Rat prostate: practical tips for ultrasonographic monitoring
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/31027 https://doi.org/10.1111/eci.13370 |
Resumo: | Background: Prostate is the largest accessory gland of the male reproductive tract. The prostate of men over 40 years- old is frequently affected by several pathologies, like benign prostate hyperplasia and cancer. Rats have been used as model to study prostate cancer. This study intended to address the usefulness of ultrasonography for rat prostate monitoring. Materials and Methods: Eight male Wistar Unilever rats were acquired from Charles River Laboratories and main- tained under controlled conditions of temperature, humidity, air system filtration and light/dark cycle. The prostate was evaluated by ultrasonography in awake animals. The animals were restrained by a researcher and placed in supine position. The skin of the inguinal region was shaved using a machine clipper (AESCULAP® GT420 Isis, USA). A real-time scan- ner (Logic P6®, GE, USA) and a 12 MHz linear transducer were used. Acoustic gel (Parker Laboratories Inc., USA) was applied. A complete transverse scan using B mode was per- formed from the cranial to the caudal region of the prostate, and a sagittal scan was performed moving the probe from the right to the left side. Procedures were approved by the Portuguese Ethics Committee (no.021326). Results: Prostate was easily evaluated by ultrasonography in all animals. In the transverse scan, the urinary bladder presents as a round to oval shape filled with urine (anechoic structure) and the prostate lobes were visible around it. The ventral prostate lobes appear as hypoechoic elongated struc- tures (one right and one left) with a hyperechoic capsule, placed ventrally to the urinary bladder. In this scan, the dorsal prostate was observed close to the urinary bladder neck, as a round hypoechoic structure with a hyperechoic capsule, dor- sally to the urinary bladder. In the sagittal scan, the urinary bladder was observed as an elongated structure filled with urine (anechoic content). The ventral prostate lobes were occasionally observed ventrally to the neck of the urinary bladder, as previously described. The dorsal prostate was ob- served dorsally to the neck of the urinary bladder, presenting as a round to elongated shape, with a hypoechoic appearance and a hyperechoic capsule.Conclusions: The ultrasonography is a non-invasive and ac- cessible tool for prostate monitoring in the rat model. |
id |
RCAP_c93b1dc7460b109269af5b2058a41d40 |
---|---|
oai_identifier_str |
oai:dspace.uevora.pt:10174/31027 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Rat prostate: practical tips for ultrasonographic monitoringBackground: Prostate is the largest accessory gland of the male reproductive tract. The prostate of men over 40 years- old is frequently affected by several pathologies, like benign prostate hyperplasia and cancer. Rats have been used as model to study prostate cancer. This study intended to address the usefulness of ultrasonography for rat prostate monitoring. Materials and Methods: Eight male Wistar Unilever rats were acquired from Charles River Laboratories and main- tained under controlled conditions of temperature, humidity, air system filtration and light/dark cycle. The prostate was evaluated by ultrasonography in awake animals. The animals were restrained by a researcher and placed in supine position. The skin of the inguinal region was shaved using a machine clipper (AESCULAP® GT420 Isis, USA). A real-time scan- ner (Logic P6®, GE, USA) and a 12 MHz linear transducer were used. Acoustic gel (Parker Laboratories Inc., USA) was applied. A complete transverse scan using B mode was per- formed from the cranial to the caudal region of the prostate, and a sagittal scan was performed moving the probe from the right to the left side. Procedures were approved by the Portuguese Ethics Committee (no.021326). Results: Prostate was easily evaluated by ultrasonography in all animals. In the transverse scan, the urinary bladder presents as a round to oval shape filled with urine (anechoic structure) and the prostate lobes were visible around it. The ventral prostate lobes appear as hypoechoic elongated struc- tures (one right and one left) with a hyperechoic capsule, placed ventrally to the urinary bladder. In this scan, the dorsal prostate was observed close to the urinary bladder neck, as a round hypoechoic structure with a hyperechoic capsule, dor- sally to the urinary bladder. In the sagittal scan, the urinary bladder was observed as an elongated structure filled with urine (anechoic content). The ventral prostate lobes were occasionally observed ventrally to the neck of the urinary bladder, as previously described. The dorsal prostate was ob- served dorsally to the neck of the urinary bladder, presenting as a round to elongated shape, with a hypoechoic appearance and a hyperechoic capsule.Conclusions: The ultrasonography is a non-invasive and ac- cessible tool for prostate monitoring in the rat model.European Journal of Clinical