Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids

Detalhes bibliográficos
Autor(a) principal: Palmeira, C. M.
Data de Publicação: 2000
Outros Autores: Rana, M. I., Frederick, C. B., Wallace, K. B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/5447
https://doi.org/10.1006/bbrc.2000.2797
Resumo: We recently reported that acrylic acid (AA) induces the MPT in vitro, which we suggested might be a critical event in the acute inflammatory and hyperplastic response of the olfactory epithelium. The purpose of the present investigation was to determine if induction of the MPT is a general response to short-chain carboxylic acids or if there are critical physical chemical parameters for this response. Freshly isolated rat liver mitochondria were incubated in the presence of varying concentrations of selected carboxylic acids. All of the acids that we tested caused a concentration-dependent induction of the MPT, which was blocked by cyclosporine A. Although the C4 carboxylic acids were slightly more potent than the C5 acids, there was no correlation with the degree of saturation, the octanol/water coefficient (log P), or the dissociation constant (pKa) of the acids that we tested. We conclude that induction of the MPT in vitro is a general response to short-chain carboxylic acids having a pKa of 4 to 5.
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spelling Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acidsliver mitochondria; permeability transition; carboxylic acids; cyclosporine AWe recently reported that acrylic acid (AA) induces the MPT in vitro, which we suggested might be a critical event in the acute inflammatory and hyperplastic response of the olfactory epithelium. The purpose of the present investigation was to determine if induction of the MPT is a general response to short-chain carboxylic acids or if there are critical physical chemical parameters for this response. Freshly isolated rat liver mitochondria were incubated in the presence of varying concentrations of selected carboxylic acids. All of the acids that we tested caused a concentration-dependent induction of the MPT, which was blocked by cyclosporine A. Although the C4 carboxylic acids were slightly more potent than the C5 acids, there was no correlation with the degree of saturation, the octanol/water coefficient (log P), or the dissociation constant (pKa) of the acids that we tested. We conclude that induction of the MPT in vitro is a general response to short-chain carboxylic acids having a pKa of 4 to 5.http://www.sciencedirect.com/science/article/B6WBK-45FC9R7-T/1/dc27a6024b50dcc55a92198672d5618a2000info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5447http://hdl.handle.net/10316/5447https://doi.org/10.1006/bbrc.2000.2797engBiochemical and Biophysical Research Communications. 272:2 (2000) 431-435Palmeira, C. M.Rana, M. I.Frederick, C. B.Wallace, K. B.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-11-10T11:25:29Zoai:estudogeral.uc.pt:10316/5447Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:30.281621Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids
title Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids
spellingShingle Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids
Palmeira, C. M.
liver mitochondria; permeability transition; carboxylic acids; cyclosporine A
title_short Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids
title_full Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids
title_fullStr Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids
title_full_unstemmed Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids
title_sort Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids
author Palmeira, C. M.
author_facet Palmeira, C. M.
Rana, M. I.
Frederick, C. B.
Wallace, K. B.
author_role author
author2 Rana, M. I.
Frederick, C. B.
Wallace, K. B.
author2_role author
author
author
dc.contributor.author.fl_str_mv Palmeira, C. M.
Rana, M. I.
Frederick, C. B.
Wallace, K. B.
dc.subject.por.fl_str_mv liver mitochondria; permeability transition; carboxylic acids; cyclosporine A
topic liver mitochondria; permeability transition; carboxylic acids; cyclosporine A
description We recently reported that acrylic acid (AA) induces the MPT in vitro, which we suggested might be a critical event in the acute inflammatory and hyperplastic response of the olfactory epithelium. The purpose of the present investigation was to determine if induction of the MPT is a general response to short-chain carboxylic acids or if there are critical physical chemical parameters for this response. Freshly isolated rat liver mitochondria were incubated in the presence of varying concentrations of selected carboxylic acids. All of the acids that we tested caused a concentration-dependent induction of the MPT, which was blocked by cyclosporine A. Although the C4 carboxylic acids were slightly more potent than the C5 acids, there was no correlation with the degree of saturation, the octanol/water coefficient (log P), or the dissociation constant (pKa) of the acids that we tested. We conclude that induction of the MPT in vitro is a general response to short-chain carboxylic acids having a pKa of 4 to 5.
publishDate 2000
dc.date.none.fl_str_mv 2000
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/5447
http://hdl.handle.net/10316/5447
https://doi.org/10.1006/bbrc.2000.2797
url http://hdl.handle.net/10316/5447
https://doi.org/10.1006/bbrc.2000.2797
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biochemical and Biophysical Research Communications. 272:2 (2000) 431-435
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dc.format.none.fl_str_mv aplication/PDF
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