The interplay between lncRNAs, RNA-binding proteins and viral genome during SARS-CoV-2 infection reveals strong connections with regulatory events involved in RNA metabolism and immune response
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/150565 |
Resumo: | Funding Information: The authors would like to thank Francisco Enguita Jr. for his valuable insight and help in the design and conceptualization of the manuscript. Publisher Copyright: © The author(s) |
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The interplay between lncRNAs, RNA-binding proteins and viral genome during SARS-CoV-2 infection reveals strong connections with regulatory events involved in RNA metabolism and immune responselong non-coding RNAregulatory networkRNA-binding proteinSARS-CoV-2Medicine (miscellaneous)Pharmacology, Toxicology and Pharmaceutics (miscellaneous)SDG 3 - Good Health and Well-beingFunding Information: The authors would like to thank Francisco Enguita Jr. for his valuable insight and help in the design and conceptualization of the manuscript. Publisher Copyright: © The author(s)Rationale: Viral infections are complex processes based on an intricate network of molecular interactions. The infectious agent hijacks components of the cellular machinery for its profit, circumventing the natural defense mechanisms triggered by the infected cell. The successful completion of the replicative viral cycle within a cell depends on the function of viral components versus the cellular defenses. Non-coding RNAs (ncRNAs) are important cellular modulators, either promoting or preventing the progression of viral infections. Among these ncRNAs, the long non-coding RNA (lncRNA) family is especially relevant due to their intrinsic functional properties and ubiquitous biological roles. Specific lncRNAs have been recently characterized as modulators of the cellular response during infection of human host cells by single stranded RNA viruses. However, the role of host lncRNAs in the infection by human RNA coronaviruses such as SARS-CoV-2 remains uncharacterized. Methods: In the present work, we have performed a transcriptomic study of a cohort of patients with different SARS-CoV-2 viral load and analyzed the involvement of lncRNAs in supporting regulatory networks based on their interaction with RNA-binding proteins (RBPs). Results: Our results revealed the existence of a SARS-CoV-2 infection-dependent pattern of transcriptional up-regulation in which specific lncRNAs are an integral component. To determine the role of these lncRNAs, we performed a functional correlation analysis complemented with the study of the validated interactions between lncRNAs and RBPs. This combination of in silico functional association studies and experimental evidence allowed us to identify a lncRNA signature composed of six elements - NRIR, BISPR, MIR155HG, FMR1-IT1, USP30-AS1, and U62317.2 - associated with the regulation of SARS-CoV-2 infection. Conclusions: We propose a competition mechanism between the viral RNA genome and the regulatory lncRNAs in the sequestering of specific RBPs that modulates the interferon response and the regulation of RNA surveillance by nonsense-mediated decay (NMD).MEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de RecursosDCTB - Departamento de Ciências e Tecnologia da Biomassa (ex-GDEH)RUNEnguita, Francisco J.Leitão, Ana LúciaMcDonald, J. TysonZaksas, ViktorijaDas, SaswatiGaleano, DiegoTaylor, DeanneWurtele, Eve SyrkinSaravia-Butler, AmandaBaylin, Stephen B.Meller, RobertPorterfield, D. MarshallWallace, Douglas C.Schisler, Jonathan C.Mason, Christopher E.Beheshti, Afshin2023-03-14T22:43:47Z2022-05-092022-05-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17application/pdfhttp://hdl.handle.net/10362/150565engPURE: 55667286https://doi.org/10.7150/THNO.73268info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:09:57Zoai:run.unl.pt:10362/150565Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:09:57Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
The interplay between lncRNAs, RNA-binding proteins and viral genome during SARS-CoV-2 infection reveals strong connections with regulatory events involved in RNA metabolism and immune response |
