An amputation resets positional information to a proximal identity in the regenerating zebrafish caudal fin

Detalhes bibliográficos
Autor(a) principal: Azevedo, Ana Sofia
Data de Publicação: 2012
Outros Autores: Sousa, Sara, Jacinto, António, Saúde, Leonor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/23432
Resumo: We thank Lara Carvalho and Fabio Valerio for excellent zebrafish husbandry, Andreia Pinto for histology and Antonio Temudo for imaging support. We are grateful to C. Certal and S. Pascoal for comments on the manuscript. A. S. A and S. S were supported by FCT fellowships (SFRH/BD/33179/2007 and SFER/BD/32952/2006). L. S. was supported by two FCT grants (PTDC/SAU-OBD/64628/2006 and PTDC/SAU-OBD/100202/2008) and A.J. was supported by one FCT grant (PTDC/SAU-OBD/100200/2008).
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spelling An amputation resets positional information to a proximal identity in the regenerating zebrafish caudal finPROLIFERATIONLIMB REGENERATIONBony ray bifurcationsRETINOIC ACIDSONIC HEDGEHOGBLASTEMAFgfZebrafishShhCaudal fin regenerationGROWTHCaudal fin regenerationBony ray bifurcationsShh, FgfZebrafishWe thank Lara Carvalho and Fabio Valerio for excellent zebrafish husbandry, Andreia Pinto for histology and Antonio Temudo for imaging support. We are grateful to C. Certal and S. Pascoal for comments on the manuscript. A. S. A and S. S were supported by FCT fellowships (SFRH/BD/33179/2007 and SFER/BD/32952/2006). L. S. was supported by two FCT grants (PTDC/SAU-OBD/64628/2006 and PTDC/SAU-OBD/100202/2008) and A.J. was supported by one FCT grant (PTDC/SAU-OBD/100200/2008).Background: Zebrafish has emerged as a powerful model organism to study the process of regeneration. This teleost fish has the ability to regenerate various tissues and organs like the heart, spinal cord, retina and fins. In this study, we took advantage of the existence of an excellent morphological reference in the zebrafish caudal fin, the bony ray bifurcations, as a model to study positional information upon amputation. We investigated the existence of positional information for bifurcation formation by performing repeated amputations at different proximal-distal places along the fin. Results: We show that, while amputations performed at a long distance from the bifurcation do not change its final proximal-distal position in the regenerated fin, consecutive amputations done at 1 segment proximal to the bifurcation (near the bifurcation) induce a positional reset and progressively shift its position distally. Furthermore, we investigated the potential role of Shh and Fgf signalling pathways in the determination of the bifurcation position and observed that they do not seem to be involved in this process. Conclusions: Our results reveal that, an amputation near the bifurcation inhibits the formation of the regenerated bifurcation in the pre-amputation position, inducing a distalization of this structure. This shows that the positional memory for bony ray bifurcations depends on the proximal-distal level of the amputation.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNAzevedo, Ana SofiaSousa, SaraJacinto, AntónioSaúde, Leonor2017-09-19T22:00:33Z2012-08-252012-08-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://hdl.handle.net/10362/23432eng1471-213XPURE: 148981https://doi.org/10.1186/1471-213X-12-24info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:11:42Zoai:run.unl.pt:10362/23432Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:27:47.641576Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv An amputation resets positional information to a proximal identity in the regenerating zebrafish caudal fin
title An amputation resets positional information to a proximal identity in the regenerating zebrafish caudal fin
spellingShingle An amputation resets positional information to a proximal identity in the regenerating zebrafish caudal fin
Azevedo, Ana Sofia
PROLIFERATION
LIMB REGENERATION
Bony ray bifurcations
RETINOIC ACID
SONIC HEDGEHOG
BLASTEMA
Fgf
Zebrafish
Shh
Caudal fin regeneration
GROWTH
Caudal fin regeneration
Bony ray bifurcations
Shh, Fgf
Zebrafish
title_short An amputation resets positional information to a proximal identity in the regenerating zebrafish caudal fin
title_full An amputation resets positional information to a proximal identity in the regenerating zebrafish caudal fin
title_fullStr An amputation resets positional information to a proximal identity in the regenerating zebrafish caudal fin
title_full_unstemmed An amputation resets positional information to a proximal identity in the regenerating zebrafish caudal fin
title_sort An amputation resets positional information to a proximal identity in the regenerating zebrafish caudal fin
author Azevedo, Ana Sofia
author_facet Azevedo, Ana Sofia
Sousa, Sara
Jacinto, António
Saúde, Leonor
author_role author
author2 Sousa, Sara
Jacinto, António
Saúde, Leonor
author2_role author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Azevedo, Ana Sofia
Sousa, Sara
Jacinto, António
Saúde, Leonor
dc.subject.por.fl_str_mv PROLIFERATION
LIMB REGENERATION
Bony ray bifurcations
RETINOIC ACID
SONIC HEDGEHOG
BLASTEMA
Fgf
Zebrafish
Shh
Caudal fin regeneration
GROWTH
Caudal fin regeneration
Bony ray bifurcations
Shh, Fgf
Zebrafish
topic PROLIFERATION
LIMB REGENERATION
Bony ray bifurcations
RETINOIC ACID
SONIC HEDGEHOG
BLASTEMA
Fgf
Zebrafish
Shh
Caudal fin regeneration
GROWTH
Caudal fin regeneration
Bony ray bifurcations
Shh, Fgf
Zebrafish
description We thank Lara Carvalho and Fabio Valerio for excellent zebrafish husbandry, Andreia Pinto for histology and Antonio Temudo for imaging support. We are grateful to C. Certal and S. Pascoal for comments on the manuscript. A. S. A and S. S were supported by FCT fellowships (SFRH/BD/33179/2007 and SFER/BD/32952/2006). L. S. was supported by two FCT grants (PTDC/SAU-OBD/64628/2006 and PTDC/SAU-OBD/100202/2008) and A.J. was supported by one FCT grant (PTDC/SAU-OBD/100200/2008).
publishDate 2012
dc.date.none.fl_str_mv 2012-08-25
2012-08-25T00:00:00Z
2017-09-19T22:00:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/23432
url http://hdl.handle.net/10362/23432
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1471-213X
PURE: 148981
https://doi.org/10.1186/1471-213X-12-24
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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