Zebrafish : a new model of Parkinson's disease

Detalhes bibliográficos
Autor(a) principal: Fonseca, Tomás Ribeiro da Silva Lopes da, 1987-
Data de Publicação: 2010
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/2214
Resumo: Tese de mestrado, Neurociências, Faculdade de Medicina, Universidade de Lisboa, 2010
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spelling Zebrafish : a new model of Parkinson's diseaseDoença de ParkinsonPeixe-zebraTrifosfato de adenosinaModelos genéticosBioinformáticaTeses de mestrado - 2010Alpha-synucleinTese de mestrado, Neurociências, Faculdade de Medicina, Universidade de Lisboa, 2010Parkinson´s disease (PD) is the second most common neurodegenerative disease after Alzheimer’s disease, affecting around 2% of the population with more than 65 years. The first gene implicated with PD was SNCA that encodes to alpha-synuclein. At the neuronal level, this protein was found in the Lewy Bodies, one of the pathologic hallmarks of the disease and it has been identified both in sporadic and familiar cases of PD. Its role in the disease is still unclear. Besides alpha-synuclein, other 15 genetic loci have been associated to PD. To clarify the role of alpha-synuclein in PD, several in vivo models have been developed, expressing wild-type and PD-associated mutant forms of the protein. However, none of the existing models fully recapitulates all of the hallmarks of the disease. To circumvent this limitation, new models must be developed. Zebrafish has been widely used as an animal model for studying several human disorders because, besides being a vertebrate, it has all the molecular background and bioimaging characteristics suitable for these studies. Here, we started to generate a Zebrafish transgenic model for human alpha-synuclein. In addition, through bioinformatic analysis, we found that four PD related genes encoding ATP13a2, Lrrk2, Synphilin-1, and Glucocerebrosidase, display high homology rates when compared to the human gene sequences. Moreover, despite a high homology in the entire sequence, specific conserved domains sequences are also highly homologous, suggesting a conserved functionality of the genes. In situ hybridization of ATP13a2 revealed that, although initially expressed in whole embryo’s body, it becomes more restricted to the brain area along the embryonic development. The generation of this novel PD transgenic model will allow further studies to better understand the mechanisms underlying the disease pathology. Taken together, these results will provide a novel and powerful system to explore more the molecular basis of PD and to open novel avenues for therapeutic intervention.Outeiro, Tiago Fleming, 1976-Correia, AnaRepositório da Universidade de LisboaFonseca, Tomás Ribeiro da Silva Lopes da, 1987-2010-12-09T14:58:00Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10451/2214enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:42:29Zoai:repositorio.ul.pt:10451/2214Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:28:39.859982Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Zebrafish : a new model of Parkinson's disease
title Zebrafish : a new model of Parkinson's disease
spellingShingle Zebrafish : a new model of Parkinson's disease
Fonseca, Tomás Ribeiro da Silva Lopes da, 1987-
Doença de Parkinson
Peixe-zebra
Trifosfato de adenosina
Modelos genéticos
Bioinformática
Teses de mestrado - 2010
Alpha-synuclein
title_short Zebrafish : a new model of Parkinson's disease
title_full Zebrafish : a new model of Parkinson's disease
title_fullStr Zebrafish : a new model of Parkinson's disease
title_full_unstemmed Zebrafish : a new model of Parkinson's disease
title_sort Zebrafish : a new model of Parkinson's disease
author Fonseca, Tomás Ribeiro da Silva Lopes da, 1987-
author_facet Fonseca, Tomás Ribeiro da Silva Lopes da, 1987-
author_role author
dc.contributor.none.fl_str_mv Outeiro, Tiago Fleming, 1976-
Correia, Ana
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Fonseca, Tomás Ribeiro da Silva Lopes da, 1987-
dc.subject.por.fl_str_mv Doença de Parkinson
Peixe-zebra
Trifosfato de adenosina
Modelos genéticos
Bioinformática
Teses de mestrado - 2010
Alpha-synuclein
topic Doença de Parkinson
Peixe-zebra
Trifosfato de adenosina
Modelos genéticos
Bioinformática
Teses de mestrado - 2010
Alpha-synuclein
description Tese de mestrado, Neurociências, Faculdade de Medicina, Universidade de Lisboa, 2010
publishDate 2010
dc.date.none.fl_str_mv 2010-12-09T14:58:00Z
2010
2010-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/2214
url http://hdl.handle.net/10451/2214
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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