How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/8930 |
Resumo: | The capabilities of bioanalytical mass spectrometry to (i) detect and differentiate viruses at the peptide level whilst maintaining high sample throughput and (ii) to provide diagnosis and prognosis for infected patients are presented as a tutorial in this work to aid analytical chemists and physicians to gain insights into the possibilities offered by current high-resolution mass spectrometry technology and bioinformatics. From (i) sampling to sample treatment; (ii) Matrix-Assisted Laser Desorption Ionization- to Electrospray Ionization -based mass spectrometry; and (iii) from clustering to peptide sequencing; a detailed step-by-step guide is provided and exemplified using SARS-CoV-2 Spike Y839 variant and the variant of concern SARS-CoV-2 Alpha (B.1.1.7 lineage), Influenza B, and Influenza A subtypes AH1N1pdm09 and AH3N2. |
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How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorialImmuno-mass SpectrometryViral DetectionMALDI-TOF-MSLC-ESI-Q-TOF-MS/MSInfecções RespiratóriasThe capabilities of bioanalytical mass spectrometry to (i) detect and differentiate viruses at the peptide level whilst maintaining high sample throughput and (ii) to provide diagnosis and prognosis for infected patients are presented as a tutorial in this work to aid analytical chemists and physicians to gain insights into the possibilities offered by current high-resolution mass spectrometry technology and bioinformatics. From (i) sampling to sample treatment; (ii) Matrix-Assisted Laser Desorption Ionization- to Electrospray Ionization -based mass spectrometry; and (iii) from clustering to peptide sequencing; a detailed step-by-step guide is provided and exemplified using SARS-CoV-2 Spike Y839 variant and the variant of concern SARS-CoV-2 Alpha (B.1.1.7 lineage), Influenza B, and Influenza A subtypes AH1N1pdm09 and AH3N2.Highlights: - Immunohistochemistry with magnetic core nanoparticles to isolate viruses. - The use of MALDI-MS for rapid virus detection is explained in detail; - The use of ESI-MS/MS to pinpoint host-patient crosstalk is explained in detail. - The absolute quantitative MS is explained for large-scale protein quantitation.This work received financial support from PT national funds (FCT/MCTES) through the projects UIDB/50006/2020 and UIDP/50006/2020 and from PROTEOMASS Scientific Society through the projects #PM001/2019 and #PM003/2016.ElsevierRepositório Científico do Instituto Nacional de SaúdeSantos, Hugo M.Carvalho, Luís B.Lodeiro, CarlosMartins, GonçaloGomes, Inês L.Antunes, Wilson D.T.Correia, VanessaAlmeida-Santos, Maria M.Rebelo-de-Andrade, HelenaMatos, António P.A.Capelo, J.L.2024-01-18T14:22:41Z2023-032023-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8930engMicrochem J. 2023 Mar;186:108323. doi:10.1016/j.microc.2022.108323. Epub 2022 Dec160026-265X10.1016/j.microc.2022.108323info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-20T01:32:35Zoai:repositorio.insa.pt:10400.18/8930Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:52:21.322045Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial |
title |
How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial |
spellingShingle |
How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial Santos, Hugo M. Immuno-mass Spectrometry Viral Detection MALDI-TOF-MS LC-ESI-Q-TOF-MS/MS Infecções Respiratórias |
title_short |
How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial |
title_full |
How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial |
title_fullStr |
How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial |
title_full_unstemmed |
How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial |
title_sort |
How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial |
author |
Santos, Hugo M. |
author_facet |
Santos, Hugo M. Carvalho, Luís B. Lodeiro, Carlos Martins, Gonçalo Gomes, Inês L. Antunes, Wilson D.T. Correia, Vanessa Almeida-Santos, Maria M. Rebelo-de-Andrade, Helena Matos, António P.A. Capelo, J.L. |
author_role |
author |
author2 |
Carvalho, Luís B. Lodeiro, Carlos Martins, Gonçalo Gomes, Inês L. Antunes, Wilson D.T. Correia, Vanessa Almeida-Santos, Maria M. Rebelo-de-Andrade, Helena Matos, António P.A. Capelo, J.L. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Santos, Hugo M. Carvalho, Luís B. Lodeiro, Carlos Martins, Gonçalo Gomes, Inês L. Antunes, Wilson D.T. Correia, Vanessa Almeida-Santos, Maria M. Rebelo-de-Andrade, Helena Matos, António P.A. Capelo, J.L. |
dc.subject.por.fl_str_mv |
Immuno-mass Spectrometry Viral Detection MALDI-TOF-MS LC-ESI-Q-TOF-MS/MS Infecções Respiratórias |
topic |
Immuno-mass Spectrometry Viral Detection MALDI-TOF-MS LC-ESI-Q-TOF-MS/MS Infecções Respiratórias |
description |
The capabilities of bioanalytical mass spectrometry to (i) detect and differentiate viruses at the peptide level whilst maintaining high sample throughput and (ii) to provide diagnosis and prognosis for infected patients are presented as a tutorial in this work to aid analytical chemists and physicians to gain insights into the possibilities offered by current high-resolution mass spectrometry technology and bioinformatics. From (i) sampling to sample treatment; (ii) Matrix-Assisted Laser Desorption Ionization- to Electrospray Ionization -based mass spectrometry; and (iii) from clustering to peptide sequencing; a detailed step-by-step guide is provided and exemplified using SARS-CoV-2 Spike Y839 variant and the variant of concern SARS-CoV-2 Alpha (B.1.1.7 lineage), Influenza B, and Influenza A subtypes AH1N1pdm09 and AH3N2. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03 2023-03-01T00:00:00Z 2024-01-18T14:22:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/8930 |
url |
http://hdl.handle.net/10400.18/8930 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microchem J. 2023 Mar;186:108323. doi:10.1016/j.microc.2022.108323. Epub 2022 Dec16 0026-265X 10.1016/j.microc.2022.108323 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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