Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5447 https://doi.org/10.1006/bbrc.2000.2797 |
Resumo: | We recently reported that acrylic acid (AA) induces the MPT in vitro, which we suggested might be a critical event in the acute inflammatory and hyperplastic response of the olfactory epithelium. The purpose of the present investigation was to determine if induction of the MPT is a general response to short-chain carboxylic acids or if there are critical physical chemical parameters for this response. Freshly isolated rat liver mitochondria were incubated in the presence of varying concentrations of selected carboxylic acids. All of the acids that we tested caused a concentration-dependent induction of the MPT, which was blocked by cyclosporine A. Although the C4 carboxylic acids were slightly more potent than the C5 acids, there was no correlation with the degree of saturation, the octanol/water coefficient (log P), or the dissociation constant (pKa) of the acids that we tested. We conclude that induction of the MPT in vitro is a general response to short-chain carboxylic acids having a pKa of 4 to 5. |
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7160 |
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Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acidsliver mitochondria; permeability transition; carboxylic acids; cyclosporine AWe recently reported that acrylic acid (AA) induces the MPT in vitro, which we suggested might be a critical event in the acute inflammatory and hyperplastic response of the olfactory epithelium. The purpose of the present investigation was to determine if induction of the MPT is a general response to short-chain carboxylic acids or if there are critical physical chemical parameters for this response. Freshly isolated rat liver mitochondria were incubated in the presence of varying concentrations of selected carboxylic acids. All of the acids that we tested caused a concentration-dependent induction of the MPT, which was blocked by cyclosporine A. Although the C4 carboxylic acids were slightly more potent than the C5 acids, there was no correlation with the degree of saturation, the octanol/water coefficient (log P), or the dissociation constant (pKa) of the acids that we tested. We conclude that induction of the MPT in vitro is a general response to short-chain carboxylic acids having a pKa of 4 to 5.http://www.sciencedirect.com/science/article/B6WBK-45FC9R7-T/1/dc27a6024b50dcc55a92198672d5618a2000info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5447http://hdl.handle.net/10316/5447https://doi.org/10.1006/bbrc.2000.2797engBiochemical and Biophysical Research Communications. 272:2 (2000) 431-435Palmeira, C. M.Rana, M. I.Frederick, C. B.Wallace, K. B.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-11-10T11:25:29Zoai:estudogeral.uc.pt:10316/5447Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:30.281621Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids |
title |
Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids |
spellingShingle |
Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids Palmeira, C. M. liver mitochondria; permeability transition; carboxylic acids; cyclosporine A |
title_short |
Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids |
title_full |
Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids |
title_fullStr |
Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids |
title_full_unstemmed |
Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids |
title_sort |
Induction of the Mitochondrial Permeability Transition in Vitro by Short-Chain Carboxylic Acids |
author |
Palmeira, C. M. |
author_facet |
Palmeira, C. M. Rana, M. I. Frederick, C. B. Wallace, K. B. |
author_role |
author |
author2 |
Rana, M. I. Frederick, C. B. Wallace, K. B. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Palmeira, C. M. Rana, M. I. Frederick, C. B. Wallace, K. B. |
dc.subject.por.fl_str_mv |
liver mitochondria; permeability transition; carboxylic acids; cyclosporine A |
topic |
liver mitochondria; permeability transition; carboxylic acids; cyclosporine A |
description |
We recently reported that acrylic acid (AA) induces the MPT in vitro, which we suggested might be a critical event in the acute inflammatory and hyperplastic response of the olfactory epithelium. The purpose of the present investigation was to determine if induction of the MPT is a general response to short-chain carboxylic acids or if there are critical physical chemical parameters for this response. Freshly isolated rat liver mitochondria were incubated in the presence of varying concentrations of selected carboxylic acids. All of the acids that we tested caused a concentration-dependent induction of the MPT, which was blocked by cyclosporine A. Although the C4 carboxylic acids were slightly more potent than the C5 acids, there was no correlation with the degree of saturation, the octanol/water coefficient (log P), or the dissociation constant (pKa) of the acids that we tested. We conclude that induction of the MPT in vitro is a general response to short-chain carboxylic acids having a pKa of 4 to 5. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5447 http://hdl.handle.net/10316/5447 https://doi.org/10.1006/bbrc.2000.2797 |
url |
http://hdl.handle.net/10316/5447 https://doi.org/10.1006/bbrc.2000.2797 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochemical and Biophysical Research Communications. 272:2 (2000) 431-435 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133842171232256 |