Ibuprofen-loaded fibrous patches-taming inhibition at the spinal cord injury site
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/120735 |
Resumo: | It is now widely accepted that a therapeutic strategy for spinal cord injury (SCI) demands a multi-target approach. Here we propose the use of an easily implantable bilayer polymeric patch based on poly(trimethylene carbonate-co-e-caprolactone) (P(TMC-CL)) that combines physical guidance cues provided by electrospun aligned fibres and the delivery of ibuprofen, as a mean to reduce the inhibitory environment at the lesion site by taming RhoA activation. Bilayer patches comprised a solvent cast film onto which electrospun aligned fibres have been deposited. Both layers were loaded with ibuprofen. In vitro release (37°C, in phosphate buffered saline) of the drug from the loaded scaffolds under sink condition was found to occur in the first 24 h. The released ibuprofen was shown to retain its bioactivity, as indicated by the reduction of RhoA activation when the neuronal-like cell line ND7/23 was challenged with lysophosphatidic acid. Ibuprofen-loaded P(TMC-CL) bilayer scaffolds were successfully implanted in vivo in a dorsal hemisection rat SCI model mediating the reduction of RhoA activation after 5 days of implantation in comparison to plain P(TMC-CL) scaffolds. Immunohistochemical analysis of the tissue shows ßIII tubulin positive cells close to the ibuprofen-loaded patches further supporting the use of this strategy in the context of regeneration after a lesion in the spinal cord. |
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Ibuprofen-loaded fibrous patches-taming inhibition at the spinal cord injury siteAnimalsCells, CulturedDioxanes/chemistryDrug Carriers/chemistryDrug Delivery Systems/methodsIbuprofen/administration & dosageMiceMicrotechnologyNanofibers/chemistryNerve Regeneration/drug effectsNerve Regeneration/physiologyPolyesters/chemistryPolymers/chemistryRatsSpinal Cord/drug effectsSpinal Cord/physiologySpinal Cord Injuries/therapyTissue EngineeringTissue Scaffolds/chemistryTransdermal PatchIt is now widely accepted that a therapeutic strategy for spinal cord injury (SCI) demands a multi-target approach. Here we propose the use of an easily implantable bilayer polymeric patch based on poly(trimethylene carbonate-co-e-caprolactone) (P(TMC-CL)) that combines physical guidance cues provided by electrospun aligned fibres and the delivery of ibuprofen, as a mean to reduce the inhibitory environment at the lesion site by taming RhoA activation. Bilayer patches comprised a solvent cast film onto which electrospun aligned fibres have been deposited. Both layers were loaded with ibuprofen. In vitro release (37°C, in phosphate buffered saline) of the drug from the loaded scaffolds under sink condition was found to occur in the first 24 h. The released ibuprofen was shown to retain its bioactivity, as indicated by the reduction of RhoA activation when the neuronal-like cell line ND7/23 was challenged with lysophosphatidic acid. Ibuprofen-loaded P(TMC-CL) bilayer scaffolds were successfully implanted in vivo in a dorsal hemisection rat SCI model mediating the reduction of RhoA activation after 5 days of implantation in comparison to plain P(TMC-CL) scaffolds. Immunohistochemical analysis of the tissue shows ßIII tubulin positive cells close to the ibuprofen-loaded patches further supporting the use of this strategy in the context of regeneration after a lesion in the spinal cord.Springer Verlag20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/120735eng0957-453010.1007/s10856-017-5967-7Pires, LLopes, CSalvador, DRocha, DNPêgo, APinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T13:54:12Zoai:repositorio-aberto.up.pt:10216/120735Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:50:16.993465Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ibuprofen-loaded fibrous patches-taming inhibition at the spinal cord injury site |
title |
