MDA effects on morphophysiology and reproduction of Daphnia magna – preliminary data
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.48797/sl.2023.37 |
Resumo: | Background: Psychoactive substances and their metabolites are considered emergent contaminants, raising environmental concerns due to the continuous input into aquatic ecosystems [1]. MDA (3,4-methylenedioxyamphetamine) is a pharmacologically active substance that represents the major metabolite of 3,4-methylenedioxymethamphetamine (MDMA); MDA may be present in MDMA preparations or occur as an illegal psychoactive substance [2]. Considering the possible implementation of MDMA-assisted psychotherapy along with the increased recreational interest in MDA, the presence of MDA in aquatic ecosystems is expected to increase as well as its effects on non-target organisms, including invertebrates [1,3]. Since the ecotoxicological impact of MDA remains unknown, studying its toxic effects on an environmentally relevant organism is most important. Objective: This work aimed to assess the effects of possible MDA-induced toxicity on Daphnia magna through the evaluation of morphophysiological and reproductive endpoints. Methods: Neonates (<24 hours) were exposed to three concentrations of MDA (0.1, 1 and 10 mg/L) for 9 days. Exposures and control were performed with 5 replicates with 20 organisms each. On days 3 and 9, morphophysiological endpoints (body size, heart size and area, and heart rate) and reproductive endpoints (number of ovigerous daphnia and fertility) were determined. In parallel, a standard 21-d reproduction assay was conducted (MDA concentrations: 0, 0.10, 0.18, 0.32, 0.56, 1.00, 1.79 mg/L). Results: Morphophysiological endpoints increased with MDA concentration on the third and ninth day of exposure. An increase in the number of ovigerous daphnia and fecundity was found at 10 mg/L of MDA. In the 21-d assay, MDA caused a significant reduction in fecundity, body size and rate of increase from 0.18 mg/L upwards. Conclusions: Data are suggestive of some adverse influence of MDA on the analyzed endpoints. Nevertheless, other toxicity biomarkers should be evaluated to obtain insight on a mechanistic explanation for the observed effects. |
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MDA effects on morphophysiology and reproduction of Daphnia magna – preliminary dataPosterBackground: Psychoactive substances and their metabolites are considered emergent contaminants, raising environmental concerns due to the continuous input into aquatic ecosystems [1]. MDA (3,4-methylenedioxyamphetamine) is a pharmacologically active substance that represents the major metabolite of 3,4-methylenedioxymethamphetamine (MDMA); MDA may be present in MDMA preparations or occur as an illegal psychoactive substance [2]. Considering the possible implementation of MDMA-assisted psychotherapy along with the increased recreational interest in MDA, the presence of MDA in aquatic ecosystems is expected to increase as well as its effects on non-target organisms, including invertebrates [1,3]. Since the ecotoxicological impact of MDA remains unknown, studying its toxic effects on an environmentally relevant organism is most important. Objective: This work aimed to assess the effects of possible MDA-induced toxicity on Daphnia magna through the evaluation of morphophysiological and reproductive endpoints. Methods: Neonates (<24 hours) were exposed to three concentrations of MDA (0.1, 1 and 10 mg/L) for 9 days. Exposures and control were performed with 5 replicates with 20 organisms each. On days 3 and 9, morphophysiological endpoints (body size, heart size and area, and heart rate) and reproductive endpoints (number of ovigerous daphnia and fertility) were determined. In parallel, a standard 21-d reproduction assay was conducted (MDA concentrations: 0, 0.10, 0.18, 0.32, 0.56, 1.00, 1.79 mg/L). Results: Morphophysiological endpoints increased with MDA concentration on the third and ninth day of exposure. An increase in the number of ovigerous daphnia and fecundity was found at 10 mg/L of MDA. In the 21-d assay, MDA caused a significant reduction in fecundity, body size and rate of increase from 0.18 mg/L upwards. Conclusions: Data are suggestive of some adverse influence of MDA on the analyzed endpoints. Nevertheless, other toxicity biomarkers should be evaluated to obtain insight on a mechanistic explanation for the observed effects.IUCS-CESPU Publishing2023-04-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.48797/sl.2023.37https://doi.org/10.48797/sl.2023.37Scientific Letters; Vol. 1 No. Sup 1 (2023)2795-5117reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttps://publicacoes.cespu.pt/index.php/sl/article/view/37https://publicacoes.cespu.pt/index.php/sl/article/view/37/52Copyright (c) 2023 A. M. Morão, A. R. Carvalho, C. Couto, J. S. Carrola, P. Costa, B. Castro, M. E. Tiritan, C. Ribeiroinfo:eu-repo/semantics/openAccessMorão, A. M.Carvalho, A. R.Couto, C.Carrola, J. S.Costa, P.Castro, B.Tiritan, M. E.Ribeiro, C.2023-04-29T08:45:57Zoai:publicacoes.cespu.pt:article/37Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:50:21.176087Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
