Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration

Detalhes bibliográficos
Autor(a) principal: Calero-Cuenca, Francisco J.
Data de Publicação: 2021
Outros Autores: Osorio, Daniel S., Carvalho-Marques, Sofia, Sridhara, Sree Rama Chaitanya, Oliveira, Luis M., Jiao, Yue, Diaz, Jheimmy, Janota, Cátia, Cadot, Bruno, Gomes, Edgar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/49194
Resumo: © 2021 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0).
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spelling Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migrationCtdnep1Eps8L2LINC ComplexTAN linesActinCell migrationNuclear positioning© 2021 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0).Cells actively position their nuclei within the cytoplasm for multiple cellular and physiological functions.1-3 Consequently, nuclear mispositioning is usually associated with cell dysfunction and disease, from muscular disorders to cancer metastasis.4-7 Different cell types position their nuclei away from the leading edge during cell migration.8-11 In migrating fibroblasts, nuclear positioning is driven by an actin retrograde flow originated at the leading edge that drives dorsal actin cables away from the leading edge. The dorsal actin cables connect to the nuclear envelope by the linker of nucleoskeleton and cytoskeleton (LINC) complex on transmembrane actin-associated nuclear (TAN) lines.12-14 Dorsal actin cables are required for the formation of TAN lines. How dorsal actin cables are organized to promote TAN lines formation is unknown. Here, we report a role for Ctdnep1/Dullard, a nuclear envelope phosphatase,15-22 and the actin regulator Eps8L223-25 on nuclear positioning and cell migration. We demonstrate that Ctdnep1 and Eps8L2 directly interact, and this interaction is important for nuclear positioning and cell migration. We also show that Ctdnep1 and Eps8L2 are involved in the formation and thickness of dorsal actin cables required for TAN lines engagement during nuclear movement. We propose that Ctdnep1-Eps8L2 interaction regulates dorsal actin cables for nuclear movement during cell migration.This work was supported by the European Research Council (810207 and 617676); EMBO Installation (2758); Association pour la Recherche sur le Cancer; La Ligue Nacionale contre le Cancer; LISBOA-01-0145-FEDER-007391 project co-funded by FEDER, through POR Lisboa 2020 – Programa Operacional Regional de Lisboa, PORTUGAL 2020; and Fundação para a Ciência e a Tecnologia.ElsevierRepositório da Universidade de LisboaCalero-Cuenca, Francisco J.Osorio, Daniel S.Carvalho-Marques, SofiaSridhara, Sree Rama ChaitanyaOliveira, Luis M.Jiao, YueDiaz, JheimmyJanota, CátiaCadot, BrunoGomes, Edgar2021-07-28T14:44:13Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49194engCurr Biol. 2021 Apr 12;31(7):1521-1530.e80960-982210.1016/j.cub.2021.01.0071879-0445info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:52:41Zoai:repositorio.ul.pt:10451/49194Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:49.273298Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration
title Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration
spellingShingle Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration
Calero-Cuenca, Francisco J.
Ctdnep1
Eps8L2
LINC Complex
TAN lines
Actin
Cell migration
Nuclear positioning
title_short Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration
title_full Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration
title_fullStr Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration
title_full_unstemmed Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration
title_sort Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration
author Calero-Cuenca, Francisco J.
author_facet Calero-Cuenca, Francisco J.
Osorio, Daniel S.
Carvalho-Marques, Sofia
Sridhara, Sree Rama Chaitanya
Oliveira, Luis M.
Jiao, Yue
Diaz, Jheimmy
Janota, Cátia
Cadot, Bruno
Gomes, Edgar
author_role author
author2 Osorio, Daniel S.
Carvalho-Marques, Sofia
Sridhara, Sree Rama Chaitanya
Oliveira, Luis M.
Jiao, Yue
Diaz, Jheimmy
Janota, Cátia
Cadot, Bruno
Gomes, Edgar
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Calero-Cuenca, Francisco J.
Osorio, Daniel S.
Carvalho-Marques, Sofia
Sridhara, Sree Rama Chaitanya
Oliveira, Luis M.
Jiao, Yue
Diaz, Jheimmy
Janota, Cátia
Cadot, Bruno
Gomes, Edgar
dc.subject.por.fl_str_mv Ctdnep1
Eps8L2
LINC Complex
TAN lines
Actin
Cell migration
Nuclear positioning
topic Ctdnep1
Eps8L2
LINC Complex
TAN lines
Actin
Cell migration
Nuclear positioning
description © 2021 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0).
publishDate 2021
dc.date.none.fl_str_mv 2021-07-28T14:44:13Z
2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/49194
url http://hdl.handle.net/10451/49194
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Curr Biol. 2021 Apr 12;31(7):1521-1530.e8
0960-9822
10.1016/j.cub.2021.01.007
1879-0445
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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