Effect of temperature on the reversal of the calcium ion pump in sarcoplasmic reticulum

Detalhes bibliográficos
Autor(a) principal: Vale, M. G. P.
Data de Publicação: 1980
Outros Autores: Carvalho, A. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/13030
https://doi.org/10.1042/bj1860461
Resumo: Biochemical Journal. 186:2 (1980) 461-467
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spelling Effect of temperature on the reversal of the calcium ion pump in sarcoplasmic reticulumBiochemical Journal. 186:2 (1980) 461-467Sarcoplasmic-reticulum vesicles were actively loaded with Ca2+ in the presence of phosphate, and the ADP-induced Ca2+ efflux and ATP synthesis were measured as a function of temperature. Arrhenius plots show break points for both processes at about 18 and 37 degrees C. Between 18 and 37 degrees C, Ca2+ efflux and ATP synthesis occur with an activation energy of 67.2-71.4 kJ/mol, whereas it is about 189-210 kJ/mol for temperatures below 18 degrees C. Above 37 degrees C, the rates of ADP-induced Ca2+ release and of ATP synthesis sharply decline until the temperature reaches about 42 degrees C. Above this temperature, the Ca2+ efflux increases again even in absence of ADP, although the synthesis of ATP is inhibited, which reflects leakiness of the vesicles. The results show that the transition temperatures for ADP-induced Ca2+ efflux and for ATP synthesis resemble those for active Ca2+ uptake, which indicates that the same coupling mechanism is involved during the inward and outward Ca2+ translocations across the membranePortland Press1980-02-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/13030http://hdl.handle.net/10316/13030https://doi.org/10.1042/bj1860461engBiochemical Journal. 186:2 (1980) 461-4670306-3283Vale, M. G. P.Carvalho, A. P.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-11-05T10:55:05Zoai:estudogeral.uc.pt:10316/13030Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:53.485840Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of temperature on the reversal of the calcium ion pump in sarcoplasmic reticulum
title Effect of temperature on the reversal of the calcium ion pump in sarcoplasmic reticulum
spellingShingle Effect of temperature on the reversal of the calcium ion pump in sarcoplasmic reticulum
Vale, M. G. P.
title_short Effect of temperature on the reversal of the calcium ion pump in sarcoplasmic reticulum
title_full Effect of temperature on the reversal of the calcium ion pump in sarcoplasmic reticulum
title_fullStr Effect of temperature on the reversal of the calcium ion pump in sarcoplasmic reticulum
title_full_unstemmed Effect of temperature on the reversal of the calcium ion pump in sarcoplasmic reticulum
title_sort Effect of temperature on the reversal of the calcium ion pump in sarcoplasmic reticulum
author Vale, M. G. P.
author_facet Vale, M. G. P.
Carvalho, A. P.
author_role author
author2 Carvalho, A. P.
author2_role author
dc.contributor.author.fl_str_mv Vale, M. G. P.
Carvalho, A. P.
description Biochemical Journal. 186:2 (1980) 461-467
publishDate 1980
dc.date.none.fl_str_mv 1980-02-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/13030
http://hdl.handle.net/10316/13030
https://doi.org/10.1042/bj1860461
url http://hdl.handle.net/10316/13030
https://doi.org/10.1042/bj1860461
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biochemical Journal. 186:2 (1980) 461-467
0306-3283
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Portland Press
publisher.none.fl_str_mv Portland Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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