MDA effects on morphophysiology and reproduction of Daphnia magna – preliminary data |
title |
MDA effects on morphophysiology and reproduction of Daphnia magna – preliminary data |
spellingShingle |
MDA effects on morphophysiology and reproduction of Daphnia magna – preliminary data Morão, A. M. Poster |
title_short |
MDA effects on morphophysiology and reproduction of Daphnia magna – preliminary data |
title_full |
MDA effects on morphophysiology and reproduction of Daphnia magna – preliminary data |
title_fullStr |
MDA effects on morphophysiology and reproduction of Daphnia magna – preliminary data |
title_full_unstemmed |
MDA effects on morphophysiology and reproduction of Daphnia magna – preliminary data |
title_sort |
MDA effects on morphophysiology and reproduction of Daphnia magna – preliminary data |
author |
Morão, A. M. |
author_facet |
Morão, A. M. Carvalho, A. R. Couto, C. Carrola, J. S. Costa, P. Castro, B. Tiritan, M. E. Ribeiro, C. |
author_role |
author |
author2 |
Carvalho, A. R. Couto, C. Carrola, J. S. Costa, P. Castro, B. Tiritan, M. E. Ribeiro, C. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Morão, A. M. Carvalho, A. R. Couto, C. Carrola, J. S. Costa, P. Castro, B. Tiritan, M. E. Ribeiro, C. |
dc.subject.por.fl_str_mv |
Poster |
topic |
Poster |
description |
Background: Psychoactive substances and their metabolites are considered emergent contaminants, raising environmental concerns due to the continuous input into aquatic ecosystems [1]. MDA (3,4-methylenedioxyamphetamine) is a pharmacologically active substance that represents the major metabolite of 3,4-methylenedioxymethamphetamine (MDMA); MDA may be present in MDMA preparations or occur as an illegal psychoactive substance [2]. Considering the possible implementation of MDMA-assisted psychotherapy along with the increased recreational interest in MDA, the presence of MDA in aquatic ecosystems is expected to increase as well as its effects on non-target organisms, including invertebrates [1,3]. Since the ecotoxicological impact of MDA remains unknown, studying its toxic effects on an environmentally relevant organism is most important. Objective: This work aimed to assess the effects of possible MDA-induced toxicity on Daphnia magna through the evaluation of morphophysiological and reproductive endpoints. Methods: Neonates (<24 hours) were exposed to three concentrations of MDA (0.1, 1 and 10 mg/L) for 9 days. Exposures and control were performed with 5 replicates with 20 organisms each. On days 3 and 9, morphophysiological endpoints (body size, heart size and area, and heart rate) and reproductive endpoints (number of ovigerous daphnia and fertility) were determined. In parallel, a standard 21-d reproduction assay was conducted (MDA concentrations: 0, 0.10, 0.18, 0.32, 0.56, 1.00, 1.79 mg/L). Results: Morphophysiological endpoints increased with MDA concentration on the third and ninth day of exposure. An increase in the number of ovigerous daphnia and fecundity was found at 10 mg/L of MDA. In the 21-d assay, MDA caused a significant reduction in fecundity, body size and rate of increase from 0.18 mg/L upwards. Conclusions: Data are suggestive of some adverse influence of MDA on the analyzed endpoints. Nevertheless, other toxicity biomarkers should be evaluated to obtain insight on a mechanistic explanation for the observed effects. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-21 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.48797/sl.2023.37 https://doi.org/10.48797/sl.2023.37 |
url |
https://doi.org/10.48797/sl.2023.37 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://publicacoes.cespu.pt/index.php/sl/article/view/37 https://publicacoes.cespu.pt/index.php/sl/article/view/37/52 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
IUCS-CESPU Publishing |
publisher.none.fl_str_mv |
IUCS-CESPU Publishing |
dc.source.none.fl_str_mv |
Scientific Letters; Vol. 1 No. Sup 1 (2023) 2795-5117 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131582961811456 |