Spontaneous one-lung ventilation increases the lung inflammatory response : an experimental pilot study
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.2/3741 |
Resumo: | Study objective: The purpose of this study was to investigate if spontaneous one-lung ventilation would induce any type of inflammatory lung response when compared to spontaneous two-lung ventilation and its intensity, by quantification of inflammatory cells in lung histology at the end of the procedure. Design: In vivo prospective randomised animal study Setting: University research laboratory Subjects: New Zealand rabbits Interventions: Rabbits (n=20) were randomly assigned to 4 groups (n=5 each group). Groups 1 and 2 were submitted to one-lung ventilation, during 20 and 75 minutes respectively; groups 3 and 4 were submitted to two-lung ventilation during 20 and 75 minutes and considered controls. Ketamine/xylazine was administered for induction and maintenance of anesthesia. One-lung ventilation was achieved by administration of air into the interpleural space, and left lung collapse was visually confirmed through the centre of the diaphragm. Measurements: Lung histology preparations were observed under light microscopy for quantification of the inflammatory response (light, moderate and severe). Main results: All subjects had at least light inflammatory response. However, rabbits submitted to one-lung ventilation had a statistically significant value for the occurrence of moderate inflammation (p<0.05). The inflammatory response found included mainly eosinophils, with an average proportion of 75/25 to other polymorphonuclear cells. No differences between groups were found regarding gas exchange, heart rate and respiratory rate. Conclusions: In this spontaneous one-lung ventilation model, lung collapse was positively associated with a greater inflammatory response when compared to normal two-lung ventilation. |
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Spontaneous one-lung ventilation increases the lung inflammatory response : an experimental pilot studyInflammatory responseOne-lung ventilationAtelectasisStudy objective: The purpose of this study was to investigate if spontaneous one-lung ventilation would induce any type of inflammatory lung response when compared to spontaneous two-lung ventilation and its intensity, by quantification of inflammatory cells in lung histology at the end of the procedure. Design: In vivo prospective randomised animal study Setting: University research laboratory Subjects: New Zealand rabbits Interventions: Rabbits (n=20) were randomly assigned to 4 groups (n=5 each group). Groups 1 and 2 were submitted to one-lung ventilation, during 20 and 75 minutes respectively; groups 3 and 4 were submitted to two-lung ventilation during 20 and 75 minutes and considered controls. Ketamine/xylazine was administered for induction and maintenance of anesthesia. One-lung ventilation was achieved by administration of air into the interpleural space, and left lung collapse was visually confirmed through the centre of the diaphragm. Measurements: Lung histology preparations were observed under light microscopy for quantification of the inflammatory response (light, moderate and severe). Main results: All subjects had at least light inflammatory response. However, rabbits submitted to one-lung ventilation had a statistically significant value for the occurrence of moderate inflammation (p<0.05). The inflammatory response found included mainly eosinophils, with an average proportion of 75/25 to other polymorphonuclear cells. No differences between groups were found regarding gas exchange, heart rate and respiratory rate. Conclusions: In this spontaneous one-lung ventilation model, lung collapse was positively associated with a greater inflammatory response when compared to normal two-lung ventilation.OMICS InternationalRepositório AbertoMachado, Humberto S.Sá, PaulaNunes, Catarina S.Couceiro, AntónioSilva, Álvaro Moreira daÁguas, Artur2015-03-03T11:35:47Z2014-08-112014-08-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.2/3741engMachado, Humberto S. [et al.] - Spontaneous one-lung ventilation increases the lung inflammatory response : an experimental pilot study. "Journal of Anesthesia & Clinical Research" [Em linha]. ISSN 2155-6148. Vol. 8, nº 5 (2014), p. 1-52155-614810.4172/2155-6148.1000428info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-16T15:19:04Zoai:repositorioaberto.uab.pt:10400.2/3741Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:44:57.183719Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Spontaneous one-lung ventilation increases the lung inflammatory response : an experimental pilot study |
title |
Spontaneous one-lung ventilation increases the lung inflammatory response : an experimental pilot study |
spellingShingle |
Spontaneous one-lung ventilation increases the lung inflammatory response : an experimental pilot study Machado, Humberto S. Inflammatory response One-lung ventilation Atelectasis |
title_short |
Spontaneous one-lung ventilation increases the lung inflammatory response : an experimental pilot study |
title_full |
Spontaneous one-lung ventilation increases the lung inflammatory response : an experimental pilot study |
title_fullStr |
Spontaneous one-lung ventilation increases the lung inflammatory response : an experimental pilot study |
title_full_unstemmed |
Spontaneous one-lung ventilation increases the lung inflammatory response : an experimental pilot study |
title_sort |
Spontaneous one-lung ventilation increases the lung inflammatory response : an experimental pilot study |
author |
Machado, Humberto S. |
author_facet |
Machado, Humberto S. Sá, Paula Nunes, Catarina S. Couceiro, António Silva, Álvaro Moreira da Águas, Artur |
author_role |
author |
author2 |
Sá, Paula Nunes, Catarina S. Couceiro, António Silva, Álvaro Moreira da Águas, Artur |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Aberto |
dc.contributor.author.fl_str_mv |
Machado, Humberto S. Sá, Paula Nunes, Catarina S. Couceiro, António Silva, Álvaro Moreira da Águas, Artur |
dc.subject.por.fl_str_mv |
Inflammatory response One-lung ventilation Atelectasis |
topic |
Inflammatory response One-lung ventilation Atelectasis |
description |
Study objective: The purpose of this study was to investigate if spontaneous one-lung ventilation would induce any type of inflammatory lung response when compared to spontaneous two-lung ventilation and its intensity, by quantification of inflammatory cells in lung histology at the end of the procedure. Design: In vivo prospective randomised animal study Setting: University research laboratory Subjects: New Zealand rabbits Interventions: Rabbits (n=20) were randomly assigned to 4 groups (n=5 each group). Groups 1 and 2 were submitted to one-lung ventilation, during 20 and 75 minutes respectively; groups 3 and 4 were submitted to two-lung ventilation during 20 and 75 minutes and considered controls. Ketamine/xylazine was administered for induction and maintenance of anesthesia. One-lung ventilation was achieved by administration of air into the interpleural space, and left lung collapse was visually confirmed through the centre of the diaphragm. Measurements: Lung histology preparations were observed under light microscopy for quantification of the inflammatory response (light, moderate and severe). Main results: All subjects had at least light inflammatory response. However, rabbits submitted to one-lung ventilation had a statistically significant value for the occurrence of moderate inflammation (p<0.05). The inflammatory response found included mainly eosinophils, with an average proportion of 75/25 to other polymorphonuclear cells. No differences between groups were found regarding gas exchange, heart rate and respiratory rate. Conclusions: In this spontaneous one-lung ventilation model, lung collapse was positively associated with a greater inflammatory response when compared to normal two-lung ventilation. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08-11 2014-08-11T00:00:00Z 2015-03-03T11:35:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.2/3741 |
url |
http://hdl.handle.net/10400.2/3741 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Machado, Humberto S. [et al.] - Spontaneous one-lung ventilation increases the lung inflammatory response : an experimental pilot study. "Journal of Anesthesia & Clinical Research" [Em linha]. ISSN 2155-6148. Vol. 8, nº 5 (2014), p. 1-5 2155-6148 10.4172/2155-6148.1000428 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
OMICS International |
publisher.none.fl_str_mv |
OMICS International |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799135020253708288 |