Proteomic analysis of nitrate-dependent acetone degradation by Alicycliphilus denitrificans strain BC
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/41757 |
Resumo: | Alicycliphilus denitrificans strain BC grows anaerobically on acetone with nitrate as electron acceptor. Comparative proteomics of cultures of A. denitrificans strain BC grown on either acetone or acetate with nitrate was performed to study the enzymes involved in the acetone degradation pathway. In the proposed acetone degradation pathway, an acetone carboxylase converts acetone to acetoacetate, an AMP-dependent synthetase/ligase converts acetoacetate to acetoacetyl-CoA, and an acetyl-CoA acetyltransferase cleaves acetoacetyl-CoA to two acetyl-CoA. We also found a putative aldehyde dehydrogenase associated with acetone degradation. This enzyme functioned as a -hydroxybutyrate dehydrogenase catalyzing the conversion of surplus acetoacetate to -hydroxybutyrate that may be converted to the energy and carbon storage compound, poly--hydroxybutyrate. Accordingly, we confirmed the formation of poly-?-hydroxybutyrate in acetone-grown cells of strain BC. Our findings provide insight in nitrate-dependent acetone degradation that is activated by carboxylation of acetone. This will aid studies of similar pathways found in other microorganisms degrading acetone with nitrate or sulfate as electron acceptor. |
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Proteomic analysis of nitrate-dependent acetone degradation by Alicycliphilus denitrificans strain BCAlicycliphilus denitrificansAcetoneProteomeAnaerobicNitrateβ-hydroxybutyratebeta-hydroxybutyrateScience & TechnologyAlicycliphilus denitrificans strain BC grows anaerobically on acetone with nitrate as electron acceptor. Comparative proteomics of cultures of A. denitrificans strain BC grown on either acetone or acetate with nitrate was performed to study the enzymes involved in the acetone degradation pathway. In the proposed acetone degradation pathway, an acetone carboxylase converts acetone to acetoacetate, an AMP-dependent synthetase/ligase converts acetoacetate to acetoacetyl-CoA, and an acetyl-CoA acetyltransferase cleaves acetoacetyl-CoA to two acetyl-CoA. We also found a putative aldehyde dehydrogenase associated with acetone degradation. This enzyme functioned as a -hydroxybutyrate dehydrogenase catalyzing the conversion of surplus acetoacetate to -hydroxybutyrate that may be converted to the energy and carbon storage compound, poly--hydroxybutyrate. Accordingly, we confirmed the formation of poly-?-hydroxybutyrate in acetone-grown cells of strain BC. Our findings provide insight in nitrate-dependent acetone degradation that is activated by carboxylation of acetone. This will aid studies of similar pathways found in other microorganisms degrading acetone with nitrate or sulfate as electron acceptor.This work was supported by the Technology Foundation, the Applied Science Division (STW) of the Netherlands Organization for Scientific Research (NWO) [project 08053]. Additional funding was provided by BE-BASIC [grant F08.004.01 to SA], an ERC grant [project 323009 to AJMS] and the Gravitation grant [project 024.002.002 to AJMS] of the Netherlands Ministry of Education, Culture and Science and NWO.Oxford University PressUniversidade do MinhoOosterkamp, Margreet J.Boeren, SjefAtashgahi, SiavashPlugge, Caroline M.Schaap, Peter J.Stams, Alfons Johannes Maria20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/41757engOosterkamp, Margreet J.; Boeren, Sjef; Atashgahi, Siavash; Plugge, Caroline M.; Schaap, Peter J.; Stams, A. J. M., Proteomic analysis of nitrate-dependent acetone degradation by Alicycliphilus denitrificans strain BC. FEMS Microbiology Letters, 362(11), 1-7, 20150378-109710.1093/femsle/fnv08025977262http://femsle.oxfordjournals.org/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:49:37Zoai:repositorium.sdum.uminho.pt:1822/41757Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:48:06.614404Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Proteomic analysis of nitrate-dependent acetone degradation by Alicycliphilus denitrificans strain BC |