| title |
The interplay between lncRNAs, RNA-binding proteins and viral genome during SARS-CoV-2 infection reveals strong connections with regulatory events involved in RNA metabolism and immune response |
| spellingShingle |
The interplay between lncRNAs, RNA-binding proteins and viral genome during SARS-CoV-2 infection reveals strong connections with regulatory events involved in RNA metabolism and immune response Enguita, Francisco J. long non-coding RNA regulatory network RNA-binding protein SARS-CoV-2 Medicine (miscellaneous) Pharmacology, Toxicology and Pharmaceutics (miscellaneous) SDG 3 - Good Health and Well-being |
| title_short |
The interplay between lncRNAs, RNA-binding proteins and viral genome during SARS-CoV-2 infection reveals strong connections with regulatory events involved in RNA metabolism and immune response |
| title_full |
The interplay between lncRNAs, RNA-binding proteins and viral genome during SARS-CoV-2 infection reveals strong connections with regulatory events involved in RNA metabolism and immune response |
| title_fullStr |
The interplay between lncRNAs, RNA-binding proteins and viral genome during SARS-CoV-2 infection reveals strong connections with regulatory events involved in RNA metabolism and immune response |
| title_full_unstemmed |
The interplay between lncRNAs, RNA-binding proteins and viral genome during SARS-CoV-2 infection reveals strong connections with regulatory events involved in RNA metabolism and immune response |
| title_sort |
The interplay between lncRNAs, RNA-binding proteins and viral genome during SARS-CoV-2 infection reveals strong connections with regulatory events involved in RNA metabolism and immune response |
| author |
Enguita, Francisco J. |
| author_facet |
Enguita, Francisco J. Leitão, Ana Lúcia McDonald, J. Tyson Zaksas, Viktorija Das, Saswati Galeano, Diego Taylor, Deanne Wurtele, Eve Syrkin Saravia-Butler, Amanda Baylin, Stephen B. Meller, Robert Porterfield, D. Marshall Wallace, Douglas C. Schisler, Jonathan C. Mason, Christopher E. Beheshti, Afshin |
| author_role |
author |
| author2 |
Leitão, Ana Lúcia McDonald, J. Tyson Zaksas, Viktorija Das, Saswati Galeano, Diego Taylor, Deanne Wurtele, Eve Syrkin Saravia-Butler, Amanda Baylin, Stephen B. Meller, Robert Porterfield, D. Marshall Wallace, Douglas C. Schisler, Jonathan C. Mason, Christopher E. Beheshti, Afshin |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
MEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de Recursos DCTB - Departamento de Ciências e Tecnologia da Biomassa (ex-GDEH) RUN |
| dc.contributor.author.fl_str_mv |
Enguita, Francisco J. Leitão, Ana Lúcia McDonald, J. Tyson Zaksas, Viktorija Das, Saswati Galeano, Diego Taylor, Deanne Wurtele, Eve Syrkin Saravia-Butler, Amanda Baylin, Stephen B. Meller, Robert Porterfield, D. Marshall Wallace, Douglas C. Schisler, Jonathan C. Mason, Christopher E. Beheshti, Afshin |
| dc.subject.por.fl_str_mv |
long non-coding RNA regulatory network RNA-binding protein SARS-CoV-2 Medicine (miscellaneous) Pharmacology, Toxicology and Pharmaceutics (miscellaneous) SDG 3 - Good Health and Well-being |
| topic |
long non-coding RNA regulatory network RNA-binding protein SARS-CoV-2 Medicine (miscellaneous) Pharmacology, Toxicology and Pharmaceutics (miscellaneous) SDG 3 - Good Health and Well-being |
| description |
Funding Information: The authors would like to thank Francisco Enguita Jr. for his valuable insight and help in the design and conceptualization of the manuscript. Publisher Copyright: © The author(s) |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-05-09 2022-05-09T00:00:00Z 2023-03-14T22:43:47Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/150565 |
| url |
http://hdl.handle.net/10362/150565 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
PURE: 55667286 https://doi.org/10.7150/THNO.73268 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
17 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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mluisa.alvim@gmail.com |
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