How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial

Detalhes bibliográficos
Autor(a) principal: Santos, Hugo M.
Data de Publicação: 2023
Outros Autores: Carvalho, Luís B., Lodeiro, Carlos, Martins, Gonçalo, Gomes, Inês L., Antunes, Wilson D.T., Correia, Vanessa, Almeida-Santos, Maria M., Rebelo-de-Andrade, Helena, Matos, António P.A., Capelo, J.L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8930
Resumo: The capabilities of bioanalytical mass spectrometry to (i) detect and differentiate viruses at the peptide level whilst maintaining high sample throughput and (ii) to provide diagnosis and prognosis for infected patients are presented as a tutorial in this work to aid analytical chemists and physicians to gain insights into the possibilities offered by current high-resolution mass spectrometry technology and bioinformatics. From (i) sampling to sample treatment; (ii) Matrix-Assisted Laser Desorption Ionization- to Electrospray Ionization -based mass spectrometry; and (iii) from clustering to peptide sequencing; a detailed step-by-step guide is provided and exemplified using SARS-CoV-2 Spike Y839 variant and the variant of concern SARS-CoV-2 Alpha (B.1.1.7 lineage), Influenza B, and Influenza A subtypes AH1N1pdm09 and AH3N2.
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spelling How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorialImmuno-mass SpectrometryViral DetectionMALDI-TOF-MSLC-ESI-Q-TOF-MS/MSInfecções RespiratóriasThe capabilities of bioanalytical mass spectrometry to (i) detect and differentiate viruses at the peptide level whilst maintaining high sample throughput and (ii) to provide diagnosis and prognosis for infected patients are presented as a tutorial in this work to aid analytical chemists and physicians to gain insights into the possibilities offered by current high-resolution mass spectrometry technology and bioinformatics. From (i) sampling to sample treatment; (ii) Matrix-Assisted Laser Desorption Ionization- to Electrospray Ionization -based mass spectrometry; and (iii) from clustering to peptide sequencing; a detailed step-by-step guide is provided and exemplified using SARS-CoV-2 Spike Y839 variant and the variant of concern SARS-CoV-2 Alpha (B.1.1.7 lineage), Influenza B, and Influenza A subtypes AH1N1pdm09 and AH3N2.Highlights: - Immunohistochemistry with magnetic core nanoparticles to isolate viruses. - The use of MALDI-MS for rapid virus detection is explained in detail; - The use of ESI-MS/MS to pinpoint host-patient crosstalk is explained in detail. - The absolute quantitative MS is explained for large-scale protein quantitation.This work received financial support from PT national funds (FCT/MCTES) through the projects UIDB/50006/2020 and UIDP/50006/2020 and from PROTEOMASS Scientific Society through the projects #PM001/2019 and #PM003/2016.ElsevierRepositório Científico do Instituto Nacional de SaúdeSantos, Hugo M.Carvalho, Luís B.Lodeiro, CarlosMartins, GonçaloGomes, Inês L.Antunes, Wilson D.T.Correia, VanessaAlmeida-Santos, Maria M.Rebelo-de-Andrade, HelenaMatos, António P.A.Capelo, J.L.2024-01-18T14:22:41Z2023-032023-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8930engMicrochem J. 2023 Mar;186:108323. doi:10.1016/j.microc.2022.108323. Epub 2022 Dec160026-265X10.1016/j.microc.2022.108323info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-20T01:32:35Zoai:repositorio.insa.pt:10400.18/8930Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:52:21.322045Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial
title How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial
spellingShingle How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial
Santos, Hugo M.
Immuno-mass Spectrometry
Viral Detection
MALDI-TOF-MS
LC-ESI-Q-TOF-MS/MS
Infecções Respiratórias
title_short How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial
title_full How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial
title_fullStr How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial
title_full_unstemmed How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial
title_sort How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial
author Santos, Hugo M.
author_facet Santos, Hugo M.
Carvalho, Luís B.
Lodeiro, Carlos
Martins, Gonçalo
Gomes, Inês L.
Antunes, Wilson D.T.
Correia, Vanessa
Almeida-Santos, Maria M.
Rebelo-de-Andrade, Helena
Matos, António P.A.
Capelo, J.L.
author_role author
author2 Carvalho, Luís B.
Lodeiro, Carlos
Martins, Gonçalo
Gomes, Inês L.
Antunes, Wilson D.T.
Correia, Vanessa
Almeida-Santos, Maria M.
Rebelo-de-Andrade, Helena
Matos, António P.A.
Capelo, J.L.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Santos, Hugo M.
Carvalho, Luís B.
Lodeiro, Carlos
Martins, Gonçalo
Gomes, Inês L.
Antunes, Wilson D.T.
Correia, Vanessa
Almeida-Santos, Maria M.
Rebelo-de-Andrade, Helena
Matos, António P.A.
Capelo, J.L.
dc.subject.por.fl_str_mv Immuno-mass Spectrometry
Viral Detection
MALDI-TOF-MS
LC-ESI-Q-TOF-MS/MS
Infecções Respiratórias
topic Immuno-mass Spectrometry
Viral Detection
MALDI-TOF-MS
LC-ESI-Q-TOF-MS/MS
Infecções Respiratórias
description The capabilities of bioanalytical mass spectrometry to (i) detect and differentiate viruses at the peptide level whilst maintaining high sample throughput and (ii) to provide diagnosis and prognosis for infected patients are presented as a tutorial in this work to aid analytical chemists and physicians to gain insights into the possibilities offered by current high-resolution mass spectrometry technology and bioinformatics. From (i) sampling to sample treatment; (ii) Matrix-Assisted Laser Desorption Ionization- to Electrospray Ionization -based mass spectrometry; and (iii) from clustering to peptide sequencing; a detailed step-by-step guide is provided and exemplified using SARS-CoV-2 Spike Y839 variant and the variant of concern SARS-CoV-2 Alpha (B.1.1.7 lineage), Influenza B, and Influenza A subtypes AH1N1pdm09 and AH3N2.
publishDate 2023
dc.date.none.fl_str_mv 2023-03
2023-03-01T00:00:00Z
2024-01-18T14:22:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8930
url http://hdl.handle.net/10400.18/8930
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microchem J. 2023 Mar;186:108323. doi:10.1016/j.microc.2022.108323. Epub 2022 Dec16
0026-265X
10.1016/j.microc.2022.108323
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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