Molecular evidence of the close relatedness of clinical, gull and wastewater isolates of quinolone-resistant Escherichia coli

Detalhes bibliográficos
Autor(a) principal: Varela, Ana Rita
Data de Publicação: 2015
Outros Autores: Manageiro, Vera, Ferreira, Eugénia, Guimarães, M. Augusta, da Costa, Paulo Martins, Caniça, Manuela, Manaia, Célia M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/3718
Resumo: Short Communication
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spelling Molecular evidence of the close relatedness of clinical, gull and wastewater isolates of quinolone-resistant Escherichia coliResistência aos AntibióticosQuinolone ResistanceWildlifeWastewaterHospitalMLSTPFGEEscherichia coliShort CommunicationEscherichia coli with reduced susceptibility to quinolones isolated from different environmental sources (urban wastewater treatment plants, n = 61; hospital effluent, n = 10; urban streams, n = 9; gulls, n = 18; birds of prey, n = 17) and from hospitalised patients (n = 28) were compared based on multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). The habitats with the most diversified genotypes of quinolone-resistant E. coli, corresponding to the highest genetic diversity (H′), were wastewater and gulls. In addition, genetically distinct populations were observed in clinical samples and birds of prey, suggesting the influence of the habitat or selective pressures on quinolone-resistant E. coli. The close genetic relatedness between isolates of clinical origin and from gulls and wastewater suggests the existence of potential routes of propagation between these sources. The most common sequence types were ST131 and ST10, with ST131 being highly specific to patients, although distributed in all of the other habitats except birds of prey. The prevalence of antimicrobial resistance was significantly higher in isolates from patients and gulls than from other sources (P < 0.01), suggesting that the effect of selective pressures met by isolates subjected to strong human impacts. The evidence presented suggests the potential circulation of bacteria between the environmental and clinical compartments, with gulls being a relevant vector of bacteria and resistance genes.ElsevierRepositório Científico do Instituto Nacional de SaúdeVarela, Ana RitaManageiro, VeraFerreira, EugéniaGuimarães, M. Augustada Costa, Paulo MartinsCaniça, ManuelaManaia, Célia M.2016-03-22T17:09:02Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/3718engJ Glob Antimicrob Resist. 2015 Dec.;3(4):286-9. doi.10.1016/j.jgar.2015.07.0082213-716510.1016/j.jgar.2015.07.008info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:57Zoai:repositorio.insa.pt:10400.18/3718Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:34.892122Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Molecular evidence of the close relatedness of clinical, gull and wastewater isolates of quinolone-resistant Escherichia coli
title Molecular evidence of the close relatedness of clinical, gull and wastewater isolates of quinolone-resistant Escherichia coli
spellingShingle Molecular evidence of the close relatedness of clinical, gull and wastewater isolates of quinolone-resistant Escherichia coli
Varela, Ana Rita
Resistência aos Antibióticos
Quinolone Resistance
Wildlife
Wastewater
Hospital
MLST
PFGE
Escherichia coli
title_short Molecular evidence of the close relatedness of clinical, gull and wastewater isolates of quinolone-resistant Escherichia coli
title_full Molecular evidence of the close relatedness of clinical, gull and wastewater isolates of quinolone-resistant Escherichia coli
title_fullStr Molecular evidence of the close relatedness of clinical, gull and wastewater isolates of quinolone-resistant Escherichia coli
title_full_unstemmed Molecular evidence of the close relatedness of clinical, gull and wastewater isolates of quinolone-resistant Escherichia coli
title_sort Molecular evidence of the close relatedness of clinical, gull and wastewater isolates of quinolone-resistant Escherichia coli
author Varela, Ana Rita
author_facet Varela, Ana Rita
Manageiro, Vera
Ferreira, Eugénia
Guimarães, M. Augusta
da Costa, Paulo Martins
Caniça, Manuela
Manaia, Célia M.
author_role author
author2 Manageiro, Vera
Ferreira, Eugénia
Guimarães, M. Augusta
da Costa, Paulo Martins
Caniça, Manuela
Manaia, Célia M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Varela, Ana Rita
Manageiro, Vera
Ferreira, Eugénia
Guimarães, M. Augusta
da Costa, Paulo Martins
Caniça, Manuela
Manaia, Célia M.
dc.subject.por.fl_str_mv Resistência aos Antibióticos
Quinolone Resistance
Wildlife
Wastewater
Hospital
MLST
PFGE
Escherichia coli
topic Resistência aos Antibióticos
Quinolone Resistance
Wildlife
Wastewater
Hospital
MLST
PFGE
Escherichia coli
description Short Communication
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2016-03-22T17:09:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/3718
url http://hdl.handle.net/10400.18/3718
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv J Glob Antimicrob Resist. 2015 Dec.;3(4):286-9. doi.10.1016/j.jgar.2015.07.008
2213-7165
10.1016/j.jgar.2015.07.008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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