Psd1 effects on Candida albicans planktonic cells and biofilms

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Sónia
Data de Publicação: 2017
Outros Autores: Silva, Patrícia M., Felício, Mário R., Medeiros, Luciano N. de, Kurtenbach, Eleonora, Santos, Nuno C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/33202
Resumo: Copyright © 2017 Gonçalves, Silva, Felício, de Medeiros, Kurtenbach and Santos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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spelling Psd1 effects on Candida albicans planktonic cells and biofilmsAntimicrobial peptidesAtomic force microscopyCandida albicansBiofilmConfocal microscopyCopyright © 2017 Gonçalves, Silva, Felício, de Medeiros, Kurtenbach and Santos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.Candida albicans is an important human pathogen, causing opportunistic infections. The adhesion of planktonic cells to a substrate is the first step for biofilm development. The antimicrobial peptide (AMP) Psd1 is a defensin isolated from Pisum sativum seeds. We tested the effects of this AMP on C. albicans biofilms and planktonic cells, comparing its activity with amphotericin B and fluconazole. Three C. albicans variants were studied, one of them a mutant deficient in glucosylceramide synthase, conferring resistance to Psd1 antifungal action. Atomic force microscopy (AFM) was used to assess morphological and biomechanical changes on fungal cells. Surface alterations, with membrane disruption and leakage of cellular contents, were observed. Cytometry assays and confocal microscopy imaging showed that Psd1 causes cell death, in a time and concentration-dependent manner. These results demonstrate Psd1 pleiotropic action against a relevant fungal human pathogen, suggesting its use as natural antimycotic agent.This work was funded by Fundação para a Ciência e a Tecnologia – Ministério da Ciência, Tecnologia e Ensino Superior (FCTMCTES, Portugal), FP7-IRSES project MEMPEPACROSS (European Union), Marie Skłodowska-Curie Research and Innovation Staff Exchange (MSCA-RISE) project INPACT (call H2020-MSCA-RISE-2014, grant agreement 644167, European Union) and CNPq – Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brazil). MF also acknowledges FCT-MCTES PhD fellowship SPRH/BD/100517/2014Frontiers MediaRepositório da Universidade de LisboaGonçalves, SóniaSilva, Patrícia M.Felício, Mário R.Medeiros, Luciano N. deKurtenbach, EleonoraSantos, Nuno C.2018-05-07T15:38:46Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/33202engFront. Cell. Infect. Microbiol. 7:2492235-2988doi: 10.3389/fcimb.2017.00249info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:42:33Zoai:repositorio.ul.pt:10451/33202Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:42:33Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Psd1 effects on Candida albicans planktonic cells and biofilms
title Psd1 effects on Candida albicans planktonic cells and biofilms
spellingShingle Psd1 effects on Candida albicans planktonic cells and biofilms
Gonçalves, Sónia
Antimicrobial peptides
Atomic force microscopy
Candida albicans
Biofilm
Confocal microscopy
title_short Psd1 effects on Candida albicans planktonic cells and biofilms
title_full Psd1 effects on Candida albicans planktonic cells and biofilms
title_fullStr Psd1 effects on Candida albicans planktonic cells and biofilms
title_full_unstemmed Psd1 effects on Candida albicans planktonic cells and biofilms
title_sort Psd1 effects on Candida albicans planktonic cells and biofilms
author Gonçalves, Sónia
author_facet Gonçalves, Sónia
Silva, Patrícia M.
Felício, Mário R.
Medeiros, Luciano N. de
Kurtenbach, Eleonora
Santos, Nuno C.
author_role author
author2 Silva, Patrícia M.
Felício, Mário R.
Medeiros, Luciano N. de
Kurtenbach, Eleonora
Santos, Nuno C.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Gonçalves, Sónia
Silva, Patrícia M.
Felício, Mário R.
Medeiros, Luciano N. de
Kurtenbach, Eleonora
Santos, Nuno C.
dc.subject.por.fl_str_mv Antimicrobial peptides
Atomic force microscopy
Candida albicans
Biofilm
Confocal microscopy
topic Antimicrobial peptides
Atomic force microscopy
Candida albicans
Biofilm
Confocal microscopy
description Copyright © 2017 Gonçalves, Silva, Felício, de Medeiros, Kurtenbach and Santos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2018-05-07T15:38:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/33202
url http://hdl.handle.net/10451/33202
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Front. Cell. Infect. Microbiol. 7:249
2235-2988
doi: 10.3389/fcimb.2017.00249
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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