Melanin transferred to keratinocytes resides in non-degradative endocytic compartments
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/149314 |
Resumo: | Funding: We thank the staff from the Unit of Imaging and Cytometry of the Instituto Gulbenkian de Ciência for assistance in microscopy and flow cytometry protocol adjustments, the CEDOC Microscopy Facility for assistance in image acquisition and analysis, Dot Bennett for the kind gift of cell lines, and Alistair Hume for the kind gift of anti-Rab5 antibody. This project was supported by Fundação para a Ciência e a Tecnologia (FCT), Portugal (PTDC/BIA-BCM/ 111735/2009, EXPL/BEX-BCM/0379/2013), MSC and HM were supported by FCT PhD studentships (SFRH/BD/65381/2009 and PD/BD/114118/2015, respectively), FJCP was supported by an FCT postdoctoral fellowship (SFRH/ BPD/70337/2010), and DCB was supported by the FCT Investigator Program (IF/00501/2014/CP1252/CT0001). |
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Melanin transferred to keratinocytes resides in non-degradative endocytic compartmentsLAMPlysosomal-associated membrane proteinPARPBSphosphate buffered salineprotease-activated receptorsiRNAsmall interfering RNAFunding: We thank the staff from the Unit of Imaging and Cytometry of the Instituto Gulbenkian de Ciência for assistance in microscopy and flow cytometry protocol adjustments, the CEDOC Microscopy Facility for assistance in image acquisition and analysis, Dot Bennett for the kind gift of cell lines, and Alistair Hume for the kind gift of anti-Rab5 antibody. This project was supported by Fundação para a Ciência e a Tecnologia (FCT), Portugal (PTDC/BIA-BCM/ 111735/2009, EXPL/BEX-BCM/0379/2013), MSC and HM were supported by FCT PhD studentships (SFRH/BD/65381/2009 and PD/BD/114118/2015, respectively), FJCP was supported by an FCT postdoctoral fellowship (SFRH/ BPD/70337/2010), and DCB was supported by the FCT Investigator Program (IF/00501/2014/CP1252/CT0001).Melanin transfer from melanocytes to keratinocytes and subsequent accumulation in the supra-nuclear region is a critical process in skin pigmentation and protection against ultraviolet radiation. We have previously proposed that the main mode of transfer between melanocytes and keratinocytes is through exo/endocytosis of the melanosome core, termed melanocore. In this study, we developed an in vitro uptake assay using melanocores secreted by melanocytes. We show that the uptake of melanocores, but not melanosomes, by keratinocytes is Protease-activated receptor (PAR)-2-dependent. Furthermore, we found that the silencing of the early endocytic regulator Rab5b, but not the late endocytic regulators Rab7a or Rab9a, significantly impairs melanocore uptake by keratinocytes. Following uptake, we observed that melanin accumulates in compartments that are positive for both early and late endocytic markers. We found that melanin does not localize to either highly degradative or acidic organelles, as assessed by LysoTracker and DQ-BSA staining, despite the abundance of these types of organelles within keratinocytes. Therefore, we propose that melanocore uptake leads to storage of melanin within keratinocytes in hybrid endocytic compartments that are not highly acidic or degradative. By avoiding lysosomal degradation, these specialized endosomes may allow melanin to persist within keratinocytes for long periods.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNCorreia, Maria SMoreiras, HugoPereira, Francisco J CNeto, Matilde VFestas, Tiago CTarafder, Abul KRamalho, José SSeabra, Miguel CBarral, Duarte C2023-02-16T22:13:43Z2018-032018-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/10362/149314eng0022-202XPURE: 3278434https://doi.org/10.1016/j.jid.2017.09.042info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:31:15Zoai:run.unl.pt:10362/149314Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:53:42.936966Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Melanin transferred to keratinocytes resides in non-degradative endocytic compartments |
| title |
Melanin transferred to keratinocytes resides in non-degradative endocytic compartments |
| spellingShingle |
Melanin transferred to keratinocytes resides in non-degradative endocytic compartments Correia, Maria S LAMP lysosomal-associated membrane protein PAR PBS phosphate buffered saline protease-activated receptor siRNA small interfering RNA |
| title_short |
Melanin transferred to keratinocytes resides in non-degradative endocytic compartments |
| title_full |
Melanin transferred to keratinocytes resides in non-degradative endocytic compartments |
| title_fullStr |
Melanin transferred to keratinocytes resides in non-degradative endocytic compartments |
| title_full_unstemmed |
Melanin transferred to keratinocytes resides in non-degradative endocytic compartments |
| title_sort |
Melanin transferred to keratinocytes resides in non-degradative endocytic compartments |
| author |
Correia, Maria S |
| author_facet |
Correia, Maria S Moreiras, Hugo Pereira, Francisco J C Neto, Matilde V Festas, Tiago C Tarafder, Abul K Ramalho, José S Seabra, Miguel C Barral, Duarte C |
| author_role |
author |
| author2 |
Moreiras, Hugo Pereira, Francisco J C Neto, Matilde V Festas, Tiago C Tarafder, Abul K Ramalho, José S Seabra, Miguel C Barral, Duarte C |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centro de Estudos de Doenças Crónicas (CEDOC) RUN |
| dc.contributor.author.fl_str_mv |
Correia, Maria S Moreiras, Hugo Pereira, Francisco J C Neto, Matilde V Festas, Tiago C Tarafder, Abul K Ramalho, José S Seabra, Miguel C Barral, Duarte C |
| dc.subject.por.fl_str_mv |
LAMP lysosomal-associated membrane protein PAR PBS phosphate buffered saline protease-activated receptor siRNA small interfering RNA |
| topic |
LAMP lysosomal-associated membrane protein PAR PBS phosphate buffered saline protease-activated receptor siRNA small interfering RNA |
| description |
Funding: We thank the staff from the Unit of Imaging and Cytometry of the Instituto Gulbenkian de Ciência for assistance in microscopy and flow cytometry protocol adjustments, the CEDOC Microscopy Facility for assistance in image acquisition and analysis, Dot Bennett for the kind gift of cell lines, and Alistair Hume for the kind gift of anti-Rab5 antibody. This project was supported by Fundação para a Ciência e a Tecnologia (FCT), Portugal (PTDC/BIA-BCM/ 111735/2009, EXPL/BEX-BCM/0379/2013), MSC and HM were supported by FCT PhD studentships (SFRH/BD/65381/2009 and PD/BD/114118/2015, respectively), FJCP was supported by an FCT postdoctoral fellowship (SFRH/ BPD/70337/2010), and DCB was supported by the FCT Investigator Program (IF/00501/2014/CP1252/CT0001). |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-03 2018-03-01T00:00:00Z 2023-02-16T22:13:43Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
| format |
article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/149314 |
| url |
http://hdl.handle.net/10362/149314 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
0022-202X PURE: 3278434 https://doi.org/10.1016/j.jid.2017.09.042 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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10 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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