Genomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strains

Detalhes bibliográficos
Autor(a) principal: Silva, Marta Filipa Serra da
Data de Publicação: 2021
Outros Autores: Pereira, Ana L., Fraqueza, M. J., Pereira, Gonçalo, Mateus, Luisa, Lopes-da-Costa, Luís, Silva, E
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/23440
Resumo: Research Areas: Microbiology
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spelling Genomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strainsCampylobacter fetus subsp. venerealisBiovar intermediusMultilocus sequencing typingVirulence potentialAntimicrobial susceptibilityGenomic characterizationResearch Areas: MicrobiologyThe pathogenesis mechanisms of Campylobacter fetus subsp. venerealis (Cfv), the etiologic agent of Bovine Genital Campylobacteriosis remain elusive. This study evaluated the virulence potential and biovar characteristics of Cfv isolates (n = 13) by PCR screening of putative virulencefactor (VF) genes, Multilocus Sequence Typing (MLST) analysis, antimicrobial susceptibility to tetracycline, penicillin, enrofloxacin and streptomycin testing and whole-genome sequencing (WGS; n = 5), also comparing the latter with 26 other whole-genome sequences of Cfv strains. The putative VF genes encoding type IV secretion system of Cfv (virB2-virB11/virD4) were absent in 92% of isolates, including isolates from aborted foetuses, evidencing that these VF genes are not essential for Cfv pathogenicity. The parA gene, used as a Cfv diagnostic molecular target, was detected in only 3 of 13 isolates, invalidating its use for diagnosis purposes. Three novel sequence types were identified by MLST. Although no in vitro antimicrobial resistance was detected, WGS identified antimicrobial resistance-related genes, including those encoding the multidrug efflux pumps CmeABC and YkkCD, indicating that their presence is not enough to provide antimicrobial resistance. The SNP and accessory protein families analysis segregated the Cfv and Cfv biovar intermedius (Cfvi) strains into different clusters. In conclusion, this study evidenced virulence potential and biovar characteristics of Cfv and Cfvi, which are of relevance for the control of Bovine Genital Campylobacteriosis.MDPIRepositório da Universidade de LisboaSilva, Marta Filipa Serra daPereira, Ana L.Fraqueza, M. J.Pereira, GonçaloMateus, LuisaLopes-da-Costa, LuísSilva, E2022-02-08T23:21:51Z2021-02-092021-02-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23440engSilva MF, Pereira AL, Fraqueza MJ, Pereira G, Mateus L, Lopes-da-Costa L, Silva E. 2021. Genomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strains. Microorganisms, 9(2):340. Doi: 10.3390/microorganisms902034010.3390/microorganisms90203402076-2607info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:01Zoai:www.repository.utl.pt:10400.5/23440Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:40.830801Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strains
title Genomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strains
spellingShingle Genomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strains
Silva, Marta Filipa Serra da
Campylobacter fetus subsp. venerealis
Biovar intermedius
Multilocus sequencing typing
Virulence potential
Antimicrobial susceptibility
Genomic characterization
title_short Genomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strains
title_full Genomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strains
title_fullStr Genomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strains
title_full_unstemmed Genomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strains
title_sort Genomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strains
author Silva, Marta Filipa Serra da
author_facet Silva, Marta Filipa Serra da
Pereira, Ana L.
Fraqueza, M. J.
Pereira, Gonçalo
Mateus, Luisa
Lopes-da-Costa, Luís
Silva, E
author_role author
author2 Pereira, Ana L.
Fraqueza, M. J.
Pereira, Gonçalo
Mateus, Luisa
Lopes-da-Costa, Luís
Silva, E
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Silva, Marta Filipa Serra da
Pereira, Ana L.
Fraqueza, M. J.
Pereira, Gonçalo
Mateus, Luisa
Lopes-da-Costa, Luís
Silva, E
dc.subject.por.fl_str_mv Campylobacter fetus subsp. venerealis
Biovar intermedius
Multilocus sequencing typing
Virulence potential
Antimicrobial susceptibility
Genomic characterization
topic Campylobacter fetus subsp. venerealis
Biovar intermedius
Multilocus sequencing typing
Virulence potential
Antimicrobial susceptibility
Genomic characterization
description Research Areas: Microbiology
publishDate 2021
dc.date.none.fl_str_mv 2021-02-09
2021-02-09T00:00:00Z
2022-02-08T23:21:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/23440
url http://hdl.handle.net/10400.5/23440
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva MF, Pereira AL, Fraqueza MJ, Pereira G, Mateus L, Lopes-da-Costa L, Silva E. 2021. Genomic and phenotypic characterization of Campylobacter fetus subsp. venerealis strains. Microorganisms, 9(2):340. Doi: 10.3390/microorganisms9020340
10.3390/microorganisms9020340
2076-2607
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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