Evaluation of dentinogenesis inducer biomaterials: an in vivo study
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/106238 https://doi.org/10.1590/1678-7757-2019-0023 |
Resumo: | When exposure of the pulp to external environment occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain pulp tissue vitality and function. These clinical situations require the use of materials that induce dentin repair and, subsequently, formation of a mineralized tissue. Objective: This work aims to assess the effect of tricalcium silicate cements and mineral trioxide aggregate cements, including repairing dentin formation and inflammatory reactions over time after pulp exposure in Wistar rats. Methodology: These two biomaterials were compared with positive control groups (open cavity with pulp tissue exposure) and negative control groups (no intervention). The evaluations were performed in three stages; three, seven and twenty-one days, and consisted of an imaging (nuclear medicine) and histological evaluation (H&E staining, immunohistochemistry and Alizarin Red S). Results: The therapeutic effect of these biomaterials was confirmed. Nuclear medicine evaluation demonstrated that the uptake of 99mTc- Hydroxymethylene diphosphonate (HMDP) showed no significant differences between the different experimental groups and the control, revealing the nonoccurrence of differences in the phosphocalcium metabolism. The histological study demonstrated that in mineral trioxide aggregate therapies, the presence of moderate inflammatory infiltration was found after three days, decreasing during follow-ups. The formation of mineralized tissue was only verified at 21 days of follow-up. The tricalcium silicate therapies demonstrated the presence of a slight inflammatory infiltration on the third day, increasing throughout the follow-up. The formation of mineralized tissue was observed in the seventh follow-up day, increasing over time. Conclusions: The mineral trioxide aggregate (WhiteProRoot®MTA) and tricalcium silicate (Biodentine™) present slight and reversible inflammatory signs in the pulp tissue, with the formation of mineralized tissue. However, the exacerbated induction of mineralized tissue formation with the tricalcium silicate biomaterial may lead to the formation of pulp calcifications |
id |
RCAP_c718777a01515186fbd84822dbe3c6bb |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/106238 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Evaluation of dentinogenesis inducer biomaterials: an in vivo studyBiomaterialsDentinogenesisDental pulpOdontoblastPulp cappingAluminum CompoundsAnimalsBiocompatible MaterialsCalcium CompoundsDental PulpDental Pulp CappingDental Pulp ExposureDentinDentinogenesisDrug CombinationsExtracellular Matrix ProteinsImmunohistochemistryMaleMolecular ImagingOdontoblastsOxidesPhosphoproteinsPulp Capping and Pulpectomy AgentsPulpitisRandom AllocationRats, WistarReproducibility of ResultsSialoglycoproteinsSilicatesTime FactorsWhen exposure of the pulp to external environment occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain pulp tissue vitality and function. These clinical situations require the use of materials that induce dentin repair and, subsequently, formation of a mineralized tissue. Objective: This work aims to assess the effect of tricalcium silicate cements and mineral trioxide aggregate cements, including repairing dentin formation and inflammatory reactions over time after pulp exposure in Wistar rats. Methodology: These two biomaterials were compared with positive control groups (open cavity with pulp tissue exposure) and negative control groups (no intervention). The evaluations were performed in three stages; three, seven and twenty-one days, and consisted of an imaging (nuclear medicine) and histological evaluation (H&E staining, immunohistochemistry and Alizarin Red S). Results: The therapeutic effect of these biomaterials was confirmed. Nuclear medicine evaluation demonstrated that the uptake of 99mTc- Hydroxymethylene diphosphonate (HMDP) showed no significant differences between the different experimental groups and the control, revealing the nonoccurrence of differences in the phosphocalcium metabolism. The histological study demonstrated that in mineral trioxide aggregate therapies, the presence of moderate inflammatory infiltration was found after three days, decreasing during follow-ups. The formation of mineralized tissue was only verified at 21 days of follow-up. The tricalcium silicate therapies demonstrated the presence of a slight inflammatory infiltration on the third day, increasing throughout the follow-up. The formation of mineralized tissue was observed in the seventh follow-up day, increasing over time. Conclusions: The mineral trioxide aggregate (WhiteProRoot®MTA) and tricalcium silicate (Biodentine™) present slight and reversible inflammatory signs in the pulp tissue, with the formation of mineralized tissue. However, the exacerbated induction of mineralized tissue formation with the tricalcium silicate biomaterial may lead to the formation of pulp calcificationsFaculdade de Odontologia de Bauru da Universidade de Sao Paulo2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/106238http://hdl.handle.net/10316/106238https://doi.org/10.1590/1678-7757-2019-0023eng1678-77651678-7757Paula, Anabela B.Laranjo, MafaldaMarto, Carlos MiguelPaulo, Siri Folques Vicente deAbrantes, Ana M.Fernandes, BrunoCasalta-Lopes, JoãoFerreira, Manuel MarquesBotelho, Maria FilomenaCarrilho, Euniceinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-27T20:34:03Zoai:estudogeral.uc.pt:10316/106238Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:22:43.509184Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluation of dentinogenesis inducer biomaterials: an in vivo study |
