Influence of Extracellular Mimicked Hierarchical Nano-Micro-Topography on the Bacteria/Abiotic Interface
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/106629 https://doi.org/10.3390/polym12040828 |
Resumo: | The study of interfaces between engineered surfaces and prokaryotic cells is a subject whose actual relevance has been reinforced by the current outbreaks due to unknown viruses and antibiotic-resistant bacteria. Studies aiming at the development of antibacterial surfaces are based on two pillars: surface chemistry or topographical cues. This work reports the study of only the topographic aspect by the development of thin films of polyamide, which present attractive surface chemistry for bacterial adhesion. The same chemistry with only nano- or hierarchical nano- and micro-topography that mimics the extracellular matrix is obtained by sputter-depositing the thin films onto Si and polydimethylsiloxane (PDMS), respectively. The surface average roughness of the Si-modified surfaces was around 1 nm, while the hierarchical topography presented values from 750 to 1000 nm, with wavelengths and amplitudes ranging from 15-30 µm and 1-3 µm, respectively, depending on the deposition parameters. The surface topography, wettability, surface charge, and mechanical properties were determined and related to interface performance with two Gram+ and two Gram- bacterial strains. The overall results show that surfaces with only nano-topographic features present less density of bacteria, regardless of their cell wall composition or cell shape, if the appropriate surface chemistry is present. |
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Influence of Extracellular Mimicked Hierarchical Nano-Micro-Topography on the Bacteria/Abiotic Interfacenano-micro-wrinkleshierarchical topographiesmimic extracellular matricespolyamide thin filmsprokaryotic cellsThe study of interfaces between engineered surfaces and prokaryotic cells is a subject whose actual relevance has been reinforced by the current outbreaks due to unknown viruses and antibiotic-resistant bacteria. Studies aiming at the development of antibacterial surfaces are based on two pillars: surface chemistry or topographical cues. This work reports the study of only the topographic aspect by the development of thin films of polyamide, which present attractive surface chemistry for bacterial adhesion. The same chemistry with only nano- or hierarchical nano- and micro-topography that mimics the extracellular matrix is obtained by sputter-depositing the thin films onto Si and polydimethylsiloxane (PDMS), respectively. The surface average roughness of the Si-modified surfaces was around 1 nm, while the hierarchical topography presented values from 750 to 1000 nm, with wavelengths and amplitudes ranging from 15-30 µm and 1-3 µm, respectively, depending on the deposition parameters. The surface topography, wettability, surface charge, and mechanical properties were determined and related to interface performance with two Gram+ and two Gram- bacterial strains. The overall results show that surfaces with only nano-topographic features present less density of bacteria, regardless of their cell wall composition or cell shape, if the appropriate surface chemistry is present.MDPI2020-04-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/106629http://hdl.handle.net/10316/106629https://doi.org/10.3390/polym12040828eng2073-4360Ferreira, SílviaPiedade, A. P.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-04-13T09:45:42Zoai:estudogeral.uc.pt:10316/106629Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:23:03.138546Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Influence of Extracellular Mimicked Hierarchical Nano-Micro-Topography on the Bacteria/Abiotic Interface |
title |
Influence of Extracellular Mimicked Hierarchical Nano-Micro-Topography on the Bacteria/Abiotic Interface |
spellingShingle |
Influence of Extracellular Mimicked Hierarchical Nano-Micro-Topography on the Bacteria/Abiotic Interface Ferreira, Sílvia nano-micro-wrinkles hierarchical topographies mimic extracellular matrices polyamide thin films prokaryotic cells |
title_short |
Influence of Extracellular Mimicked Hierarchical Nano-Micro-Topography on the Bacteria/Abiotic Interface |
title_full |
Influence of Extracellular Mimicked Hierarchical Nano-Micro-Topography on the Bacteria/Abiotic Interface |
title_fullStr |
Influence of Extracellular Mimicked Hierarchical Nano-Micro-Topography on the Bacteria/Abiotic Interface |
title_full_unstemmed |
Influence of Extracellular Mimicked Hierarchical Nano-Micro-Topography on the Bacteria/Abiotic Interface |
title_sort |
Influence of Extracellular Mimicked Hierarchical Nano-Micro-Topography on the Bacteria/Abiotic Interface |
author |
Ferreira, Sílvia |
author_facet |
Ferreira, Sílvia Piedade, A. P. |
author_role |
author |
author2 |
Piedade, A. P. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Ferreira, Sílvia Piedade, A. P. |
dc.subject.por.fl_str_mv |
nano-micro-wrinkles hierarchical topographies mimic extracellular matrices polyamide thin films prokaryotic cells |
topic |
nano-micro-wrinkles hierarchical topographies mimic extracellular matrices polyamide thin films prokaryotic cells |
description |
The study of interfaces between engineered surfaces and prokaryotic cells is a subject whose actual relevance has been reinforced by the current outbreaks due to unknown viruses and antibiotic-resistant bacteria. Studies aiming at the development of antibacterial surfaces are based on two pillars: surface chemistry or topographical cues. This work reports the study of only the topographic aspect by the development of thin films of polyamide, which present attractive surface chemistry for bacterial adhesion. The same chemistry with only nano- or hierarchical nano- and micro-topography that mimics the extracellular matrix is obtained by sputter-depositing the thin films onto Si and polydimethylsiloxane (PDMS), respectively. The surface average roughness of the Si-modified surfaces was around 1 nm, while the hierarchical topography presented values from 750 to 1000 nm, with wavelengths and amplitudes ranging from 15-30 µm and 1-3 µm, respectively, depending on the deposition parameters. The surface topography, wettability, surface charge, and mechanical properties were determined and related to interface performance with two Gram+ and two Gram- bacterial strains. The overall results show that surfaces with only nano-topographic features present less density of bacteria, regardless of their cell wall composition or cell shape, if the appropriate surface chemistry is present. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/106629 http://hdl.handle.net/10316/106629 https://doi.org/10.3390/polym12040828 |
url |
http://hdl.handle.net/10316/106629 https://doi.org/10.3390/polym12040828 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2073-4360 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134118705889280 |