Microsatellite variability in natural populations of the blackspot seabream Pagellus bogaraveo (Brunnick, 1768): a database to access parentage assignment in aquaculture

• Autor(a) principal: Lemos, Andreia
• Data de Publicação: 2006
• Outros Autores: Freitas, Ana Isabel, Fernandes, Ana Teresa, Gonçalves, Rita, Jesus, José, Andrade, Carlos, Brehm, António
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.13/3678
• topic: Pagellus bogaraveo; Microsatellites; Genetic structure; Parentage; Database; .; Faculdade de Ciências da Vida

Eight microsatellite loci previously reported were as sessed for their utility in parentage assignment in 96 individuals belonging to natural populations of the blackspot seabream Pagellus bogaraveo (Brˇnnick, 1768) from the Mediterranean and Northeast Atlantic regions. At the mtDNA level, no di¡erentiation was found between these two regions but based on micro satellite data an overall discrete genetic di¡erentiation is perceivable between the two regions separated by the Strait of Gibraltar. The number of alleles per locus ranged from 8 to 30. A database constructed with al lele frequency data from six populations was used in a simulation parentage assignment test using the software CERVUS. The test showed that the number of markers used is enough to perform parentage assign ments with real data. The polymorphic information content for each locus was very high (mean value of 0.849), with a total exclusionary power of 0.9995. In summary, seven of the eight microsatellites analysed proved to be su⁄cient and powerful tools for parent age assignment in hatcheries and the allele frequency data given here can be used to perform pedigree ana lysis against which real data may be tested.