The inhibition of Cathepsin G on endometrial explants with endometrosis in the mare

Detalhes bibliográficos
Autor(a) principal: Amaral, Ana
Data de Publicação: 2020
Outros Autores: Fernandes, Carina, Faes Morazzo, Sofia, Rebordão, Maria Rosa, Szóstek-Mioduchowska, Anna, Lukasik, Karolina, Gawronska-Kozak, Barbara, Gama, Luis, Jan Skarzynski, Dariusz, Ferreira-Dias, Graça
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/24095
Resumo: Research Areas: Veterinary Sciences
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spelling The inhibition of Cathepsin G on endometrial explants with endometrosis in the mareEndometrosisCathepsin GCathepsin G inhibitorFibrosisMetallopeptidasesResearch Areas: Veterinary SciencesAlthough proteases found in neutrophil extracellular traps (NETs) have antimicrobial properties, they also stimulate collagen type 1 (COL1) production by the mare endometrium, contributing for the development of endometrosis. Cathepsin G (CAT), a protease present in NETs, is inhibited by specific inhibitors, such as cathepsin G inhibitor I (INH; beta-keto-phosphonic acid). Matrix metallopeptidases (MMPs) are proteases involved in the equilibrium of the extracellular matrix. The objective of this study was to investigate the effect of CAT and INH (a selective CAT inhibitor) on the expression of MMP-2 and MMP-9 and on gelatinolytic activity. In addition, the putative inhibitory effect of INH on CAT-induced COL1 production in mare endometrium was assessed. Endometrial explants retrieved from mares in follicular phase or midluteal phase were treated for 24 or 48 h with CAT, inhibitor alone, or both treatments. In explants, transcripts (quantitative polymerase chain reaction) of COL1A2, MMP2, and MMP9, as well as the relative abundance of COL1 protein (Western blot), and activity of MMP-2 and MMP-9 (zymography) were evaluated. The protease CAT induced COL1 expression in explants, at both estrous cycle phases and treatment times. The inhibitory effect of INH was observed on COL1A2 transcripts in follicular phase at 24-h treatment, and in midluteal phase at 48 h (P < 0.05), and on the relative abundance of COL protein in follicular phase and midluteal phase explants, at 48 h (P < 0.001). Our study suggests that MMP-2 might also be involved in an earlier response to CAT, and MMP-9 in a later response, mainly in the follicular phase. While the use of INH reduced CAT-induced COL1 endometrial expression, MMPs might be involved in the fibrogenic response to CAT. Therefore, in mare endometrium, the use of INH may be a future potential therapeutic means to reduce CAT-induced COL1 formation and to hamper endometrosis establishment.Frontiers, Media SARepositório da Universidade de LisboaAmaral, AnaFernandes, CarinaFaes Morazzo, SofiaRebordão, Maria RosaSzóstek-Mioduchowska, AnnaLukasik, KarolinaGawronska-Kozak, BarbaraGama, LuisJan Skarzynski, DariuszFerreira-Dias, Graça2022-04-18T13:40:40Z2020-10-302020-10-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24095engAmaral A, Fernandes C, Morazzo S, Rebordão MR, Szóstek-Mioduchowska A, Lukasik K, Gawronska-Kozak B, Telo da Gama L, Skarzynski DJ and Ferreira-Dias G. 2020. The inhibition of Cathepsin G on endometrial explants with endometrosis in the mare. Frontiers in Veterinary Science, 7:582211. Doi: 10.3389/fvets.2020.58221110.3389/fvets.2020.5822112297-1769info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:44Zoai:www.repository.utl.pt:10400.5/24095Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:08:13.042547Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The inhibition of Cathepsin G on endometrial explants with endometrosis in the mare
title The inhibition of Cathepsin G on endometrial explants with endometrosis in the mare
spellingShingle The inhibition of Cathepsin G on endometrial explants with endometrosis in the mare
Amaral, Ana
Endometrosis
Cathepsin G
Cathepsin G inhibitor
Fibrosis
Metallopeptidases
title_short The inhibition of Cathepsin G on endometrial explants with endometrosis in the mare
title_full The inhibition of Cathepsin G on endometrial explants with endometrosis in the mare
title_fullStr The inhibition of Cathepsin G on endometrial explants with endometrosis in the mare
title_full_unstemmed The inhibition of Cathepsin G on endometrial explants with endometrosis in the mare
title_sort The inhibition of Cathepsin G on endometrial explants with endometrosis in the mare
author Amaral, Ana
author_facet Amaral, Ana
Fernandes, Carina
Faes Morazzo, Sofia
Rebordão, Maria Rosa
Szóstek-Mioduchowska, Anna
Lukasik, Karolina
Gawronska-Kozak, Barbara
Gama, Luis
Jan Skarzynski, Dariusz
Ferreira-Dias, Graça
author_role author
author2 Fernandes, Carina
Faes Morazzo, Sofia
Rebordão, Maria Rosa
Szóstek-Mioduchowska, Anna
Lukasik, Karolina
Gawronska-Kozak, Barbara
Gama, Luis
Jan Skarzynski, Dariusz
Ferreira-Dias, Graça
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Amaral, Ana
Fernandes, Carina
Faes Morazzo, Sofia
Rebordão, Maria Rosa
Szóstek-Mioduchowska, Anna
Lukasik, Karolina
Gawronska-Kozak, Barbara
Gama, Luis
Jan Skarzynski, Dariusz
Ferreira-Dias, Graça
dc.subject.por.fl_str_mv Endometrosis
Cathepsin G
Cathepsin G inhibitor
Fibrosis
Metallopeptidases
topic Endometrosis
Cathepsin G
Cathepsin G inhibitor
Fibrosis
Metallopeptidases
description Research Areas: Veterinary Sciences
publishDate 2020
dc.date.none.fl_str_mv 2020-10-30
2020-10-30T00:00:00Z
2022-04-18T13:40:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/24095
url http://hdl.handle.net/10400.5/24095
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Amaral A, Fernandes C, Morazzo S, Rebordão MR, Szóstek-Mioduchowska A, Lukasik K, Gawronska-Kozak B, Telo da Gama L, Skarzynski DJ and Ferreira-Dias G. 2020. The inhibition of Cathepsin G on endometrial explants with endometrosis in the mare. Frontiers in Veterinary Science, 7:582211. Doi: 10.3389/fvets.2020.582211
10.3389/fvets.2020.582211
2297-1769
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers, Media SA
publisher.none.fl_str_mv Frontiers, Media SA
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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