhibited sunscreen protection against ultraviolet A (UVA) and ultraviolet B (UVB) and a critical wavelength of 372.7 ± 0.1. Our results translate the enhanced flavonoids composition of the mixed extract, which may be a potential alternative over sunscreens and antioxidants in pharmaceutic/cosmetic formulations. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-26 2019-10-26T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/61853 |
url |
http://hdl.handle.net/1822/61853 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cefali, Letícia Caramori; Ataide, Janaina Artem; Fernandes, Ana Rita; Sanchez-Lopez, Elena; Sousa, Ilza Maria de Oliveira; Figueiredo, Mariana Cecchetto; Ruiz, Ana Lucia Tasca Gois; Foglio, Mary Ann; Mazzola, Priscila Gava; Souto, Eliana, Evaluation of in vitro solar protection factor (SPF), antioxidant activity, and cell viability of mixed vegetable extracts from Dirmophandra mollis benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L.. Plants, 8(11), 2019 2223-7747 10.3390/plants8110453 https://www.mdpi.com/journal/plants |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132848917053440 |