Patterning in time and space: HoxB cluster gene expression in the developing chick embryo
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/62234 |
Resumo: | The developing embryo is a paradigmatic model to study molecular mechanisms of time control in Biology. Hox genes are key players in the specification of tissue identity during embryo development and their expression is under strict temporal regulation. However, the molecular mechanisms underlying timely Hox activation in the early embryo remain unknown. This is hindered by the lack of a rigorous temporal framework of sequential Hox expression within a single cluster. Herein, a thorough characterization of HoxB cluster gene expression was performed over time and space in the early chick embryo. Clear temporal collinearity of HoxB cluster gene expression activation was observed. Spatial collinearity of HoxB expression was evidenced in different stages of development and in multiple tissues. Using embryo explant cultures we showed that HoxB2 is cyclically expressed in the rostral presomitic mesoderm with the same periodicity as somite formation, suggesting a link between timely tissue specification and somite formation. We foresee that the molecular framework herein provided will facilitate experimental approaches aimed at identifying the regulatory mechanisms underlying Hox expression in Time and Space. |
id |
RCAP_c49a952d9c4805a462aa4829b4f8fb18 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/62234 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Patterning in time and space: HoxB cluster gene expression in the developing chick embryoAnimalsChick EmbryoChickensEmbryonic DevelopmentGene Expression Regulation, DevelopmentalHomeodomain ProteinsIn Situ HybridizationMesodermHox genesHox codespatial collinearitytemporal collinearitytemporal controlCiências Médicas::Medicina BásicaScience & TechnologyThe developing embryo is a paradigmatic model to study molecular mechanisms of time control in Biology. Hox genes are key players in the specification of tissue identity during embryo development and their expression is under strict temporal regulation. However, the molecular mechanisms underlying timely Hox activation in the early embryo remain unknown. This is hindered by the lack of a rigorous temporal framework of sequential Hox expression within a single cluster. Herein, a thorough characterization of HoxB cluster gene expression was performed over time and space in the early chick embryo. Clear temporal collinearity of HoxB cluster gene expression activation was observed. Spatial collinearity of HoxB expression was evidenced in different stages of development and in multiple tissues. Using embryo explant cultures we showed that HoxB2 is cyclically expressed in the rostral presomitic mesoderm with the same periodicity as somite formation, suggesting a link between timely tissue specification and somite formation. We foresee that the molecular framework herein provided will facilitate experimental approaches aimed at identifying the regulatory mechanisms underlying Hox expression in Time and Space.Fundação para a Ciência e a Tecnologia (FCT), Portugal (grants PTDC/SAU-OBD/105111/2008, UMINHO/BI/7/2014 and SFRH/BPD/65652/2009, respectively). RPA was funded by a Ciencia2007 Program Contract and Programa Operacional Regional do Norte (ON.2) – NORTE-07–0124-FEDER-000017. This work was supported by national Portuguese funding through FCT (National and FEDER COMPETE Program funds: PTDC/SAU-BID/121459/2010 and PTDC/SAU-OBD/099758/2008 to RPA and IP, respectively), and by PEst-OE/EQB/LA0023/2011Taylor & FrancisUniversidade do MinhoGouveia, Analuce CanhaMarcelino, Hugo Miguel RochaGonçalves, LisaPalmeirim, IsabelAndrade, Raquel P.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/62234engGouveia, A., Marcelino, H. M., Gonçalves, L., Palmeirim, I., & Andrade, R. P. (2015). Patterning in time and space: HoxB cluster gene expression in the developing chick embryo. Cell Cycle, 14(1), 135-145.1538-41011551-400510.4161/15384101.2014.97286825602523https://www.tandfonline.com/doi/full/10.4161/15384101.2014.972868info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:43:57Zoai:repositorium.sdum.uminho.pt:1822/62234Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:41:31.181260Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Patterning in time and space: HoxB cluster gene expression in the developing chick embryo |
title |
Patterning in time and space: HoxB cluster gene expression in the developing chick embryo |
spellingShingle |
Patterning in time and space: HoxB cluster gene expression in the developing chick embryo Gouveia, Analuce Canha Animals Chick Embryo Chickens Embryonic Development Gene Expression Regulation, Developmental Homeodomain Proteins In Situ Hybridization Mesoderm Hox genes Hox code spatial collinearity temporal collinearity temporal control Ciências Médicas::Medicina Básica Science & Technology |
title_short |
Patterning in time and space: HoxB cluster gene expression in the developing chick embryo |
title_full |
Patterning in time and space: HoxB cluster gene expression in the developing chick embryo |
title_fullStr |
Patterning in time and space: HoxB cluster gene expression in the developing chick embryo |
title_full_unstemmed |
Patterning in time and space: HoxB cluster gene expression in the developing chick embryo |
title_sort |
Patterning in time and space: HoxB cluster gene expression in the developing chick embryo |
author |
Gouveia, Analuce Canha |
author_facet |
Gouveia, Analuce Canha Marcelino, Hugo Miguel Rocha Gonçalves, Lisa Palmeirim, Isabel Andrade, Raquel P. |
author_role |
author |
author2 |
Marcelino, Hugo Miguel Rocha Gonçalves, Lisa Palmeirim, Isabel Andrade, Raquel P. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Gouveia, Analuce Canha Marcelino, Hugo Miguel Rocha Gonçalves, Lisa Palmeirim, Isabel Andrade, Raquel P. |
dc.subject.por.fl_str_mv |
Animals Chick Embryo Chickens Embryonic Development Gene Expression Regulation, Developmental Homeodomain Proteins In Situ Hybridization Mesoderm Hox genes Hox code spatial collinearity temporal collinearity temporal control Ciências Médicas::Medicina Básica Science & Technology |
topic |
Animals Chick Embryo Chickens Embryonic Development Gene Expression Regulation, Developmental Homeodomain Proteins In Situ Hybridization Mesoderm Hox genes Hox code spatial collinearity temporal collinearity temporal control Ciências Médicas::Medicina Básica Science & Technology |
description |
The developing embryo is a paradigmatic model to study molecular mechanisms of time control in Biology. Hox genes are key players in the specification of tissue identity during embryo development and their expression is under strict temporal regulation. However, the molecular mechanisms underlying timely Hox activation in the early embryo remain unknown. This is hindered by the lack of a rigorous temporal framework of sequential Hox expression within a single cluster. Herein, a thorough characterization of HoxB cluster gene expression was performed over time and space in the early chick embryo. Clear temporal collinearity of HoxB cluster gene expression activation was observed. Spatial collinearity of HoxB expression was evidenced in different stages of development and in multiple tissues. Using embryo explant cultures we showed that HoxB2 is cyclically expressed in the rostral presomitic mesoderm with the same periodicity as somite formation, suggesting a link between timely tissue specification and somite formation. We foresee that the molecular framework herein provided will facilitate experimental approaches aimed at identifying the regulatory mechanisms underlying Hox expression in Time and Space. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/62234 |
url |
http://hdl.handle.net/1822/62234 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gouveia, A., Marcelino, H. M., Gonçalves, L., Palmeirim, I., & Andrade, R. P. (2015). Patterning in time and space: HoxB cluster gene expression in the developing chick embryo. Cell Cycle, 14(1), 135-145. 1538-4101 1551-4005 10.4161/15384101.2014.972868 25602523 https://www.tandfonline.com/doi/full/10.4161/15384101.2014.972868 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis |
publisher.none.fl_str_mv |
Taylor & Francis |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132964627415040 |