A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium

Detalhes bibliográficos
Autor(a) principal: Slavic, Ksenija
Data de Publicação: 2016
Outros Autores: Krishna, Sanjeev, Lahree, Aparajita, Bouyer, Guillaume, Hanson, Kirsten K., Vera, Iset, Pittman, Jon K., Staines, Henry M., Mota, Maria M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/51630
Resumo: © Copyright © 2016, The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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spelling A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium© Copyright © 2016, The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/Iron is an essential micronutrient but is also highly toxic. In yeast and plant cells, a key detoxifying mechanism involves iron sequestration into intracellular storage compartments, mediated by members of the vacuolar iron-transporter (VIT) family of proteins. Here we study the VIT homologue from the malaria parasites Plasmodium falciparum (PfVIT) and Plasmodium berghei (PbVIT). PfVIT-mediated iron transport in a yeast heterologous expression system is saturable (Km ∼ 14.7 μM), and selective for Fe(2+) over other divalent cations. PbVIT-deficient P. berghei lines (Pbvit(-)) show a reduction in parasite load in both liver and blood stages of infection in mice. Moreover, Pbvit(-) parasites have higher levels of labile iron in blood stages and are more sensitive to increased iron levels in liver stages, when compared with wild-type parasites. Our data are consistent with Plasmodium VITs playing a major role in iron detoxification and, thus, normal development of malaria parasites in their mammalian host.This work was supported by grants from the European Research Council ERC-2012-StG_311502 to M.M.M., Fundação para a Ciência e Tecnologia EXPL/BIM-MET/0753/2013 and The European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement n°. 304948—NANOMAL to S.K. and H.M.S. K.S. was supported by an EMBO long-term fellowship (EMBO ALTF 1584-2011).Springer NatureRepositório da Universidade de LisboaSlavic, KsenijaKrishna, SanjeevLahree, AparajitaBouyer, GuillaumeHanson, Kirsten K.Vera, IsetPittman, Jon K.Staines, Henry M.Mota, Maria M.2022-03-07T15:37:08Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51630engNat Commun. 2016 Jan 20;7:1040310.1038/ncomms104032041-1723info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:56:27Zoai:repositorio.ul.pt:10451/51630Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:53.459836Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium
title A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium
spellingShingle A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium
Slavic, Ksenija
title_short A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium
title_full A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium
title_fullStr A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium
title_full_unstemmed A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium
title_sort A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium
author Slavic, Ksenija
author_facet Slavic, Ksenija
Krishna, Sanjeev
Lahree, Aparajita
Bouyer, Guillaume
Hanson, Kirsten K.
Vera, Iset
Pittman, Jon K.
Staines, Henry M.
Mota, Maria M.
author_role author
author2 Krishna, Sanjeev
Lahree, Aparajita
Bouyer, Guillaume
Hanson, Kirsten K.
Vera, Iset
Pittman, Jon K.
Staines, Henry M.
Mota, Maria M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Slavic, Ksenija
Krishna, Sanjeev
Lahree, Aparajita
Bouyer, Guillaume
Hanson, Kirsten K.
Vera, Iset
Pittman, Jon K.
Staines, Henry M.
Mota, Maria M.
description © Copyright © 2016, The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
2022-03-07T15:37:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/51630
url http://hdl.handle.net/10451/51630
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nat Commun. 2016 Jan 20;7:10403
10.1038/ncomms10403
2041-1723
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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