Transport and utilization of hexoses and pentoses in the halotolerant yeast Debaryomyces hansenii
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/2791 |
Resumo: | Debaryomyces hansenii is a yeast species well known for its halotolerance. It has seldom been mentioned as a pentose consumer. In the present work, a strain of this species was investigated with respect to the utilization of mixtures of pentoses and hexoses. Growth parameters were calculated from batch aerobic cultures with pentoses, hexoses and mixtures of both sugars as carbon sources. Growth on pentoses was slower than on hexoses, but the values obtained for biomass yields were very similar in both type of sugars. Furthermore, in mixtures of both sugars, the preference for one carbon source does not inhibit consumption of the other. Glucose and xylose were transported by cells grown on glucose, via a specific low-affinity facilitated diffusion system. Cells derepressed by growth on xylose exhibiteded two distinct high-affinity transport systems for glucose and xylose. The characterization of these transport systems allowed us to consider them as proton symports, although they displayed sugar associated proton uptake exclusively only in the presence of NaCl or KCl. When the individual sugar uptake rates during cultivation and the Vmax of transport systems for glucose and xylose were compared, transport appeared not to limit the growth rate on either sugar. |
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Transport and utilization of hexoses and pentoses in the halotolerant yeast Debaryomyces hanseniiDebaryomyces hanseniiXylose utilisationSugar transportSugar mixturesScience & TechnologyDebaryomyces hansenii is a yeast species well known for its halotolerance. It has seldom been mentioned as a pentose consumer. In the present work, a strain of this species was investigated with respect to the utilization of mixtures of pentoses and hexoses. Growth parameters were calculated from batch aerobic cultures with pentoses, hexoses and mixtures of both sugars as carbon sources. Growth on pentoses was slower than on hexoses, but the values obtained for biomass yields were very similar in both type of sugars. Furthermore, in mixtures of both sugars, the preference for one carbon source does not inhibit consumption of the other. Glucose and xylose were transported by cells grown on glucose, via a specific low-affinity facilitated diffusion system. Cells derepressed by growth on xylose exhibiteded two distinct high-affinity transport systems for glucose and xylose. The characterization of these transport systems allowed us to consider them as proton symports, although they displayed sugar associated proton uptake exclusively only in the presence of NaCl or KCl. When the individual sugar uptake rates during cultivation and the Vmax of transport systems for glucose and xylose were compared, transport appeared not to limit the growth rate on either sugar.American Society for Microbiology (ASM)Universidade do MinhoNobre, AlexandraLucas, CândidaLeão, Cecília19991999-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/2791eng“Applied and environmental microbiology”. ISSN 0099-2240. 65:8 (1999) 3594-3598.0099-22401098-533610.1128/AEM.65.8.3594-3598.199910427054https://aem.asm.org/content/65/8/3594info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:35:56Zoai:repositorium.sdum.uminho.pt:1822/2791Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:35:56Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Transport and utilization of hexoses and pentoses in the halotolerant yeast Debaryomyces hansenii |
title |
Transport and utilization of hexoses and pentoses in the halotolerant yeast Debaryomyces hansenii |
spellingShingle |
Transport and utilization of hexoses and pentoses in the halotolerant yeast Debaryomyces hansenii Nobre, Alexandra Debaryomyces hansenii Xylose utilisation Sugar transport Sugar mixtures Science & Technology |
title_short |
Transport and utilization of hexoses and pentoses in the halotolerant yeast Debaryomyces hansenii |
title_full |
Transport and utilization of hexoses and pentoses in the halotolerant yeast Debaryomyces hansenii |
title_fullStr |
Transport and utilization of hexoses and pentoses in the halotolerant yeast Debaryomyces hansenii |
title_full_unstemmed |
Transport and utilization of hexoses and pentoses in the halotolerant yeast Debaryomyces hansenii |
title_sort |
Transport and utilization of hexoses and pentoses in the halotolerant yeast Debaryomyces hansenii |
author |
Nobre, Alexandra |
author_facet |
Nobre, Alexandra Lucas, Cândida Leão, Cecília |
author_role |
author |
author2 |
Lucas, Cândida Leão, Cecília |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Nobre, Alexandra Lucas, Cândida Leão, Cecília |
dc.subject.por.fl_str_mv |
Debaryomyces hansenii Xylose utilisation Sugar transport Sugar mixtures Science & Technology |
topic |
Debaryomyces hansenii Xylose utilisation Sugar transport Sugar mixtures Science & Technology |
description |
Debaryomyces hansenii is a yeast species well known for its halotolerance. It has seldom been mentioned as a pentose consumer. In the present work, a strain of this species was investigated with respect to the utilization of mixtures of pentoses and hexoses. Growth parameters were calculated from batch aerobic cultures with pentoses, hexoses and mixtures of both sugars as carbon sources. Growth on pentoses was slower than on hexoses, but the values obtained for biomass yields were very similar in both type of sugars. Furthermore, in mixtures of both sugars, the preference for one carbon source does not inhibit consumption of the other. Glucose and xylose were transported by cells grown on glucose, via a specific low-affinity facilitated diffusion system. Cells derepressed by growth on xylose exhibiteded two distinct high-affinity transport systems for glucose and xylose. The characterization of these transport systems allowed us to consider them as proton symports, although they displayed sugar associated proton uptake exclusively only in the presence of NaCl or KCl. When the individual sugar uptake rates during cultivation and the Vmax of transport systems for glucose and xylose were compared, transport appeared not to limit the growth rate on either sugar. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999 1999-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/2791 |
url |
http://hdl.handle.net/1822/2791 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
“Applied and environmental microbiology”. ISSN 0099-2240. 65:8 (1999) 3594-3598. 0099-2240 1098-5336 10.1128/AEM.65.8.3594-3598.1999 10427054 https://aem.asm.org/content/65/8/3594 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology (ASM) |
publisher.none.fl_str_mv |
American Society for Microbiology (ASM) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
_version_ |
1817545027651371008 |