Phototoxic Potential of Different DNA Intercalators for Skin Cancer Therapy
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/154933 |
Resumo: | This research was funded by Fundação para a Ciência e a Tecnologia (FCT-MCTES), Radiation Biology and the Biophysics Doctoral Training Programme (RaBBiT, PD/00193/2012); the scholarship grant number PD/BD/142829/2018, to T.P.P. from the RaBBiT Doctoral Training Programme. Publisher Copyright: © 2023 by the authors. |
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Phototoxic Potential of Different DNA Intercalators for Skin Cancer TherapyIn Vitro Screeningphotodynamic therapyphotosensitizerreactive oxygen speciesskin cancerCatalysisMolecular BiologySpectroscopyComputer Science ApplicationsPhysical and Theoretical ChemistryOrganic ChemistryInorganic ChemistrySDG 3 - Good Health and Well-beingThis research was funded by Fundação para a Ciência e a Tecnologia (FCT-MCTES), Radiation Biology and the Biophysics Doctoral Training Programme (RaBBiT, PD/00193/2012); the scholarship grant number PD/BD/142829/2018, to T.P.P. from the RaBBiT Doctoral Training Programme. Publisher Copyright: © 2023 by the authors.Photodynamic therapy is a minimally invasive procedure used in the treatment of several diseases, including some types of cancer. It is based on photosensitizer molecules, which, in the presence of oxygen and light, lead to the formation of reactive oxygen species (ROS) and consequent cell death. The selection of the photosensitizer molecule is important for the therapy efficiency; therefore, many molecules such as dyes, natural products and metallic complexes have been investigated regarding their photosensitizing potential. In this work, the phototoxic potential of the DNA-intercalating molecules—the dyes methylene blue (MB), acridine orange (AO) and gentian violet (GV); the natural products curcumin (CUR), quercetin (QT) and epigallocatechin gallate (EGCG); and the chelating compounds neocuproine (NEO), 1,10-phenanthroline (PHE) and 2,2′-bipyridyl (BIPY)—were analyzed. The cytotoxicity of these chemicals was tested in vitro in non-cancer keratinocytes (HaCaT) and squamous cell carcinoma (MET1) cell lines. A phototoxicity assay and the detection of intracellular ROS were performed in MET1 cells. Results revealed that the IC50 values of the dyes and curcumin in MET1 cells were lower than 30 µM, while the values for the natural products QT and EGCG and the chelating agents BIPY and PHE were higher than 100 µM. The IC50 of MB and AO was greatly affected by irradiation when submitted to 640 nm and 457 nm light sources, respectively. ROS detection was more evident for cells treated with AO at low concentrations. In studies with the melanoma cell line WM983b, cells were more resistant to MB and AO and presented slightly higher IC50 values, in line with the results of the phototoxicity assays. This study reveals that many molecules can act as photosensitizers, but the effect depends on the cell line and the concentration of the chemical. Finally, significant photosensitizing activity of acridine orange at low concentrations and moderate light doses was demonstrated.DF – Departamento de FísicaCeFITec – Centro de Física e Investigação TecnológicaLIBPhys-UNLCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)RUNPivetta, Thais P.Vieira, TâniaSilva, Jorge C.Ribeiro, Paulo A.Raposo, Maria2023-07-06T22:16:45Z2023-03-152023-03-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://hdl.handle.net/10362/154933eng1661-6596PURE: 65372598https://doi.org/10.3390/ijms24065602info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:12:41Zoai:run.unl.pt:10362/154933Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:12:41Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Phototoxic Potential of Different DNA Intercalators for Skin Cancer Therapy In Vitro Screening |
| title |
Phototoxic Potential of Different DNA Intercalators for Skin Cancer Therapy |
| spellingShingle |
Phototoxic Potential of Different DNA Intercalators for Skin Cancer Therapy Pivetta, Thais P. photodynamic therapy photosensitizer reactive oxygen species skin cancer Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| title_short |
Phototoxic Potential of Different DNA Intercalators for Skin Cancer Therapy |
| title_full |
Phototoxic Potential of Different DNA Intercalators for Skin Cancer Therapy |
| title_fullStr |
Phototoxic Potential of Different DNA Intercalators for Skin Cancer Therapy |
| title_full_unstemmed |
Phototoxic Potential of Different DNA Intercalators for Skin Cancer Therapy |
| title_sort |
Phototoxic Potential of Different DNA Intercalators for Skin Cancer Therapy |
| author |
Pivetta, Thais P. |
| author_facet |
Pivetta, Thais P. Vieira, Tânia Silva, Jorge C. Ribeiro, Paulo A. Raposo, Maria |
| author_role |
author |
| author2 |
Vieira, Tânia Silva, Jorge C. Ribeiro, Paulo A. Raposo, Maria |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
DF – Departamento de Física CeFITec – Centro de Física e Investigação Tecnológica LIBPhys-UNL CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) RUN |
| dc.contributor.author.fl_str_mv |
Pivetta, Thais P. Vieira, Tânia Silva, Jorge C. Ribeiro, Paulo A. Raposo, Maria |
| dc.subject.por.fl_str_mv |
photodynamic therapy photosensitizer reactive oxygen species skin cancer Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| topic |
photodynamic therapy photosensitizer reactive oxygen species skin cancer Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| description |
This research was funded by Fundação para a Ciência e a Tecnologia (FCT-MCTES), Radiation Biology and the Biophysics Doctoral Training Programme (RaBBiT, PD/00193/2012); the scholarship grant number PD/BD/142829/2018, to T.P.P. from the RaBBiT Doctoral Training Programme. Publisher Copyright: © 2023 by the authors. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-07-06T22:16:45Z 2023-03-15 2023-03-15T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/154933 |
| url |
http://hdl.handle.net/10362/154933 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1661-6596 PURE: 65372598 https://doi.org/10.3390/ijms24065602 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
16 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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mluisa.alvim@gmail.com |
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1817545942868426752 |