Investigation2022-01-31T16:18:31Z2022-01-312020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/31027http://hdl.handle.net/10174/31027https://doi.org/10.1111/eci.13370engFaustino-Rocha AI, Ferreira R, Pires MJ, Fardilha M, Oliveira PA, Ginja M. 2020. Rat prostate: practical tips for ultrasonographic monitoring. European Journal of Clinical Investigation 50 (1): 75.75https://onlinelibrary.wiley.com/toc/13652362/2020/50/S1501anafaustino@uevora.ptndndndndndFaustino-Rocha, Ana IsabelFerreira, RitaPires, Maria JoãoFardilha, MargaridaOliveira, Paula AlexandraGinja, Márioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:27:37Zoai:dspace.uevora.pt:10174/31027Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:19:34.234514Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Rat prostate: practical tips for ultrasonographic monitoring |
title |
Rat prostate: practical tips for ultrasonographic monitoring |
spellingShingle |
Rat prostate: practical tips for ultrasonographic monitoring Faustino-Rocha, Ana Isabel |
title_short |
Rat prostate: practical tips for ultrasonographic monitoring |
title_full |
Rat prostate: practical tips for ultrasonographic monitoring |
title_fullStr |
Rat prostate: practical tips for ultrasonographic monitoring |
title_full_unstemmed |
Rat prostate: practical tips for ultrasonographic monitoring |
title_sort |
Rat prostate: practical tips for ultrasonographic monitoring |
author |
Faustino-Rocha, Ana Isabel |
author_facet |
Faustino-Rocha, Ana Isabel Ferreira, Rita Pires, Maria João Fardilha, Margarida Oliveira, Paula Alexandra Ginja, Mário |
author_role |
author |
author2 |
Ferreira, Rita Pires, Maria João Fardilha, Margarida Oliveira, Paula Alexandra Ginja, Mário |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Faustino-Rocha, Ana Isabel Ferreira, Rita Pires, Maria João Fardilha, Margarida Oliveira, Paula Alexandra Ginja, Mário |
description |
Background: Prostate is the largest accessory gland of the male reproductive tract. The prostate of men over 40 years- old is frequently affected by several pathologies, like benign prostate hyperplasia and cancer. Rats have been used as model to study prostate cancer. This study intended to address the usefulness of ultrasonography for rat prostate monitoring. Materials and Methods: Eight male Wistar Unilever rats were acquired from Charles River Laboratories and main- tained under controlled conditions of temperature, humidity, air system filtration and light/dark cycle. The prostate was evaluated by ultrasonography in awake animals. The animals were restrained by a researcher and placed in supine position. The skin of the inguinal region was shaved using a machine clipper (AESCULAP® GT420 Isis, USA). A real-time scan- ner (Logic P6®, GE, USA) and a 12 MHz linear transducer were used. Acoustic gel (Parker Laboratories Inc., USA) was applied. A complete transverse scan using B mode was per- formed from the cranial to the caudal region of the prostate, and a sagittal scan was performed moving the probe from the right to the left side. Procedures were approved by the Portuguese Ethics Committee (no.021326). Results: Prostate was easily evaluated by ultrasonography in all animals. In the transverse scan, the urinary bladder presents as a round to oval shape filled with urine (anechoic structure) and the prostate lobes were visible around it. The ventral prostate lobes appear as hypoechoic elongated struc- tures (one right and one left) with a hyperechoic capsule, placed ventrally to the urinary bladder. In this scan, the dorsal prostate was observed close to the urinary bladder neck, as a round hypoechoic structure with a hyperechoic capsule, dor- sally to the urinary bladder. In the sagittal scan, the urinary bladder was observed as an elongated structure filled with urine (anechoic content). The ventral prostate lobes were occasionally observed ventrally to the neck of the urinary bladder, as previously described. The dorsal prostate was ob- served dorsally to the neck of the urinary bladder, presenting as a round to elongated shape, with a hypoechoic appearance and a hyperechoic capsule.Conclusions: The ultrasonography is a non-invasive and ac- cessible tool for prostate monitoring in the rat model. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01T00:00:00Z 2022-01-31T16:18:31Z 2022-01-31 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/31027 http://hdl.handle.net/10174/31027 https://doi.org/10.1111/eci.13370 |
url |
http://hdl.handle.net/10174/31027 https://doi.org/10.1111/eci.13370 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Faustino-Rocha AI, Ferreira R, Pires MJ, Fardilha M, Oliveira PA, Ginja M. 2020. Rat prostate: practical tips for ultrasonographic monitoring. European Journal of Clinical Investigation 50 (1): 75. 75 https://onlinelibrary.wiley.com/toc/13652362/2020/50/S1 50 1 anafaustino@uevora.pt nd nd nd nd nd |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
European Journal of Clinical Investigation |
publisher.none.fl_str_mv |
European Journal of Clinical Investigation |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799136677082431488 |