title |
Evaluation of dentinogenesis inducer biomaterials: an in vivo study |
spellingShingle |
Evaluation of dentinogenesis inducer biomaterials: an in vivo study Paula, Anabela B. Biomaterials Dentinogenesis Dental pulp Odontoblast Pulp capping Aluminum Compounds Animals Biocompatible Materials Calcium Compounds Dental Pulp Dental Pulp Capping Dental Pulp Exposure Dentin Dentinogenesis Drug Combinations Extracellular Matrix Proteins Immunohistochemistry Male Molecular Imaging Odontoblasts Oxides Phosphoproteins Pulp Capping and Pulpectomy Agents Pulpitis Random Allocation Rats, Wistar Reproducibility of Results Sialoglycoproteins Silicates Time Factors |
title_short |
Evaluation of dentinogenesis inducer biomaterials: an in vivo study |
title_full |
Evaluation of dentinogenesis inducer biomaterials: an in vivo study |
title_fullStr |
Evaluation of dentinogenesis inducer biomaterials: an in vivo study |
title_full_unstemmed |
Evaluation of dentinogenesis inducer biomaterials: an in vivo study |
title_sort |
Evaluation of dentinogenesis inducer biomaterials: an in vivo study |
author |
Paula, Anabela B. |
author_facet |
Paula, Anabela B. Laranjo, Mafalda Marto, Carlos Miguel Paulo, Siri Folques Vicente de Abrantes, Ana M. Fernandes, Bruno Casalta-Lopes, João Ferreira, Manuel Marques Botelho, Maria Filomena Carrilho, Eunice |
author_role |
author |
author2 |
Laranjo, Mafalda Marto, Carlos Miguel Paulo, Siri Folques Vicente de Abrantes, Ana M. Fernandes, Bruno Casalta-Lopes, João Ferreira, Manuel Marques Botelho, Maria Filomena Carrilho, Eunice |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Paula, Anabela B. Laranjo, Mafalda Marto, Carlos Miguel Paulo, Siri Folques Vicente de Abrantes, Ana M. Fernandes, Bruno Casalta-Lopes, João Ferreira, Manuel Marques Botelho, Maria Filomena Carrilho, Eunice |
dc.subject.por.fl_str_mv |
Biomaterials Dentinogenesis Dental pulp Odontoblast Pulp capping Aluminum Compounds Animals Biocompatible Materials Calcium Compounds Dental Pulp Dental Pulp Capping Dental Pulp Exposure Dentin Dentinogenesis Drug Combinations Extracellular Matrix Proteins Immunohistochemistry Male Molecular Imaging Odontoblasts Oxides Phosphoproteins Pulp Capping and Pulpectomy Agents Pulpitis Random Allocation Rats, Wistar Reproducibility of Results Sialoglycoproteins Silicates Time Factors |
topic |
Biomaterials Dentinogenesis Dental pulp Odontoblast Pulp capping Aluminum Compounds Animals Biocompatible Materials Calcium Compounds Dental Pulp Dental Pulp Capping Dental Pulp Exposure Dentin Dentinogenesis Drug Combinations Extracellular Matrix Proteins Immunohistochemistry Male Molecular Imaging Odontoblasts Oxides Phosphoproteins Pulp Capping and Pulpectomy Agents Pulpitis Random Allocation Rats, Wistar Reproducibility of Results Sialoglycoproteins Silicates Time Factors |
description |
When exposure of the pulp to external environment occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain pulp tissue vitality and function. These clinical situations require the use of materials that induce dentin repair and, subsequently, formation of a mineralized tissue. Objective: This work aims to assess the effect of tricalcium silicate cements and mineral trioxide aggregate cements, including repairing dentin formation and inflammatory reactions over time after pulp exposure in Wistar rats. Methodology: These two biomaterials were compared with positive control groups (open cavity with pulp tissue exposure) and negative control groups (no intervention). The evaluations were performed in three stages; three, seven and twenty-one days, and consisted of an imaging (nuclear medicine) and histological evaluation (H&E staining, immunohistochemistry and Alizarin Red S). Results: The therapeutic effect of these biomaterials was confirmed. Nuclear medicine evaluation demonstrated that the uptake of 99mTc- Hydroxymethylene diphosphonate (HMDP) showed no significant differences between the different experimental groups and the control, revealing the nonoccurrence of differences in the phosphocalcium metabolism. The histological study demonstrated that in mineral trioxide aggregate therapies, the presence of moderate inflammatory infiltration was found after three days, decreasing during follow-ups. The formation of mineralized tissue was only verified at 21 days of follow-up. The tricalcium silicate therapies demonstrated the presence of a slight inflammatory infiltration on the third day, increasing throughout the follow-up. The formation of mineralized tissue was observed in the seventh follow-up day, increasing over time. Conclusions: The mineral trioxide aggregate (WhiteProRoot®MTA) and tricalcium silicate (Biodentine™) present slight and reversible inflammatory signs in the pulp tissue, with the formation of mineralized tissue. However, the exacerbated induction of mineralized tissue formation with the tricalcium silicate biomaterial may lead to the formation of pulp calcifications |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/106238 http://hdl.handle.net/10316/106238 https://doi.org/10.1590/1678-7757-2019-0023 |
url |
http://hdl.handle.net/10316/106238 https://doi.org/10.1590/1678-7757-2019-0023 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1678-7765 1678-7757 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Faculdade de Odontologia de Bauru da Universidade de Sao Paulo |
publisher.none.fl_str_mv |
Faculdade de Odontologia de Bauru da Universidade de Sao Paulo |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799134115665018880 |