Bamboo fibre processing: insights into hemicellulase and cellulase substrate accessibility
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/22629 |
Resumo: | A biological approach for degumming bamboo substrates has been assessed. The ability of various commercially available enzymes, including cellulase, xylanase, pectinase and mannanase, to hydrolyze bamboo powders was investigated. In addition, a commercial cellulase preparation was applied onto bamboo fibre bundles obtained by natural retting. It was found that almost all enzymes applied can use bamboo material as a substrate. Mild autoclave pre-treatment can enhance reducing sugar yield from different enzyme treatments. A most pronounced effect was observed with cellulase treatment in which the hydrolysis degree increased 1.7 fold as measured by reducing sugars for autoclave pre-treated bamboo powders versus non-treated powders after only a short period of incubation. The combined treatment of hemicellulase preparations showed no effect on the hydrolysis of bamboo substrates. The effect of autoclave pre-treatment on cellulase-treated samples was confirmed by the increase of sugar yield, protein absorption as well as by the enhancement of surface modification and enzyme penetration observed by CLSM (confocal laser scanning microscopy). This work establishes a base for future studies to develop enzymatic hydrolysis of bamboo materials, making them suitable for textile processing. |
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Bamboo fibre processing: insights into hemicellulase and cellulase substrate accessibilityBambooCellulaseXylanasePectinaseMannanaseAutoclaveCLSMScience & TechnologyA biological approach for degumming bamboo substrates has been assessed. The ability of various commercially available enzymes, including cellulase, xylanase, pectinase and mannanase, to hydrolyze bamboo powders was investigated. In addition, a commercial cellulase preparation was applied onto bamboo fibre bundles obtained by natural retting. It was found that almost all enzymes applied can use bamboo material as a substrate. Mild autoclave pre-treatment can enhance reducing sugar yield from different enzyme treatments. A most pronounced effect was observed with cellulase treatment in which the hydrolysis degree increased 1.7 fold as measured by reducing sugars for autoclave pre-treated bamboo powders versus non-treated powders after only a short period of incubation. The combined treatment of hemicellulase preparations showed no effect on the hydrolysis of bamboo substrates. The effect of autoclave pre-treatment on cellulase-treated samples was confirmed by the increase of sugar yield, protein absorption as well as by the enhancement of surface modification and enzyme penetration observed by CLSM (confocal laser scanning microscopy). This work establishes a base for future studies to develop enzymatic hydrolysis of bamboo materials, making them suitable for textile processing.This work was made possible by support from the earmarked fund for Modern Agro-industry Technology Research System (nycytx-19-E23), the European Union Biorenew Project [Sixth Framework Programme (FP6-2004-NMP-NI-4)] and the Fundamental Research Funds for the Central Universities (JUSRP211A02).Informa HealthcareUniversidade do MinhoFu, JiajiaNyanhongo, Gibson S.Silva, Carla Manuela Pereira Marinho daCardinale, MassimilianoPrasetyo, E. N.Yu, ChongwenPaulo, Artur CavacoGübitz, Georg M.20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/22629eng1024-242210.3109/10242422.2012.644440http://dx.doi.org/10.3109/10242422.2012.644440info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:00:17Zoai:repositorium.sdum.uminho.pt:1822/22629Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:50:09.923670Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bamboo fibre processing: insights into hemicellulase and cellulase substrate accessibility |
title |
Bamboo fibre processing: insights into hemicellulase and cellulase substrate accessibility |
spellingShingle |
Bamboo fibre processing: insights into hemicellulase and cellulase substrate accessibility Fu, Jiajia Bamboo Cellulase Xylanase Pectinase Mannanase Autoclave CLSM Science & Technology |
title_short |
Bamboo fibre processing: insights into hemicellulase and cellulase substrate accessibility |
title_full |
Bamboo fibre processing: insights into hemicellulase and cellulase substrate accessibility |
title_fullStr |
Bamboo fibre processing: insights into hemicellulase and cellulase substrate accessibility |
title_full_unstemmed |
Bamboo fibre processing: insights into hemicellulase and cellulase substrate accessibility |
title_sort |
Bamboo fibre processing: insights into hemicellulase and cellulase substrate accessibility |
author |
Fu, Jiajia |
author_facet |
Fu, Jiajia Nyanhongo, Gibson S. Silva, Carla Manuela Pereira Marinho da Cardinale, Massimiliano Prasetyo, E. N. Yu, Chongwen Paulo, Artur Cavaco Gübitz, Georg M. |
author_role |
author |
author2 |
Nyanhongo, Gibson S. Silva, Carla Manuela Pereira Marinho da Cardinale, Massimiliano Prasetyo, E. N. Yu, Chongwen Paulo, Artur Cavaco Gübitz, Georg M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Fu, Jiajia Nyanhongo, Gibson S. Silva, Carla Manuela Pereira Marinho da Cardinale, Massimiliano Prasetyo, E. N. Yu, Chongwen Paulo, Artur Cavaco Gübitz, Georg M. |
dc.subject.por.fl_str_mv |
Bamboo Cellulase Xylanase Pectinase Mannanase Autoclave CLSM Science & Technology |
topic |
Bamboo Cellulase Xylanase Pectinase Mannanase Autoclave CLSM Science & Technology |
description |
A biological approach for degumming bamboo substrates has been assessed. The ability of various commercially available enzymes, including cellulase, xylanase, pectinase and mannanase, to hydrolyze bamboo powders was investigated. In addition, a commercial cellulase preparation was applied onto bamboo fibre bundles obtained by natural retting. It was found that almost all enzymes applied can use bamboo material as a substrate. Mild autoclave pre-treatment can enhance reducing sugar yield from different enzyme treatments. A most pronounced effect was observed with cellulase treatment in which the hydrolysis degree increased 1.7 fold as measured by reducing sugars for autoclave pre-treated bamboo powders versus non-treated powders after only a short period of incubation. The combined treatment of hemicellulase preparations showed no effect on the hydrolysis of bamboo substrates. The effect of autoclave pre-treatment on cellulase-treated samples was confirmed by the increase of sugar yield, protein absorption as well as by the enhancement of surface modification and enzyme penetration observed by CLSM (confocal laser scanning microscopy). This work establishes a base for future studies to develop enzymatic hydrolysis of bamboo materials, making them suitable for textile processing. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2012-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/22629 |
url |
http://hdl.handle.net/1822/22629 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1024-2422 10.3109/10242422.2012.644440 http://dx.doi.org/10.3109/10242422.2012.644440 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Informa Healthcare |
publisher.none.fl_str_mv |
Informa Healthcare |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799132269397409792 |