Ibuprofen-loaded fibrous patches-taming inhibition at the spinal cord injury site |
spellingShingle |
Ibuprofen-loaded fibrous patches-taming inhibition at the spinal cord injury site Pires, L Animals Cells, Cultured Dioxanes/chemistry Drug Carriers/chemistry Drug Delivery Systems/methods Ibuprofen/administration & dosage Mice Microtechnology Nanofibers/chemistry Nerve Regeneration/drug effects Nerve Regeneration/physiology Polyesters/chemistry Polymers/chemistry Rats Spinal Cord/drug effects Spinal Cord/physiology Spinal Cord Injuries/therapy Tissue Engineering Tissue Scaffolds/chemistry Transdermal Patch |
title_short |
Ibuprofen-loaded fibrous patches-taming inhibition at the spinal cord injury site |
title_full |
Ibuprofen-loaded fibrous patches-taming inhibition at the spinal cord injury site |
title_fullStr |
Ibuprofen-loaded fibrous patches-taming inhibition at the spinal cord injury site |
title_full_unstemmed |
Ibuprofen-loaded fibrous patches-taming inhibition at the spinal cord injury site |
title_sort |
Ibuprofen-loaded fibrous patches-taming inhibition at the spinal cord injury site |
author |
Pires, L |
author_facet |
Pires, L Lopes, C Salvador, D Rocha, DN Pêgo, AP |
author_role |
author |
author2 |
Lopes, C Salvador, D Rocha, DN Pêgo, AP |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pires, L Lopes, C Salvador, D Rocha, DN Pêgo, AP |
dc.subject.por.fl_str_mv |
Animals Cells, Cultured Dioxanes/chemistry Drug Carriers/chemistry Drug Delivery Systems/methods Ibuprofen/administration & dosage Mice Microtechnology Nanofibers/chemistry Nerve Regeneration/drug effects Nerve Regeneration/physiology Polyesters/chemistry Polymers/chemistry Rats Spinal Cord/drug effects Spinal Cord/physiology Spinal Cord Injuries/therapy Tissue Engineering Tissue Scaffolds/chemistry Transdermal Patch |
topic |
Animals Cells, Cultured Dioxanes/chemistry Drug Carriers/chemistry Drug Delivery Systems/methods Ibuprofen/administration & dosage Mice Microtechnology Nanofibers/chemistry Nerve Regeneration/drug effects Nerve Regeneration/physiology Polyesters/chemistry Polymers/chemistry Rats Spinal Cord/drug effects Spinal Cord/physiology Spinal Cord Injuries/therapy Tissue Engineering Tissue Scaffolds/chemistry Transdermal Patch |
description |
It is now widely accepted that a therapeutic strategy for spinal cord injury (SCI) demands a multi-target approach. Here we propose the use of an easily implantable bilayer polymeric patch based on poly(trimethylene carbonate-co-e-caprolactone) (P(TMC-CL)) that combines physical guidance cues provided by electrospun aligned fibres and the delivery of ibuprofen, as a mean to reduce the inhibitory environment at the lesion site by taming RhoA activation. Bilayer patches comprised a solvent cast film onto which electrospun aligned fibres have been deposited. Both layers were loaded with ibuprofen. In vitro release (37°C, in phosphate buffered saline) of the drug from the loaded scaffolds under sink condition was found to occur in the first 24 h. The released ibuprofen was shown to retain its bioactivity, as indicated by the reduction of RhoA activation when the neuronal-like cell line ND7/23 was challenged with lysophosphatidic acid. Ibuprofen-loaded P(TMC-CL) bilayer scaffolds were successfully implanted in vivo in a dorsal hemisection rat SCI model mediating the reduction of RhoA activation after 5 days of implantation in comparison to plain P(TMC-CL) scaffolds. Immunohistochemical analysis of the tissue shows ßIII tubulin positive cells close to the ibuprofen-loaded patches further supporting the use of this strategy in the context of regeneration after a lesion in the spinal cord. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/120735 |
url |
https://hdl.handle.net/10216/120735 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0957-4530 10.1007/s10856-017-5967-7 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Verlag |
publisher.none.fl_str_mv |
Springer Verlag |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799135822609383425 |