A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach
| Autor(a) principal: | |
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| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/52286 |
Resumo: | Supplementary Materials - The following are available online at https://www.mdpi.com/1422-0067/21/24/9681/s1, Figure S1: GNPs’ size distribution by intensity (%) obtained by DLS. |
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A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal ApproachBreast cancerExperimental modelDMBALaser photothermal therapyGold nanoparticlesEpigenetic alterationsSupplementary Materials - The following are available online at https://www.mdpi.com/1422-0067/21/24/9681/s1, Figure S1: GNPs’ size distribution by intensity (%) obtained by DLS.Breast cancer is one of the most frequently diagnosed malignancies and common causes of cancer death in women. Recent studies suggest that environmental exposures to certain chemicals, such as 7,12-Dimethylbenzanthracene (DMBA), a chemical present in tobacco, may increase the risk of developing breast cancer later in life. The first-line treatments for breast cancer (surgery, chemotherapy or a combination of both) are generally invasive and frequently associated with severe side effects and high comorbidity. Consequently, novel approaches are strongly required to find more natural-like experimental models that better reflect the tumors’ etiology, physiopathology and response to treatments, as well as to find more targeted, efficient and minimally invasive treatments. This study proposes the development and an in deep biological characterization of an experimental model using DMBA-tumor-induction in Sprague-Dawley female rats. Moreover, a photothermal therapy approach using a near-infrared laser coupled with gold nanoparticles was preliminarily assessed. The gold nanoparticles were functionalized with Epidermal Growth Factor, and their physicochemical properties and in vitro effects were characterized. DMBA proved to be a very good and selective inductor of breast cancer, with 100% incidence and inducing an average of 4.7 tumors per animal. Epigenetic analysis showed that tumors classified with worst prognosis were hypomethylated. The tumor-induced rats were then subjected to a preliminary treatment using functionalized gold nanoparticles and its activation by laser (650–900 nm). The treatment outcomes presented very promising alterations in terms of tumor histology, confirming the presence of necrosis in most of the cases. Although this study revealed encouraging results as a breast cancer therapy, it is important to define tumor eligibility and specific efficiency criteria to further assess its application in breast cancer treatment on other species.The APC was funded by Faculty of Pharmacy, University of Coimbra and Coimbra Chemistry Centre, Department of Chemistry, University of Coimbra. This work was also supported by Fundação para a Ciência e Tecnologia (FCT), Portugal under the project’s references UIDB/00645/2020 and UID/DTP/04138/2019. TFG was supported by FCT, Portugal under the reference SFRH/BD/147306/2019. Thanks to FCT/MCTES for the financial support to CESAM (UIDP/50017/2020+UIDB/50017/2020), through national funds.MDPIRepositório da Universidade de LisboaCosta, EduardoFerreira-Gonçalves, TâniaCardoso, MiguelCoelho, João M. P.Gaspar, Maria ManuelaFaísca, PedroAscensão, LiaCabrita, António S.Reis, Catarina PintoFigueiredo, Isabel V.2022-04-11T17:25:12Z2020-12-182022-02-21T09:40:17Z2020-12-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/52286engCosta E, Ferreira-Gonçalves T, Cardoso M, Coelho JMP, Gaspar MM, Faísca P, et al. A step forward in breast cancer research: from a natural-like experimental model to a preliminary photothermal approach. International Journal of Molecular Sciences [Internet]. 2020;21(24):9681. Disponível em: https://www.mdpi.com/1422-0067/21/24/96811422-0067cv-prod-2118247https://doi.org/10.3390/ijms212496812-s2.0-85098071015info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:56:06Zoai:repositorio.ul.pt:10451/52286Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:42.530901Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach |
| title |
A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach |
| spellingShingle |
A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach Costa, Eduardo Breast cancer Experimental model DMBA Laser photothermal therapy Gold nanoparticles Epigenetic alterations |
| title_short |
A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach |
| title_full |
A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach |
| title_fullStr |
A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach |
| title_full_unstemmed |
A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach |
| title_sort |
A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach |
| author |
Costa, Eduardo |
| author_facet |
Costa, Eduardo Ferreira-Gonçalves, Tânia Cardoso, Miguel Coelho, João M. P. Gaspar, Maria Manuela Faísca, Pedro Ascensão, Lia Cabrita, António S. Reis, Catarina Pinto Figueiredo, Isabel V. |
| author_role |
author |
| author2 |
Ferreira-Gonçalves, Tânia Cardoso, Miguel Coelho, João M. P. Gaspar, Maria Manuela Faísca, Pedro Ascensão, Lia Cabrita, António S. Reis, Catarina Pinto Figueiredo, Isabel V. |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Costa, Eduardo Ferreira-Gonçalves, Tânia Cardoso, Miguel Coelho, João M. P. Gaspar, Maria Manuela Faísca, Pedro Ascensão, Lia Cabrita, António S. Reis, Catarina Pinto Figueiredo, Isabel V. |
| dc.subject.por.fl_str_mv |
Breast cancer Experimental model DMBA Laser photothermal therapy Gold nanoparticles Epigenetic alterations |
| topic |
Breast cancer Experimental model DMBA Laser photothermal therapy Gold nanoparticles Epigenetic alterations |
| description |
Supplementary Materials - The following are available online at https://www.mdpi.com/1422-0067/21/24/9681/s1, Figure S1: GNPs’ size distribution by intensity (%) obtained by DLS. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-12-18 2020-12-18T00:00:00Z 2022-04-11T17:25:12Z 2022-02-21T09:40:17Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10451/52286 |
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http://hdl.handle.net/10451/52286 |
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eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Costa E, Ferreira-Gonçalves T, Cardoso M, Coelho JMP, Gaspar MM, Faísca P, et al. A step forward in breast cancer research: from a natural-like experimental model to a preliminary photothermal approach. International Journal of Molecular Sciences [Internet]. 2020;21(24):9681. Disponível em: https://www.mdpi.com/1422-0067/21/24/9681 1422-0067 cv-prod-2118247 https://doi.org/10.3390/ijms21249681 2-s2.0-85098071015 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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