title |
Proteomic analysis of nitrate-dependent acetone degradation by Alicycliphilus denitrificans strain BC |
spellingShingle |
Proteomic analysis of nitrate-dependent acetone degradation by Alicycliphilus denitrificans strain BC Oosterkamp, Margreet J. Alicycliphilus denitrificans Acetone Proteome Anaerobic Nitrate β-hydroxybutyrate beta-hydroxybutyrate Science & Technology |
title_short |
Proteomic analysis of nitrate-dependent acetone degradation by Alicycliphilus denitrificans strain BC |
title_full |
Proteomic analysis of nitrate-dependent acetone degradation by Alicycliphilus denitrificans strain BC |
title_fullStr |
Proteomic analysis of nitrate-dependent acetone degradation by Alicycliphilus denitrificans strain BC |
title_full_unstemmed |
Proteomic analysis of nitrate-dependent acetone degradation by Alicycliphilus denitrificans strain BC |
title_sort |
Proteomic analysis of nitrate-dependent acetone degradation by Alicycliphilus denitrificans strain BC |
author |
Oosterkamp, Margreet J. |
author_facet |
Oosterkamp, Margreet J. Boeren, Sjef Atashgahi, Siavash Plugge, Caroline M. Schaap, Peter J. Stams, Alfons Johannes Maria |
author_role |
author |
author2 |
Boeren, Sjef Atashgahi, Siavash Plugge, Caroline M. Schaap, Peter J. Stams, Alfons Johannes Maria |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Oosterkamp, Margreet J. Boeren, Sjef Atashgahi, Siavash Plugge, Caroline M. Schaap, Peter J. Stams, Alfons Johannes Maria |
dc.subject.por.fl_str_mv |
Alicycliphilus denitrificans Acetone Proteome Anaerobic Nitrate β-hydroxybutyrate beta-hydroxybutyrate Science & Technology |
topic |
Alicycliphilus denitrificans Acetone Proteome Anaerobic Nitrate β-hydroxybutyrate beta-hydroxybutyrate Science & Technology |
description |
Alicycliphilus denitrificans strain BC grows anaerobically on acetone with nitrate as electron acceptor. Comparative proteomics of cultures of A. denitrificans strain BC grown on either acetone or acetate with nitrate was performed to study the enzymes involved in the acetone degradation pathway. In the proposed acetone degradation pathway, an acetone carboxylase converts acetone to acetoacetate, an AMP-dependent synthetase/ligase converts acetoacetate to acetoacetyl-CoA, and an acetyl-CoA acetyltransferase cleaves acetoacetyl-CoA to two acetyl-CoA. We also found a putative aldehyde dehydrogenase associated with acetone degradation. This enzyme functioned as a -hydroxybutyrate dehydrogenase catalyzing the conversion of surplus acetoacetate to -hydroxybutyrate that may be converted to the energy and carbon storage compound, poly--hydroxybutyrate. Accordingly, we confirmed the formation of poly-?-hydroxybutyrate in acetone-grown cells of strain BC. Our findings provide insight in nitrate-dependent acetone degradation that is activated by carboxylation of acetone. This will aid studies of similar pathways found in other microorganisms degrading acetone with nitrate or sulfate as electron acceptor. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/41757 |
url |
http://hdl.handle.net/1822/41757 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oosterkamp, Margreet J.; Boeren, Sjef; Atashgahi, Siavash; Plugge, Caroline M.; Schaap, Peter J.; Stams, A. J. M., Proteomic analysis of nitrate-dependent acetone degradation by Alicycliphilus denitrificans strain BC. FEMS Microbiology Letters, 362(11), 1-7, 2015 0378-1097 10.1093/femsle/fnv080 25977262 http://femsle.oxfordjournals.org/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Oxford University Press |
publisher.none.fl_str_mv |
Oxford University Press |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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